ID A0A2U9NZY9_STRAS Unreviewed; 908 AA.
AC A0A2U9NZY9;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=DMT42_09395 {ECO:0000313|EMBL:AWT42508.1};
OS Streptomyces actuosus.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1885 {ECO:0000313|EMBL:AWT42508.1, ECO:0000313|Proteomes:UP000247634};
RN [1] {ECO:0000313|EMBL:AWT42508.1, ECO:0000313|Proteomes:UP000247634}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25421 {ECO:0000313|EMBL:AWT42508.1,
RC ECO:0000313|Proteomes:UP000247634};
RA Liu W., Sun F., Hu Y.;
RT "The complete genome sequence of a nosiheptide producer Streptomyces
RT actuosus ATCC 25421: deducing the ability of producing a new class III
RT lantibiotics.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; CP029788; AWT42508.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U9NZY9; -.
DR KEGG; sact:DMT42_09395; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000247634; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000247634};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 16..278
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 326..497
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 664..871
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 908 AA; 98605 MW; EFC988BF6F3DE0E6 CRC64;
MAETASKKTD STPGAGRPRL MLMDGHSLAY RAFFALPAEN FTTATGQPTN AIYGFASMLA
NTLRDEAPTH FAVAFDVSRK TWRSEEFTEY KANRSKTPDE FKGQVELIGE LLDAMHAQRF
AVDGFEADDI IATLATQAEA EGFEVLIVTG DRDNFQLITD NVTVLYPTKG VSELTRFTPE
KVFEKYGLTP AQYPDFAALR GDPSDNLPGI PGVGEKTAAK WINQFGSFAE LVERVDEVKG
KAGQNLRDHL EAVKLNRRLT EMVRTVELDR TVADLERAPY DRKAVAMILD TLEIRNPSLR
ERLMAVDPGA EQAETTPVVA DGVTVDGTVL GTGELAPWLA EHAGEPLGVA TVDTWGLGTG
SVAEVALAAA GGAAAWFDPA QLDEADETAF AAWLADPARP KVFHNAKGAM RVFAEHGWSV
DGVTMDTALA AYLVKPGRRS FDLDALSMEY LHRELAPAAA ADGQLAFGAD DGAEAEALMI
QARAVLDLGQ AFESKLQEVG AADLLRDMEL PTSALLARME RHGIAADRTH LEAMEQMFAG
AVQQAVKEAH AAAGHEFNLG SPKQLQEVLF GELGLPKTKK TKTGYTTDAD ALAWLATQTD
NELPVIMLRH REQAKLRVTV EGLIKTIAAD GRIHTTFNQT VAATGRLSST DPNLQNIPVR
TDEGRAIRRG FVVGEGYESL MTADYSQIEL RVMAHLSEDA GLIEAFTSGE DLHTTAASQV
FGVEPAAVDA EMRRKIKAMS YGLAYGLSAF GLSQQLNIEA AEARALMDAY FDRFGGVRDY
LRRVVDEARA TGYTATIFGR RRYLPDLNSD NRQRREAAER MALNAPIQGT AADIVKIAML
KVDAALREAD LKSRMLLQVH DEIVLEIAPG EREKAEELVR REMSNAVDLS VPLGVSVGAG
PDWESAAH
//
