ID   A0A2V1INH1_9BACT        Unreviewed;       369 AA.
AC   A0A2V1INH1;
DT   12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT   12-SEP-2018, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   SubName: Full=2-iminoacetate synthase ThiH {ECO:0000313|EMBL:PWB01193.1};
GN   Name=thiH {ECO:0000313|EMBL:PWB01193.1};
GN   ORFNames=C5O23_10375 {ECO:0000313|EMBL:PWB01193.1};
OS   Duncaniella muris.
OC   Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae;
OC   Duncaniella.
OX   NCBI_TaxID=2094150 {ECO:0000313|EMBL:PWB01193.1, ECO:0000313|Proteomes:UP000244905};
RN   [1] {ECO:0000313|Proteomes:UP000244905}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 103720 {ECO:0000313|Proteomes:UP000244905};
RA   Clavel T., Strowig T.;
RL   Submitted (FEB-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PWB01193.1}.
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DR   EMBL; PUEC01000024; PWB01193.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2V1INH1; -.
DR   Proteomes; UP000244905; Unassembled WGS sequence.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0009228; P:thiamine biosynthetic process; IEA:InterPro.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR010722; BATS_dom.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR012726; ThiH.
DR   InterPro; IPR034428; ThiH/NoCL/HydG-like.
DR   NCBIfam; TIGR02351; thiH; 1.
DR   PANTHER; PTHR43583; 2-IMINOACETATE SYNTHASE; 1.
DR   PANTHER; PTHR43583:SF1; 2-IMINOACETATE SYNTHASE; 1.
DR   Pfam; PF06968; BATS; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDF00301; 2-iminoacetate_synthase_(ThiH); 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SMART; SM00876; BATS; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
PE   4: Predicted;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000244905};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          256..359
FT                   /note="Biotin and thiamin synthesis-associated"
FT                   /evidence="ECO:0000259|SMART:SM00876"
SQ   SEQUENCE   369 AA;  42383 MW;  1B499BE94C9B75D0 CRC64;
     MFSEELKKYD WDETTRFIQS RTAAHVESAL RKQHLSVEDF MALISPAAEP YLEQMAQLSH
     RYTMERFGKT ISLYIPLYVS NACTNSCVYC GFNHNNPLER VTLTLDQVKA ECEAIRRLGP
     FENLLIVSGE FPALNGVDYL EKVLKVARPY FNNLTIEVMP MKTESYARLV ESGLNGVVCF
     QETYNAANYK TYHPKGMKSI FDWRVNGFDR MGQAGVHKIG MGVLIGLEDW RTDLAMLARH
     LRYLRKNYWR TRYSVNFPRM CPAEGGYQPK VVMSDRELAQ VTFAFRIFDH DVDISYSTRE
     DPEFRANMMK LGVTSMSAGS KTEPGGYCST PDALEQFEVS DPRSPEAVAA DIRRLGYEAV
     WKDWDKIFD
//