ID A0A2U2ZN77_9ACTN Unreviewed; 747 AA.
AC A0A2U2ZN77;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=DI272_05950 {ECO:0000313|EMBL:PWI13737.1};
OS Streptomyces sp. Act143.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2200760 {ECO:0000313|EMBL:PWI13737.1, ECO:0000313|Proteomes:UP000245455};
RN [1] {ECO:0000313|EMBL:PWI13737.1, ECO:0000313|Proteomes:UP000245455}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Act143 {ECO:0000313|EMBL:PWI13737.1,
RC ECO:0000313|Proteomes:UP000245455};
RA Valanrebinro G., Kumari S., Rameshkumar N.;
RT "Actinomycin D producing novel Streptomyces isolated from the rhizosphere
RT of saline tolerant kaipad rice and its draft genome analysis.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|PROSITE-ProRule:PRU10060, ECO:0000256|RuleBase:RU361166}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWI13737.1}.
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DR EMBL; QFRX01000001; PWI13737.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U2ZN77; -.
DR OrthoDB; 9808897at2; -.
DR Proteomes; UP000245455; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR CDD; cd02850; E_set_Cellulase_N; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR004197; Cellulase_Ig-like.
DR InterPro; IPR003305; CenC_carb-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF02018; CBM_4_9; 1.
DR Pfam; PF02927; CelD_N; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10060};
KW Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10060};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10060};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10060};
KW Reference proteome {ECO:0000313|Proteomes:UP000245455};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..29
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 30..747
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5015369998"
FT DOMAIN 33..154
FT /note="CBM-cenC"
FT /evidence="ECO:0000259|Pfam:PF02018"
FT DOMAIN 182..265
FT /note="Cellulase Ig-like"
FT /evidence="ECO:0000259|Pfam:PF02927"
FT DOMAIN 275..737
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 718
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 727
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 747 AA; 80277 MW; 0665F481DCBD85AA CRC64;
MKRRSTTLLS LTALLGAVLV AAPTSPATAD EVEQLKNGTF DTTTEPWWAT SNVTAGLSGG
RLCADVPGGT TNRWDAAVGQ NDVTLVKGET YKFAFTADGS PDGHVVRAIV GLQVAPYDTY
FEVSPQLSVS GNSYSYTFTS PVDATQAQVG FQLGGSADAW KFCMDDVSLL GGVPPEVYEP
DTGPRVRVNQ VAYLPSGPKN ATLVTDATGK LPWQLKNASG TVVAQGTTVP RGVDASSAQN
VHSIDFGAYK KAGTGYTLVA DGETSRPFDI GTGAYRQLRL DALKYYYTQR SGIAIRDDLR
PGYGRAAGHV DVAPNQGDSA VPCQPGVCDY KLDVTGGWYD AGDHGKYVVN GGISTWELLS
TYERALHART GQPAKLGDNT LAIPESGNKV PDILDETRWE LEFLLKMQVP AGQPLAGMAH
HKIHDEAWTG LPLLPSDDPQ KRELHPATTE ATLNLAATAA QAARLYKPYD KAFAAKTLAA
ARTAWTAALA HPDLHADPDD GTGGGAYPDT DATDEFYWAA AELYLTTGEK QFADHVLDSP
VHTADIFGPL GYDWARTAAA ARLDLATVPS KLPGRDKVRR SVVKGADRYL ATLKSQPYGM
PYAPTDNLYD WGSNHQILHN GIVIATAYDI TGATKYRDGA LQSVDYLFGR NALNMSYVTG
YGEVNSHNQH SRWYAHQLDP NLPNPPRGTL AGGPNSSIQD PYAQSKLQGC VGQFCYIDDI
QSWSTNEHTI NWNSALSRMA SFVADQT
//
