ID A0A2Y9C4B5_9FIRM Unreviewed; 536 AA.
AC A0A2Y9C4B5;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=A8806_101586 {ECO:0000313|EMBL:PWJ32298.1},
GN SAMN05216536_101586 {ECO:0000313|EMBL:SSA54132.1};
OS Faecalicatena orotica.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Faecalicatena.
OX NCBI_TaxID=1544 {ECO:0000313|EMBL:SSA54132.1, ECO:0000313|Proteomes:UP000251306};
RN [1] {ECO:0000313|EMBL:SSA54132.1, ECO:0000313|Proteomes:UP000251306}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NLAE-zl-C242 {ECO:0000313|EMBL:SSA54132.1,
RC ECO:0000313|Proteomes:UP000251306};
RA Cai Z.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:PWJ32298.1, ECO:0000313|Proteomes:UP000245845}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NLAE-zl-C242 {ECO:0000313|EMBL:PWJ32298.1,
RC ECO:0000313|Proteomes:UP000245845};
RA Kelly W.;
RT "The Hungate 1000. A catalogue of reference genomes from the rumen
RT microbiome.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; QGDL01000001; PWJ32298.1; -; Genomic_DNA.
DR EMBL; UETE01000001; SSA54132.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2Y9C4B5; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000245845; Unassembled WGS sequence.
DR Proteomes; UP000251306; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000245845};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 14..180
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 194..502
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 506..533
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 536 AA; 60116 MW; AD69A4ED24BECCE4 CRC64;
MAIDNKKEQE RETLHRAIWA IADELRGAVD GWDFKNYVLG TMFYRYISEN LAAYVNQGEI
EAGNTDFDYA KMHDDEAKEA KEGLVEEKGF FIPPSELFCN VHKNCSNDKA VFIDCEGKPK
NVKENLNVFL EMIFQNIEES AKGSESEGSF AGLFDDFDVN SNKLGSTVAK RNERLVKLLN
GVADMNLGSV KDHDIDAFGD AYEYLMTMYA SNAGKSGGEF FTPADVSELL TRLGTVGKTS
VNKVYDPACG SGSLLLKAEK ILGKDAVKTG FYGQEINITT YNLCRINMFL HDIGFDKFDI
ECDDTLTNPQ HWDDEPFELI VSNPPYSIKW AGDENPLLIN DPRFSPAGVL APKSKADLAF
IMHSLSWLAS NGTATIVCFP GIMYRGGAEQ KIRKYLIDNN YVDCVIQLPS NLFFGTSIAT
CIMVMKKNKV DNKTLFIDAS NECIKVTNNN KLTPDNINRI VDVFSSREDI PHFSHLASYN
EIAENSFNLS VSTYVEVEDT REKIDINELN AEIKRIVARE QELRDEIDKI IAEIEV
//
