ID A0A2U2ZJV2_9ACTN Unreviewed; 491 AA.
AC A0A2U2ZJV2;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=DIZ27_02010 {ECO:0000313|EMBL:PWI12555.1};
OS Streptomyces sp. NWU339.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2185284 {ECO:0000313|EMBL:PWI12555.1, ECO:0000313|Proteomes:UP000245372};
RN [1] {ECO:0000313|EMBL:PWI12555.1, ECO:0000313|Proteomes:UP000245372}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NWU339 {ECO:0000313|EMBL:PWI12555.1,
RC ECO:0000313|Proteomes:UP000245372};
RA Adegboye M.F., Lobb B., Babalola O., Doxey A.C., Ma K.;
RT "Draft genomes of two novel cellulolytic Streptomyces strains isolated from
RT South African rhizosphere soils.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWI12555.1}.
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DR EMBL; QFRK01000001; PWI12555.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U2ZJV2; -.
DR REBASE; 268503; M1.Ssp339ORF2010P.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000245372; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:PWI12555.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000245372};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..129
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 140..451
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 491 AA; 54509 MW; 348442FCDE586C40 CRC64;
MESHLLGAAH LLRGLVDQAD FKSYIFPLLF YKRLCDVYSE EFDDALAHSD GDRDFAAKAD
QHRFVIPEGH RWEDVVAAPD EKLGRALTDA LRAIEKANPE TLDGIFGDTP WTNRDRIPPH
VLRALLNHFS ARFLGDAHTD SDLLGQAYEY LIKKFADLSN KKAGEFYTPR AVVELLVNIL
APKSGETVYD PACGTGGMLI EVTHHFARKG VAPQLLWGKI FGQEKNLTTS GIARMNLILH
GIEDFRVARG DTLREPRFFA ADGSLRAFDC VIANPPFSLA AWGEKGWARD PYGRGETGVP
PRSTADWAWV QHMLASMRPG TGRAVVVLPQ GILFRDGAEA RIRRKIIESD AIEAVIGLGE
RLFYGAPLAA CVLVLRANKP KDRQKKILFI DAADQLRKES KQNVLEAEHT SRILGWYRAF
ADIKERSRLV GLPEIEKNDG SLNIALYVGK DRSAGLPPLS EAVAALESAL VDAATAETEL
RDRLKEWRLL G
//
