ID A0A2V3DQF7_9MICC Unreviewed; 712 AA.
AC A0A2V3DQF7;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=CVS29_15715 {ECO:0000313|EMBL:PXA64374.1};
OS Arthrobacter psychrochitiniphilus.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Micrococcaceae;
OC Arthrobacter.
OX NCBI_TaxID=291045 {ECO:0000313|EMBL:PXA64374.1, ECO:0000313|Proteomes:UP000246303};
RN [1] {ECO:0000313|EMBL:PXA64374.1, ECO:0000313|Proteomes:UP000246303}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GP3 {ECO:0000313|EMBL:PXA64374.1,
RC ECO:0000313|Proteomes:UP000246303};
RA Liu Q., Xin Y.-H.;
RT "Genetic diversity of glacier-inhabiting Cryobacterium bacteria in China
RT and description of Cryobacterium mengkeensis sp. nov. and Arthrobacter
RT glacialis sp. nov.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PXA64374.1}.
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DR EMBL; QHLZ01000012; PXA64374.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2V3DQF7; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000246303; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF1; BETA-GALACTOSIDASE GANA; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 2.
DR PIRSF; PIRSF001084; B-galactosidase; 2.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Reference proteome {ECO:0000313|Proteomes:UP000246303}.
FT DOMAIN 10..379
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 390..514
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT DOMAIN 564..630
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 146
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 302
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 107
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 145
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 310
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 712 AA; 76940 MW; 941885F6A46997AE CRC64;
MIPAIAYGGD YNPEQWTAET VDRDIELMHE AGVNLVSVGI FSWAVLQPTP GVFDFGWLDT
VMDKLHDAGI AVDLATATAS PPPWLGHLHP DTLPVTRDGV RLSYGSRQQF NPSSATFRRE
SLIMVRAIAE RYAKHPALKM WHVNNEYGCH VHESFDEESA TAFREWLQAR YGTLAELNRV
WGTTFWSQRY SDWAQVSAPR AMPTLPNPTM MTDWNRFCSD ALLACYTAEK EVLNELTPDV
PLTTNFMGAF AWVDGWKWGT EIDIISNDSY PDPSDPRAGR EFAFESDLMR SLGGGKPFIQ
LEQTTSAVQW RPRNAVKRPG QYKLWSLQAV ARGADGICQF QWRQSASGAE TFHSGMVPHA
GKASRTWHEV VELGSALKDI GVVAGSRVQA QAAIVWDWES AWAQRNSIGP VDTEPLTIAK
SWHASLFEMG IATDFVHPDH DLGKYQLIIV PSLFAVSDAF AAALARAAAN GAQVIVSCLT
GALNAEGHAQ LGGYLGPLAP MLGVRVLDFS PLGVVPHTTE IEVNGPLDGP ALDPIYEPIS
SLIRTPATER SVALLPTAAD GITGNAIGWV ENLEVIEGAG ACVLSTFAGL DTSGWPAITR
RQHGTGTAWY VASNPDARGR SEVISAVVRE SAGSAHPIRP VLDAPVPGIE AVLRGQVLFL
LNHADTPALV PGISGTVLYD GGQPSPEGPR RGTVLSGADV SVGPRNAVVL QL
//
