ID A0A2V0P5B7_9CHLO Unreviewed; 1139 AA.
AC A0A2V0P5B7;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=Rsub_07547 {ECO:0000313|EMBL:GBF95046.1};
OS Raphidocelis subcapitata.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Sphaeropleales; Selenastraceae; Raphidocelis.
OX NCBI_TaxID=307507 {ECO:0000313|EMBL:GBF95046.1, ECO:0000313|Proteomes:UP000247498};
RN [1] {ECO:0000313|EMBL:GBF95046.1, ECO:0000313|Proteomes:UP000247498}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-35 {ECO:0000313|EMBL:GBF95046.1,
RC ECO:0000313|Proteomes:UP000247498};
RX PubMed=29795299; DOI=10.1038/s41598-018-26331-6;
RA Suzuki S., Yamaguchi H., Nakajima N., Kawachi M.;
RT "Raphidocelis subcapitata (=Pseudokirchneriella subcapitata) provides an
RT insight into genome evolution and environmental adaptations in the
RT Sphaeropleales.";
RL Sci. Rep. 8:8058-8058(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBF95046.1}.
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DR EMBL; BDRX01000059; GBF95046.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2V0P5B7; -.
DR STRING; 307507.A0A2V0P5B7; -.
DR InParanoid; A0A2V0P5B7; -.
DR Proteomes; UP000247498; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000247498}.
FT DOMAIN 97..409
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 427..904
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 742..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 781..848
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1040..1139
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1123..1139
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 118..125
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1139 AA; 117866 MW; 28461F8B8D6F97F3 CRC64;
MRAAPPPRPA AAAAAAPPLA PLAMRHRSGP TAPRAAACAG IAAMRAPPAC WRRPLHVAAA
AAPPSGGPAP APEGPRAAGP QLLMTPEVER SVQDFLATLS LEQRAAALCE AQRVRVIAGP
GSGKTRVVAA RIAELLRRGE RPGAVLAITF TNKAASELKE RLEALLGAGA GGLARLRVTT
FHALCAALLG RYNDTLPPEL VPPKFVIYDQ PDATSCLQRA LASDGGVPVT RYEARDTIAE
ISALKNHAPA VYSPYMGGGH RKAGGRDLLS SIRRAGAPPR RFAALKTAEE IRLAKRYDLY
NAQLAAEGAL DFDDLLHATV AMLTERPDIL DRCRAQWRHV LCDESQDTNQ AQFALLRLLL
GERNTLFAVG DPNQGIYGFR GSSHAHLTGA EASFGDVTTL YLRDNYRSQP PIVSAGESLL
TRSHPKPSLY RGANAVRAPQ RFGAAAAAAA AGAPCVHIWS VPDQYDENDV AIDIVKRAAE
EVDSLSEVAV LYRAHYMANM LEKRLRRERL PFARQGRKSL FESKEVATAA ALLRLAATPQ
DTAALLSVLN SGLPIAKGLG QATIGKVEAW CEEQAAAAAE AGGGARPPTL AGALLGDGWG
TPLEPRLGAT AGAALAADAM RLPWRFRERD DEAELAELES FWARIEALPN IPPPATAAAA
AAPLPARGKQ ARVVARLRGL VACARAVAAS EAPGRVLDCL LRASGFPFFL EAVESKAEKA
RAAAAARSGA AAAAAAAGRA RRAAASDADD GEEEGEEGGL ERRYSNVEDF RSLAPKAAAS
GLGADDLFDE DGEAGEAEEE EAAAAPRGDY GGWAGGGGGG GGGGGGGGGD FGSRGAVQAP
AGGASSGGGA ALHLEGPLSA ALPRGGGGLE ALAAFAEFTK LASGEDADDA VPKIQLMTIH
NAKGKEFGVT LLIGNKRDRD DDVPSERLQS KNLVYTGVTR AKDVLHIVYA RGQSEFKDGR
RVPVERPDRG GSSVGEGINP YVEHLVREVQ RGELPGAALH EMQRGEWPAE FVARRLSGAD
AHSAYGRSPL SRGAAAARLP PLAGGPVVAS ARAGAPGRRA SRRAGAGSRQ GAGGSAAGGA
SSGGDGEGEG GLELPPWLQD GAPAAAPPVV GSGKRRTPAA ARGARHPPPP PPPPLTRRR
//
