ID A0A2U3PF67_9MYCO Unreviewed; 430 AA.
AC A0A2U3PF67;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE SubName: Full=Coenzyme F420-reducing hydrogenase, alpha subunit {ECO:0000313|EMBL:SPM42389.1};
DE Flags: Fragment;
GN ORFNames=MNAB215_4609 {ECO:0000313|EMBL:SPM42389.1};
OS Mycobacterium numidiamassiliense.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=1841861 {ECO:0000313|EMBL:SPM42389.1, ECO:0000313|Proteomes:UP000240424};
RN [1] {ECO:0000313|EMBL:SPM42389.1, ECO:0000313|Proteomes:UP000240424}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AB215 {ECO:0000313|EMBL:SPM42389.1,
RC ECO:0000313|Proteomes:UP000240424};
RG Urmite Genomes;
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
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DR EMBL; FUEZ01000004; SPM42389.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U3PF67; -.
DR STRING; 1841861.GCA_900157365_02929; -.
DR Proteomes; UP000240424; Unassembled WGS sequence.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR43600; COENZYME F420 HYDROGENASE, SUBUNIT ALPHA; 1.
DR PANTHER; PTHR43600:SF2; CYTOSOLIC NIFE-HYDROGENASE, ALPHA SUBUNIT; 1.
DR Pfam; PF00374; NiFeSe_Hases; 2.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
DR PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|PIRSR:PIRSR601501-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000240424}.
FT BINDING 46
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 65
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 68
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 68
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 371
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 415
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 418
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 421
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:SPM42389.1"
SQ SEQUENCE 430 AA; 46855 MW; 80341A5D1A0F1FFD CRC64;
VTPETRTLSV GTLARVEGEG ALHVTLKDGE LEGVELNIYE PPRFFEAFLR GRAYTEPPDL
TARICGICPV AYQVSACNAI EEACGVQVDD DLVALRRLLY CGEWINSHAL HIYLLHAPDF
LGYPDIVGMS RDHAEVVERG LSLKKAGNRL MEFVGGRAIH PVNVRLGGFY SVPARLEFRP
IAEQLRRALD SAVATVEWVA GFDFPDVELD HEFLALTASG RYPIENGTIA RSGGPSFAVA
DFTNHVAESQ VPYSTALHAT LDGGRHLTGP LARYSLNSPA LSPIAAQAAA DAGLLSECRN
PFRSIIVRAV EVVYAIEEAL RIIEEYQRPS RPFVDVPARA GVGHGVSEAP RGLLYHRYRI
DEDGLVSAAT IIPPTSQNQA AIEADLARIV SDNLSLDDAA LTTLCERLIR SYDPCISCST
HFLTLTVQRT
//
