ID A0A2V4AFM2_9BACT Unreviewed; 799 AA.
AC A0A2V4AFM2;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00012555};
DE EC=2.4.1.129 {ECO:0000256|ARBA:ARBA00012555};
GN ORFNames=DF185_02020 {ECO:0000313|EMBL:PXY02894.1};
OS Marinifilum breve.
OC Bacteria; Bacteroidota; Bacteroidia; Marinilabiliales; Marinifilaceae.
OX NCBI_TaxID=2184082 {ECO:0000313|EMBL:PXY02894.1, ECO:0000313|Proteomes:UP000248079};
RN [1] {ECO:0000313|EMBL:PXY02894.1, ECO:0000313|Proteomes:UP000248079}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JC075 {ECO:0000313|EMBL:PXY02894.1,
RC ECO:0000313|Proteomes:UP000248079};
RA Fu T.;
RT "Marinifilum breve JC075T sp. nov., a marine bacterium isolated from Yongle
RT Blue Hole in the South China Sea.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004752}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PXY02894.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; QFLI01000001; PXY02894.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2V4AFM2; -.
DR OrthoDB; 9766909at2; -.
DR Proteomes; UP000248079; Unassembled WGS sequence.
DR GO; GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF11; PENICILLIN-BINDING PROTEIN 1B; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670}.
FT DOMAIN 85..264
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 438..687
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 768..787
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 799 AA; 90719 MW; 07718B697637CADB CRC64;
MAKTKKTTSR KTNSKRKTSS KKKTKKRSIK SYIIRLGLLA IACALVGIGL LFSTVYLGFW
GKLPDYYTLR NIKNADASEI YSEDGAILGR VYAENRTNVN FKDISPNVIN SLIATEDVRF
YEHQGVDQRS MLRVLVKSLI MRDRSSGGGS TLSQQLAKNL YPRKRYGAIS MPINKIREAI
TASRLEKIYS KKEILQLYLN TVSFGENVFG IESASRQFFS KPASALKTQE CAVLIGMLKA
PTYYNPRLHP DRSKQRRNVV LNQMAKYEYI SETKAKQLKA LPLGLRYNNQ ASKNGLAPYL
RENIRIQTNK ILADYPKEDG SYYDIYRDGL KITTTLDSKL QAYAEQSVGE HLKRLQDLFY
KHWGTSKPWD NKKDILSDAK RRSDRYKKLK KSGKSEQEIN KIFNKKVDMK IFTWSGEKNV
NMSPNDSIKH YVQLLNTGFL AMEPHSGKIK AWVGGIDFNH FKYDHVTAKR QVGSVFKPVV
FSAALEEGVS PFEYLPNERK VYEEYDNWSP RNADNKYEGS YSMEGALAES VNTIAVDVLL
QTGIRDAIVL AEEMGVDSNL PKVPSLALGT ANISLFEMIQ VYATLANRGV HVDPYYLVKI
EDKNGNLIAD LSKQRGENRF VLSPQNADII NHMLQAVVNS GTGKSLRNVY KLEGDLAGKT
GTTNSHADGW YIGYNSNLVA GAWVGADDMR VHFRSLTLGQ GASMALPIYG KFMHKLSRNR
KFTNYSYASI HKPDNDILAQ LSIPHYMPKD SGLLKNIFGV KAKDRDKLME RKQRKEKRKA
KRKSIYQRIK NIFKKKNKD
//
