ID A0A2U9P4I2_STRAS Unreviewed; 825 AA.
AC A0A2U9P4I2;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=DMT42_18785 {ECO:0000313|EMBL:AWT44154.1};
OS Streptomyces actuosus.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1885 {ECO:0000313|EMBL:AWT44154.1, ECO:0000313|Proteomes:UP000247634};
RN [1] {ECO:0000313|EMBL:AWT44154.1, ECO:0000313|Proteomes:UP000247634}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25421 {ECO:0000313|EMBL:AWT44154.1,
RC ECO:0000313|Proteomes:UP000247634};
RA Liu W., Sun F., Hu Y.;
RT "The complete genome sequence of a nosiheptide producer Streptomyces
RT actuosus ATCC 25421: deducing the ability of producing a new class III
RT lantibiotics.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DnaX/STICHEL family.
CC {ECO:0000256|ARBA:ARBA00006360}.
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DR EMBL; CP029788; AWT44154.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U9P4I2; -.
DR KEGG; sact:DMT42_18785; -.
DR OrthoDB; 9810148at2; -.
DR Proteomes; UP000247634; Chromosome.
DR GO; GO:0009360; C:DNA polymerase III complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR CDD; cd00009; AAA; 1.
DR CDD; cd18137; HLD_clamp_pol_III_gamma_tau; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 1.20.272.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR008921; DNA_pol3_clamp-load_cplx_C.
DR InterPro; IPR022754; DNA_pol_III_gamma-3.
DR InterPro; IPR012763; DNA_pol_III_sug/sutau_N.
DR InterPro; IPR045085; HLD_clamp_pol_III_gamma_tau.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR02397; dnaX_nterm; 1.
DR PANTHER; PTHR11669:SF0; PROTEIN STICHEL; 1.
DR PANTHER; PTHR11669; REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT; 1.
DR Pfam; PF13177; DNA_pol3_delta2; 1.
DR Pfam; PF12169; DNA_pol3_gamma3; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF48019; post-AAA+ oligomerization domain-like; 1.
PE 3: Inferred from homology;
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022932};
KW Reference proteome {ECO:0000313|Proteomes:UP000247634};
KW Transferase {ECO:0000256|ARBA:ARBA00022932}.
FT DOMAIN 37..181
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 445..620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 700..807
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 502..518
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 569..583
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 597..615
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 765..781
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 825 AA; 82064 MW; 9E39E5B8973690F9 CRC64;
MSSLALYRRY RPESFAEVIG QEHVTDPLQQ ALRNNRVNHA YLFSGPRGCG KTTSARILAR
CLNCEQGPTP TPCGECQSCH DLARNGPGSI DVIEIDAASH GGVDDARELR EKAFFGPASS
RYKIYIIDEA HMVTSAGFNA LLKVVEEPPE HLKFIFATTE PEKVIGTIRS RTHHYPFRLV
PPGTLRDYLA EVCQKEKIPV EDGVLPLVVR AGAGSVRDSM SVMDQLLAGA GEDGVTYAMA
TALLGYTDGS LLDSVVEAFA SGDGAAAFEV VDRVIEGGND PRRFVADLLE RLRDLVILAA
VPDAAEKGLI DAPADVIERM QAQARSFGAA ELSRAADLVN EGLTEMRGAT SPRLQLELIC
ARVLLPAAYG DERSVMARLD RIERGVHFSA GTGLPAMGYV PGPEAHGAAP AAHGPAAGGF
APAAAGGAPV PPGGGAAAAR AAVRSSGGGA GAPGGDAGTG SGGVPAGGPG AGPGGVPGGA
PSGAPVGVPA APEGPGAGGT TVPAPAPAPA PAAAPTPAPV AEQPTAAAAP AAPGAWPTAA
PAGGGRRPGG WPTAAPAGGA ARPATGPGAP GAPGTPGTPA PSATPPASAG AGSARPQPTP
APAPAAAPSA PAPAPSGGLD PRSLWPNILE AVKNRRRFTW ILLSQNAQVT GFDGTTLQLG
FVNAGARDNF AGSGSEDVLR QALAEQFNVH WKIEAVVDPS GGSAPAPSGG FGGGGGYGGP
SGHGGSPGAS GYGGGVAAPA APRPAAPAPS APHGASAPTA APHASAPAPA SPAPQPAAPE
PSYAVAPEDD TPEDDDPDLN ESALSGHELI VRELGATVVE EFTNE
//
