ID A0A2U8FT32_9BURK Unreviewed; 608 AA.
AC A0A2U8FT32;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Bifunctional protein glk {ECO:0000256|ARBA:ARBA00022251};
DE EC=2.7.1.2 {ECO:0000256|ARBA:ARBA00012323};
GN ORFNames=DEH84_11885 {ECO:0000313|EMBL:AWI54047.1};
OS Aquabacterium olei.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Aquabacterium.
OX NCBI_TaxID=1296669 {ECO:0000313|EMBL:AWI54047.1, ECO:0000313|Proteomes:UP000244892};
RN [1] {ECO:0000313|EMBL:AWI54047.1, ECO:0000313|Proteomes:UP000244892}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 110486 {ECO:0000313|EMBL:AWI54047.1,
RC ECO:0000313|Proteomes:UP000244892};
RA Tang B., Chang J., Zhang L., Yang H.;
RT "complete genome sequence of Aquabacterium olei NBRC 110486.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001632};
CC -!- SIMILARITY: In the N-terminal section; belongs to the bacterial
CC glucokinase family. {ECO:0000256|ARBA:ARBA00007693}.
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DR EMBL; CP029210; AWI54047.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U8FT32; -.
DR KEGG; aon:DEH84_11885; -.
DR OrthoDB; 257751at2; -.
DR Proteomes; UP000244892; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0004340; F:glucokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-KW.
DR CDD; cd05013; SIS_RpiR; 1.
DR Gene3D; 3.30.420.40; -; 1.
DR Gene3D; 3.40.367.20; -; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR003836; Glucokinase.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR000281; HTH_RpiR.
DR InterPro; IPR035472; RpiR-like_SIS.
DR InterPro; IPR001347; SIS_dom.
DR InterPro; IPR046348; SIS_dom_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR47690; GLUCOKINASE; 1.
DR PANTHER; PTHR47690:SF1; GLUCOKINASE; 1.
DR Pfam; PF02685; Glucokinase; 1.
DR Pfam; PF01418; HTH_6; 1.
DR Pfam; PF01380; SIS; 1.
DR SUPFAM; SSF53067; Actin-like ATPase domain; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR SUPFAM; SSF53697; SIS domain; 1.
DR PROSITE; PS00356; HTH_LACI_1; 1.
DR PROSITE; PS51071; HTH_RPIR; 1.
DR PROSITE; PS51464; SIS; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:AWI54047.1};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Reference proteome {ECO:0000313|Proteomes:UP000244892};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AWI54047.1}.
FT DOMAIN 329..405
FT /note="HTH rpiR-type"
FT /evidence="ECO:0000259|PROSITE:PS51071"
FT DOMAIN 449..589
FT /note="SIS"
FT /evidence="ECO:0000259|PROSITE:PS51464"
SQ SEQUENCE 608 AA; 63608 MW; 5F5E799F822CF778 CRC64;
MDGSRLLALL DPHGRLTLCR PDGALQQGAP VVEQRACTGP AQLASALKAG WVAQAGRQAG
PLRHLALAVA GTVHDDEVVL THKGWRLSVS ALRESLSLET LLVVNDFTAL AMALPALPPE
ACVALGGGQA QPGGVIGLIG PGEGLGVSGL IPAEDRRITL GSEGGHISFA PADDTELAIL
QAAWRQWPHV SAERLVSAQG LRFIWQTLGP QADTPPDAFT VIDLAHAGEP RSAAAVAVFA
RMLGTVAADV ALMLGAVGGV YLGGELLPRM GPLLAAGGFR ARFESKGRFS AWLAAVPTVL
VTAPWPVFSG LEALLTHHLR DRGAALQDGG VMARIKSLRN ELSRAEQRVA DHLLAHPRAF
MNEPVAEIAR QAEVSQPTVI RFCRSLGFEG LSDFKLKLAS GLTGTIPLRH SQVMVDDAAP
DLSAKVLDNT VSAIVSLRDG LNGQAIGRAI ALLQQARRVE FHGVGNSHVV AVDGQYKFLR
FGIPATAHAD SALQAMAADL LGPGDVVVAV SRSGAMPDLI HAVDKALNAG AAVVAITASD
SPLARRATVT LAVDHVEGRT EQLSMVSRIL HLLMIDVLAV GVAMQRPGSV KSPFGLREDA
AAWISHAR
//
