ID A0A2U3AKE8_9BACL Unreviewed; 1112 AA.
AC A0A2U3AKE8;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=DEX24_10575 {ECO:0000313|EMBL:PWI25010.1};
OS Kurthia sibirica.
OC Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae; Kurthia.
OX NCBI_TaxID=202750 {ECO:0000313|EMBL:PWI25010.1, ECO:0000313|Proteomes:UP000245938};
RN [1] {ECO:0000313|EMBL:PWI25010.1, ECO:0000313|Proteomes:UP000245938}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49154 {ECO:0000313|EMBL:PWI25010.1,
RC ECO:0000313|Proteomes:UP000245938};
RA Maclea K.S., Goen A.E.;
RT "Kurthia sibirica genome sequence.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWI25010.1}.
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DR EMBL; QFVR01000013; PWI25010.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U3AKE8; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000245938; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000245938}.
FT DOMAIN 494..765
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT BINDING 515..522
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1112 AA; 126639 MW; 3CDFEB3D5C557701 CRC64;
MPIDLNETTN VGTNDDLMEN LFGTRMDKPR VTPALFDLKL TGNGYGKVGY LNHLFANVKD
NSSLTPHLHG DKTGFKFGFK KFVPKKGDFI QYPGDILKAE LKKPNTYIER LNSTRTEFNG
NGCVTEEMSV IAPSLYTSHF DEFFVKHNNS NNPVEVSATS DLFNLKELKN IAENAVELED
IMKQNGKLTN KEEMTLKDTW NTLFVPNVQR THQVFTKTEV TYISKNRTVI VKDELDKKNP
TAPPKPKKTE KFDYFFAPTT NIAFYPNELA MNQLTPDKAY VIHFNQLTAN LNAVINLMKN
QKDKVFIIRI PTKGFEQNSK AKALILDNLT KLVELSILND PTDSPNIMLR FVELMENENL
RDALEHQAIN VRDHSTDMLI QLMDLVARNR VAYRIDTDKS AQLVHIALLT FSRIEALEES
WVTVEDYNRI YKKLKELQAI FDWSDVKLRQ LSSTQLRLML SENLYLLDVE RKNGQLYQFK
AGNQQVHDDF MQDPKYSTQQ KAIITTTEPL ATVSAGAGSG KSYTLIGRLE YLKAQGEDLS
RTMVLSFTNA AAQNITSRYP NIKSLTLGDL FNKIYSLTFP NQVLSHPTTF KNALSCVNVE
ANVFKRFNLK RMMEAKQLFI GLMDQLSPSG FVKPDVAAVL SVLSNFIAEY YEEAMALMNS
IHQTTLELQP IVIYNMLLNS PHLLQIPVEY DEIDFIITDE SQDISTFEYI LLLSLVTIRK
MNMMIIGDGS QTLYEFRNSN PRFLTAIEAS GVFESFRLTT NYRSKQAILN YANQFLQVID
ANKIAKIQLQ SNSLAPVTQQ DIDKAIKIKY VMHGDHKNER KQNETLGRFI QENADFSDWF
LSRVRNGEQV AFMSHTRKEL DILEESIGEL LSLHGLHNTQ MERIVTTREK PKCQLSDIIN
KKYEELIDFD VTQNYENELK DYFCDQILES FKNKAKAFVP SYVERVLNKI LSLGGVAATK
HEILVGNSDK RMLNGFVIQA LIDEEIRLNA MANFLNKQTN ATKDYSSVPI ILTTIHSAKG
LEFPHTVVIY NSMRKTAKKQ ETLRLYGVAL TRAKESEFII ELNDAIGLSK VATSDLVGMY
GTPIKTAKLR IDREAQGLSA GVTTYDDGEE EE
//
