ID A0A2X0KAM6_9ACTN Unreviewed; 669 AA.
AC A0A2X0KAM6;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00012555};
DE EC=2.4.1.129 {ECO:0000256|ARBA:ARBA00012555};
GN ORFNames=DN069_18800 {ECO:0000313|EMBL:RAG84130.1};
OS Streptacidiphilus pinicola.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptacidiphilus.
OX NCBI_TaxID=2219663 {ECO:0000313|EMBL:RAG84130.1, ECO:0000313|Proteomes:UP000248889};
RN [1] {ECO:0000313|EMBL:RAG84130.1, ECO:0000313|Proteomes:UP000248889}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MMS16-CNU450 {ECO:0000313|EMBL:RAG84130.1,
RC ECO:0000313|Proteomes:UP000248889};
RA Roh S.G., Park S., Kim M.-K., Yun B.-R., Park J., Kim M.J., Kim Y.S.,
RA Kim S.B.;
RT "Streptacidiphilus pinicola sp. nov., isolated from pine grove soil.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAG84130.1}.
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DR EMBL; QKYN01000072; RAG84130.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2X0KAM6; -.
DR OrthoDB; 8865355at2; -.
DR Proteomes; UP000248889; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF34; PENICILLIN-BINDING PROTEIN 1A; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000248889};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 44..65
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 89..264
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 443..629
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
SQ SEQUENCE 669 AA; 71981 MW; 0A56E96BAE2E84D5 CRC64;
MGPLGRRLGP VGRWLLRVGR RLAWEYPRPG RVGWRRWMPS WRQWLGGAAY LIIALVATTG
IAFAMTDIPK NLNTFATQQV NVYYWADGTE MARTGPVDRQ AVPLGQIPKP VQWDILAAEN
ETFYSDDGVS PRGMMRAVYE MIKGGDTQGG STITQQYVKN VYLNQQQTVT RKLTEAFIAI
KLDSKLSKDQ ILDGYLNTSW FGRGTYGIER AARAYYGVDV SQLNVSQGAF LASLLKGAGM
YDPAISPDNQ QRAVTRWKWI LDRMVVIHQL TPAQRAQYTT FPEPIQPRLQ GGLTGQTGYL
VDLATQYVTA HSSVDPRLFD LGGYQIYTTF QKRRVTALAA SVKAASKGLD PAKRPADKDV
SFGAATVAAD GRILAVYGGP DYVKQGFDDA NTATVPVGTA FTPLVYAAGL TDGVQLQPGG
SRTPVSGTSV YDGDNKMPVQ TPEGPYWNKD GQLVKINNDG GRSFGKVTLH QAVVDSINGP
MAQLGMDVGL TQLRGTVEKF GLLPASMGEP VPVFPLGNST PSAIRMADAY GAFPGEGRHV
EPYSVSRLLF SGHDVGVDRP QPVQVMTADV AHEVDSALTE SVASGAAQGA RLPGVEVAGK
AGTMLDATSG WFIGYTGQSV TAVTMFHADP NNGLMLPLTG VMGAPAAQPT STVPTRIFAR
YTRATASLG
//
