ID   A0A2W2LG81_9ACTN        Unreviewed;       390 AA.
AC   A0A2W2LG81;
DT   12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT   12-SEP-2018, sequence version 1.
DT   24-JAN-2024, entry version 17.
DE   SubName: Full=Class E sortase {ECO:0000313|EMBL:PZH06867.1};
GN   ORFNames=C1I97_17830 {ECO:0000313|EMBL:PZH06867.1};
OS   Streptomyces sp. NTH33.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1735453 {ECO:0000313|EMBL:PZH06867.1, ECO:0000313|Proteomes:UP000248559};
RN   [1] {ECO:0000313|EMBL:PZH06867.1, ECO:0000313|Proteomes:UP000248559}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NTH33 {ECO:0000313|EMBL:PZH06867.1,
RC   ECO:0000313|Proteomes:UP000248559};
RA   Sahin N., Ay H., Saygin H.;
RT   "Draft genome sequence of Streptomyces sp. NTH33.";
RL   Submitted (JAN-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PZH06867.1}.
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DR   EMBL; POTZ01000171; PZH06867.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2W2LG81; -.
DR   OrthoDB; 5242879at2; -.
DR   Proteomes; UP000248559; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   CDD; cd05830; Sortase_E; 1.
DR   Gene3D; 2.40.260.10; Sortase; 1.
DR   InterPro; IPR005754; Sortase.
DR   InterPro; IPR023365; Sortase_dom-sf.
DR   InterPro; IPR042003; Sortase_E.
DR   NCBIfam; NF033747; class_E_sortase; 1.
DR   NCBIfam; TIGR01076; sortase_fam; 1.
DR   Pfam; PF04203; Sortase; 1.
DR   SUPFAM; SSF63817; Sortase; 1.
PE   4: Predicted;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000248559};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        177..199
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   REGION          1..170
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        289
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605754-1"
FT   ACT_SITE        358
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605754-1"
SQ   SEQUENCE   390 AA;  41421 MW;  5C89E6ABF8D41C18 CRC64;
     MTALRPERES DPSYGQQPYE GSGTFGEWYG VGAQGPPQEP YAAPYAAPVV PQAGPSGPAG
     VPYEPAGDAA TAYAPPAPHP GPHPGPPAVD EETMALRIPD PPPEASSATG TTEPPTGGRA
     ARRKAARRRH GRRGAVGEAV PEGGSPAERP SAPLSRVEAR RQARARRASP GVVASRIIGE
     VFITTGVVML LFVTYQLWWT NVRAHAQAGS EANQLQNEWA SGKRNPGAFE PGQGFALLHI
     PKLDVVVPVA EGISKSKVLD KGMVGHYAEG ALNTAMPEAK TGNFGLAGHR NTHGEPFRYI
     NRLSPGDAIV VETQDTYFVY KMASMLPVTP PANTSVLDPV PKGSGFTKPG RYITLTTCTP
     EFTSKYRLIV WGKMVEERPR SKGKPDALVS
//