ID A0A2U8GPV7_9RHOO Unreviewed; 647 AA.
AC A0A2U8GPV7;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Nitrous-oxide reductase {ECO:0000256|ARBA:ARBA00016560};
DE EC=1.7.2.4 {ECO:0000256|ARBA:ARBA00011896};
DE AltName: Full=N(2)OR {ECO:0000256|ARBA:ARBA00031077};
DE AltName: Full=N2O reductase {ECO:0000256|ARBA:ARBA00032847};
GN ORFNames=CEW83_11255 {ECO:0000313|EMBL:AWI75719.1};
OS Parazoarcus communis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Parazoarcus.
OX NCBI_TaxID=41977 {ECO:0000313|EMBL:AWI75719.1, ECO:0000313|Proteomes:UP000244930};
RN [1] {ECO:0000313|EMBL:AWI75719.1, ECO:0000313|Proteomes:UP000244930}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TSPY31 {ECO:0000313|EMBL:AWI75719.1,
RC ECO:0000313|Proteomes:UP000244930};
RA Woo J.-H., Kim H.-S.;
RT "Azoarcus.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Nitrous-oxide reductase is part of a bacterial respiratory
CC system which is activated under anaerobic conditions in the presence of
CC nitrate or nitrous oxide. {ECO:0000256|ARBA:ARBA00003034}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 Fe(III)-[cytochrome c] + H2O + N2 = 2 Fe(II)-[cytochrome c]
CC + 2 H(+) + nitrous oxide; Xref=Rhea:RHEA:43108, Rhea:RHEA-COMP:10350,
CC Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17045, ChEBI:CHEBI:17997, ChEBI:CHEBI:29033,
CC ChEBI:CHEBI:29034; EC=1.7.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00029370};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- COFACTOR:
CC Name=Cu cation; Xref=ChEBI:CHEBI:23378;
CC Evidence={ECO:0000256|ARBA:ARBA00001935};
CC -!- PATHWAY: Nitrogen metabolism; nitrate reduction (denitrification);
CC dinitrogen from nitrate: step 4/4. {ECO:0000256|ARBA:ARBA00004779}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000256|ARBA:ARBA00004418}.
CC -!- SIMILARITY: Belongs to the NosZ family.
CC {ECO:0000256|ARBA:ARBA00010372}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the cytochrome c
CC oxidase subunit 2 family. {ECO:0000256|ARBA:ARBA00006790}.
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DR EMBL; CP022187; AWI75719.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U8GPV7; -.
DR KEGG; acom:CEW83_11255; -.
DR UniPathway; UPA00652; UER00709.
DR Proteomes; UP000244930; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR GO; GO:0050304; F:nitrous-oxide reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0019333; P:denitrification pathway; IEA:UniProtKB-UniPathway.
DR CDD; cd04223; N2OR_C; 1.
DR Gene3D; 2.60.40.420; Cupredoxins - blue copper proteins; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR002429; CcO_II-like_C.
DR InterPro; IPR008972; Cupredoxin.
DR InterPro; IPR028096; EfeO_Cupredoxin.
DR InterPro; IPR011045; N2O_reductase_N.
DR InterPro; IPR034205; N2OR_C.
DR InterPro; IPR023644; NO_Rdtase.
DR InterPro; IPR041114; Nos_propeller.
DR InterPro; IPR041142; NOS_propeller_2.
DR InterPro; IPR006311; TAT_signal.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR NCBIfam; TIGR04244; nitrous_NosZ_RR; 1.
DR PANTHER; PTHR42838; CYTOCHROME C OXIDASE SUBUNIT II; 1.
DR PANTHER; PTHR42838:SF1; NITROUS-OXIDE REDUCTASE; 1.
DR Pfam; PF13473; Cupredoxin_1; 1.
DR Pfam; PF18764; nos_propeller; 1.
DR Pfam; PF18793; nos_propeller_2; 1.
DR SUPFAM; SSF49503; Cupredoxins; 1.
DR SUPFAM; SSF50974; Nitrous oxide reductase, N-terminal domain; 1.
DR PROSITE; PS50857; COX2_CUA; 1.
DR PROSITE; PS51318; TAT; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Periplasm {ECO:0000256|ARBA:ARBA00022764};
KW Reference proteome {ECO:0000313|Proteomes:UP000244930};
KW Signal {ECO:0000256|ARBA:ARBA00022729}.
FT DOMAIN 551..647
FT /note="Cytochrome oxidase subunit II copper A binding"
FT /evidence="ECO:0000259|PROSITE:PS50857"
SQ SEQUENCE 647 AA; 71042 MW; 453312CCBC56973E CRC64;
MNEAKMKPVG EMPDPGRRKF LNTAAFAGLA GAGLSVGLSA CNKETAPAPA ASTAPMAAAE
HLEVGKYEVA PGQLDEYYSF SSGGHSGECR IYGLPSGREL KRIPVFNMDC LVGWGTTNES
KAILGTKPNG TPKYTTGDTH HVHPSYTDGT YDGKYMFVND KIHGRLARIR MDTMETDKIT
EIPNIQGFHG TFPDKRDPVD AAINHTTRVF CGAEFSIPLP NDGRDMEDGS KYKSLFTCVD
AETMEVRWQA LIDGNCDLVA SSYDGKLAAT NQYNTEMGIH YEGMMGAEMD ACLFFNVARI
EEAVKAGKFT TIGSSKVPVV DGTRASNADP KTALTCYVPI PKNPHGVNIS PDGKYYACSG
KLSPTASVIE HALVLKWFDG ELPEAREAVV AEPEIGLGPL HTGFDNKGNA YTTLFLDSQI
VKWNVEAAIK SFKGDTAAKV VLDRIDVHYQ PGHGFTSMGE TKESDGRYFV SDNKFSKDRF
LPVGPLHAET AQLIDISGDK MKLVADHSVY SEPHDSIIIR RDIIKTRQIY NLDDFPHAVK
DPKESGVFRD GKKVTVKLTS QAPAFSLREF KVKKGDEVTI ILTNLDKVED LTHAMAIPKY
DINFIVNPQQ TQSVTFVADQ PGVYWCYCTN FCHALHLEMR SRMIVEA
//
