ID A0A2U2ZLE6_9ACTN Unreviewed; 715 AA.
AC A0A2U2ZLE6;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=DI272_02460 {ECO:0000313|EMBL:PWI13121.1};
OS Streptomyces sp. Act143.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2200760 {ECO:0000313|EMBL:PWI13121.1, ECO:0000313|Proteomes:UP000245455};
RN [1] {ECO:0000313|EMBL:PWI13121.1, ECO:0000313|Proteomes:UP000245455}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Act143 {ECO:0000313|EMBL:PWI13121.1,
RC ECO:0000313|Proteomes:UP000245455};
RA Valanrebinro G., Kumari S., Rameshkumar N.;
RT "Actinomycin D producing novel Streptomyces isolated from the rhizosphere
RT of saline tolerant kaipad rice and its draft genome analysis.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|PROSITE-ProRule:PRU10059, ECO:0000256|RuleBase:RU361166}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWI13121.1}.
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DR EMBL; QFRX01000001; PWI13121.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U2ZLE6; -.
DR OrthoDB; 9808897at2; -.
DR Proteomes; UP000245455; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030247; F:polysaccharide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR CDD; cd02850; E_set_Cellulase_N; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.60.40.290; -; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001919; CBD2.
DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR InterPro; IPR012291; CBM2_carb-bd_dom_sf.
DR InterPro; IPR018366; CBM2_CS.
DR InterPro; IPR004197; Cellulase_Ig-like.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00553; CBM_2; 1.
DR Pfam; PF02927; CelD_N; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM00637; CBD_II; 1.
DR SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS51173; CBM2; 1.
DR PROSITE; PS00561; CBM2_A; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000245455};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..35
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 36..715
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5015372825"
FT DOMAIN 607..715
FT /note="CBM2"
FT /evidence="ECO:0000259|PROSITE:PS51173"
FT REGION 530..553
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 525
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 573
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 582
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 715 AA; 74567 MW; 121153A3493EEED3 CRC64;
MPAFTLLRRP SRPLTAALLT SSLLLGGFLA VPAAAAGTTA VRVNQVGYLP DGPKRATVVT
TAAQSLTWQL SNASGTVVAS GSTVPRGADT ASGQSVQVAD FSSYQGTGSG YVLRVDGSSS
DPFDIRANLY DGLRSDAMAF FYHQRSGIAI DASLVGSAYA RPAGHLGVSP NKGDTSVPCQ
SGVCDYTQDV RGGWYDAGDQ GKYVVNGGIS TWLVVNSFER AERVGKEAAL GDSTLSVPER
GNGVPDVLDE ARWELDFLMR MQVPAGKPYA GMAFHKIHDA AWTGIPTRPE QDAQPRELHR
PSTAATLNLA ATAAQCARVF RPYDAAYADR CLAAARRAWT AAQANPAMYA PDSDSTGGGA
YGDSQVSDEF YWAAAELYAT TGESAYRDAV TSSSWHTAAN AFTSYGFGWA DTAALGRLTL
ATVPNGLPAS DLARVRSSVV SAADGHLSTM AGQGYAVPIP SDGYFWGSNS EAADNAVVMA
VAGELTGDAR YRAGVLEALD YLLGRNALGQ SYVTGYGEKS SRNQHHRFWA HQSDSSLPNP
PAGSLAGGPN SGLQDPVAQE KLAGCAPAAC YIDDIGSYST NEVAVNWNAP LAWLAAYAAE
RTTDDGGQQP TGSCAVTYKV DNAWSNGFTA TVTVKNTGST AVDGWQLTWS YPGGQRVTSA
WNASVTQSGA DVTARNVDWN RTVAPGATVS FGVQGSLSGS NPSPTAFALN GSACD
//
