ID A0A2U4CJW2_TURTR Unreviewed; 354 AA.
AC A0A2U4CJW2;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Protein-tyrosine sulfotransferase {ECO:0000256|RuleBase:RU365018};
DE EC=2.8.2.20 {ECO:0000256|RuleBase:RU365018};
GN Name=TPST2 {ECO:0000313|RefSeq:XP_019805736.1};
OS Tursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Whippomorpha; Cetacea; Odontoceti;
OC Delphinidae; Tursiops.
OX NCBI_TaxID=9739 {ECO:0000313|Proteomes:UP000245320, ECO:0000313|RefSeq:XP_019805736.1};
RN [1] {ECO:0000313|RefSeq:XP_019805736.1}
RP IDENTIFICATION.
RC TISSUE=Spleen {ECO:0000313|RefSeq:XP_019805736.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic
CC motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as
CC cosubstrate. {ECO:0000256|ARBA:ARBA00003886,
CC ECO:0000256|RuleBase:RU365018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3'-phosphoadenylyl sulfate + L-tyrosyl-[protein] = adenosine
CC 3',5'-bisphosphate + H(+) + O-sulfo-L-tyrosine-[protein];
CC Xref=Rhea:RHEA:16801, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:11688,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:46858, ChEBI:CHEBI:58339,
CC ChEBI:CHEBI:58343, ChEBI:CHEBI:65286; EC=2.8.2.20;
CC Evidence={ECO:0000256|ARBA:ARBA00000411,
CC ECO:0000256|RuleBase:RU365018};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}.
CC -!- SIMILARITY: Belongs to the protein sulfotransferase family.
CC {ECO:0000256|ARBA:ARBA00009988, ECO:0000256|RuleBase:RU365018}.
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DR RefSeq; XP_019805736.1; XM_019950177.2.
DR AlphaFoldDB; A0A2U4CJW2; -.
DR GeneID; 101326946; -.
DR OrthoDB; 5391764at2759; -.
DR Proteomes; UP000245320; Chromosome 13.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008476; F:protein-tyrosine sulfotransferase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR026634; TPST-like.
DR PANTHER; PTHR12788; PROTEIN-TYROSINE SULFOTRANSFERASE 2; 1.
DR PANTHER; PTHR12788:SF6; PROTEIN-TYROSINE SULFOTRANSFERASE 2; 1.
DR Pfam; PF13469; Sulfotransfer_3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000245320};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365018};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..354
FT /note="Protein-tyrosine sulfotransferase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5015502446"
SQ SEQUENCE 354 AA; 39447 MW; 61BF7B1760A16A7C CRC64;
MRLSVRRALL AAGFALALVL AVQLGQQVLE CRVALGGPRG PRRAMRPEQE DLVMVGTDHV
EYRYGKAMPL IFVGGVPRSG TTLMRAMLDA HPEVRCGEET RIIPRVLAMR QAWSKSGREK
LRLDEAGVTD EVLDAAMQAF ILEVIAKHGE PARVLCNKDP FTLKSSVYLS RLFPNSKFLL
MVRDGRASVH SMITRKVTIA GFDLSSYRDC LAKWNKAIEV MYAQCMEVGE DKCLPVYYEQ
LVLHPRRSLK LILDFLGISW SDSVLHHEDL IGKPGGVSLS NPSSLPRIER STDQVIKPVN
LEALSKWTGH IPGDVLRDMA QIAPMLARLG YDPYANPPNY GNPDPIVINN THRD
//
