ID A0A2Y9QGJ7_TRIMA Unreviewed; 4603 AA.
AC A0A2Y9QGJ7;
DT 12-SEP-2018, integrated into UniProtKB/TrEMBL.
DT 12-SEP-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=RCR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012249};
DE EC=2.3.2.33 {ECO:0000256|ARBA:ARBA00012249};
GN Name=LOC101355836 {ECO:0000313|RefSeq:XP_023582480.1};
OS Trichechus manatus latirostris (Florida manatee).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Afrotheria; Sirenia; Trichechidae; Trichechus.
OX NCBI_TaxID=127582 {ECO:0000313|Proteomes:UP000248480, ECO:0000313|RefSeq:XP_023582480.1};
RN [1] {ECO:0000313|RefSeq:XP_023582480.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine.;
CC EC=2.3.2.33; Evidence={ECO:0000256|ARBA:ARBA00000333};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBCELLULAR LOCATION: Cell projection, axon
CC {ECO:0000256|ARBA:ARBA00004489}.
CC -!- SIMILARITY: Belongs to the RING-Cys relay (RCR) family.
CC {ECO:0000256|ARBA:ARBA00005415}.
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DR RefSeq; XP_023582480.1; XM_023726712.1.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000248480; Unplaced.
DR GO; GO:0030424; C:axon; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd19799; Bbox2_MYCBP2; 1.
DR CDD; cd16463; RING-H2_PHR; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 2.60.120.820; PHR domain; 2.
DR Gene3D; 2.130.10.30; Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II; 2.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR004939; APC_su10/DOC_dom.
DR InterPro; IPR017868; Filamin/ABP280_repeat-like.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR012983; PHR.
DR InterPro; IPR038648; PHR_sf.
DR InterPro; IPR009091; RCC1/BLIP-II.
DR InterPro; IPR000408; Reg_chr_condens.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR45943; E3 UBIQUITIN-PROTEIN LIGASE MYCBP2; 1.
DR PANTHER; PTHR45943:SF1; E3 UBIQUITIN-PROTEIN LIGASE MYCBP2; 1.
DR Pfam; PF03256; ANAPC10; 1.
DR Pfam; PF08005; PHR; 2.
DR Pfam; PF00415; RCC1; 1.
DR Pfam; PF13540; RCC1_2; 1.
DR SMART; SM01337; APC10; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF50985; RCC1/BLIP-II; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51284; DOC; 1.
DR PROSITE; PS50194; FILAMIN_REPEAT; 1.
DR PROSITE; PS00626; RCC1_2; 2.
DR PROSITE; PS50012; RCC1_3; 3.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000248480};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT REPEAT 601..656
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 959..1009
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 1010..1067
FT /note="RCC1"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00235"
FT REPEAT 2341..2434
FT /note="Filamin"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00087"
FT DOMAIN 3643..3821
FT /note="DOC"
FT /evidence="ECO:0000259|PROSITE:PS51284"
FT DOMAIN 4353..4404
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 87..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 170..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 898..928
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2634..2854
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2991..3010
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3530..3554
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3841..3861
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 102..121
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 908..924
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2639..2660
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2661..2682
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2708..2775
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2785..2815
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2826..2848
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3844..3858
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4603 AA; 505260 MW; 8FC83ED1F0714748 CRC64;
MMMCSATASP AVVSSGPGGD GFFPAATISS SPAPGAPFMP VSEGSGAAAG LGLGLPAADS
RGNYQLLLSG RALADRYRRI YTAALSDRDQ GGSSAGHPAS RNKKILNKKK LKRKQKSKSK
VKTRSKSENL ENTVIIPDIK LHSNPSAFNI YCNVRHCVLE WQKKETSLAA ASKNSVQSGE
SDSDEEEESK EPPIKLPKII EVGLCEVFEL IKETRFSHPS LCLRSLQALL NVLQGQQPEG
LQSEPPEVLE SLFQLLLEIT VRSTGMNDST GQSLTALSCA CLFSLVASWG ETGRTLQAIS
AILTNNGSHA CQTIQVPTIL NSLQRSVQAV LVGKVQIQDW FSNGIKKAAL MHKWPLKEIS
VDEDDQCLLQ NDGFFLYLLC KDGLYKIGSG YSGTVRGHIY NSTSRIRNRK EKKSWLGYAQ
GYLLYRDVSN HSMTAIRISP ETLEQDGTVM LPDCHTEGQN ILFTDGEYIN QIAASRDDGF
VVRIFATSTE PVLQQELQLK LARKCLHACG ISLFDLEKDL HIISTGFDEE SAILGAGREF
ALMKTASGKI YYTGKYQSLG IKQGGPSAGK WVELPITKSP KIVHFSVGHD GSHALLVAED
GSIFFTGSAS KGEDGESTKS RRQSKPYKPK KIIKMEGKIV VYTACNNGSS SVISKDGELY
MFGKDAIYSD SSSLVTDLKG HFVIQVAMGK AHTCVLMKNG EVWTFGVNNK GQCGRDTGAM
NQGGKGFGVE NMATAMDEDL EEELDEKDEK SMMCPPGMHK WKLEQCMVCT VCGDCTGYGA
SCVSSGRPDR VPGGICGCGS GESGCAVCGC CKACARELDG QEARQRGILD AVKEMIPLDL
LLAVPVPGVN IEEHLQLRQE EKRQRVIRRH RLEEGRGPLV FAGPIFMNHR EQALARLRSH
PAQLKHKRDK HKDGSGERGE KDASKITTYP PGSVRFDCEL RAVQVSCGFH HSVVLMENGD
VYTFGYGQHG QLGHGDVNSR GCPTLVQALP GPSTQVTAGS NHTAVLLMDG QVFTFGSFSK
GQLGRPILDV PYWNAKPAPM PNIGSKYGRK ATWIGASGDQ TFLRIDEALI NSHVLATSEI
FASKHIIGLV PASISEPPPF KCLLINKVDG SCKTFNDSEQ EDLQGFGVCL DPVYDVIWRF
RPNTRELWCY NAVVADARLP SAADMQSRCS ILSPELALPT GSRALTTRSH AALHILGCLD
TLAAMQDLKM GVASTEEETQ AVMKVYSKED YSVVNRFESH GGGWGYSAHS VEAIRFSSDT
DILLGGLGLF GGRGEYTAKI KLFELGPDGG DHETDGDLLA ETDVLAYDCA AREKYAMMFD
EPVLLQAGWW YVAWARVSGP SSDCGSHGQA SITTDDGVVF QFKSSKKSNN GTDVNAGQIP
QLLYRLPTSD GSASKGKQQT SEPVHILKRS FARTVSVECF ESLLSILHWS WTTLVLGVEE
LRGLKGFQFT ATLLDLERLR FVGTCCLRLL RVYTCEIYPV SATGKAVVEE TSKLAECIGK
TRTLLRKILS EGVDHCMVKL DNDPQGYLSQ PLSLLEAVLQ ECHNTFTACF HSFYPTPALQ
WACLCDLLNC LDQDIQEANF KTSSSRLLAA VMSALCHTSV KLTSIFPIAY DGEVLLRSIV
KQVSTENDST LVHRFPLLVA HMEKLSQSEE NISGMTSFRE VLEKMLVIVV LPVRNSLRRE
NELFSSLLVS NTCGLLASIV SELTASALGS EVDALNSLHS VKASANRFTK TSQGRSWNTG
NGSPDAICFS VDKPGIVVVG FSVYGGGGIH EYELEVLVDD SEHVGDSTHS HRWTSLELVK
GTYTTDDSPS DIAEIRLDKV VPLKENVKYA VRLRNYGSRT ANGDGGMTTV QCPDGVTFTF
STCSLSSNGT NQTRGQIPQI LYYRSEFDGD LQSQLLSKAN EEDKNCSRAL SVVSTVVRAA
KDLLHRALAV DADDIPELLS SSSLFSMLLP LIIAYIGPVA AAIPKVAVEV FGLVQQLLPS
VAILNQKYAP PAFNPNQSTD STTGNQPEQG LSACTTSNHY AVIESEHPYK PACVMHYKVT
FPECVRWMTI EFDPQCGTAQ SEDVLRLLIP VRTVQNSGYG PKLTSVHENL NSWIELKKFA
GSSGWPSMVL VLPGNEALFS LETASDYVKD DKASFYGFKC FAIGYEFSPG PDEGVIQLEK
ELANLGGVCA AALMKKDLAL PIGNELEEDL EILEEAALQV CKTHSGILGK GLALSHSPTI
LEALEGNLPL QIQSNEQSFL DDFIACVPGS SGGRLARWLQ PDSYADPQKT SLILNKDDIR
CGWPTTITVQ TKDQYGDVVH VPNMKVEVKA VPVSQKKTSL QQEQVKKSQR IPGSPAVTAA
SSNTDMTFGG LASPKLDVSY EPMIVKEARY IAITMMKVYE NYSFEELRFA SPTPKRPSEN
MLIRVNNDGS YCANWTPGAI GLYTIHVTID GIEIDAGLEV KVKDPPKGMV PPGTQLVKPK
AEPQPNKVRK FVAKDSAGLR IRSHPSLQSE QIGIVKVNGT ITFIDEIHND DGVWLRLNDE
TIKKYVPNMN GYTEAWCLSF NQHLGKSLLV PVDVTNSEGT WVQLDKNSMV EFCESDEGEA
WSLARDRGGN QYLRHEDEQV LLDQNSQTPP PSPFSVQAFN KGASCSAQGF DYGLGNNKGD
RGNVSTSSRP VSTSGKSELS SKHSRSLKPD GRMSRTATDQ KKPRGTEGLS ASESLMLKSD
AAKLRSDSHS RSLSPNHNTL QTLKSEGRMS SSLRAESPGP GSRSSSPKPK TLPASRSSPP
GAGSPRSSSP HDKNLPQKSA APVKTKLDPP RERSKSDSYT LDPDTLRKKK MPLTEPLRGR
STSPKPKSVP KDSKGSPGSE NRAPSPHVVQ ENLHSEVVEV CTSSTLKTNS LTDSTCDESS
EFKSVDEGSN KVHFSIGKAP LKDEQEMRAS PKISRKCANR HTRLKKEKSS FLFKGDGSKP
LEPAKQAMSP SVAECARAVF ASFLWHEGIV HDAMACSSFL KFNPELSKEH APIRSSLNSQ
QPTEEKETKL KNRHSLEISS ALNMFNIAPH GPDISKMGSI NKNKVLSMLK EPPLHEKCED
GKAETTFEMS MHHTMKSKSP LPLTLQHLVA FWEDISLATI KAASQNMIFP SPGSCAVLKK
KECEKENKKA KKEKKKKEKA EVRPRGNLFG EMAQLAVGGP EKDTVCELCG ESHPYPVTYH
MRQAHPGCGR YAGGQGYNSI GHFCGGWAGN CGDGGIGGST WYLVCDRCRE KYLREKQAAA
REKVKQSRRK PMQVKTPRAL PTMEAHQVIK ANALFLLSLS SAAEPSILCY HPTKPFPSQL
PSVKEGISED LPVKMPCLYL QTLARHHHEN LVGYQDDNLF QDEMRYLRST SVPAPYISVT
PDASPNVFEE PESNMKSMPP SLETSPITDT DLAKRTVFQR SYSVVASEYD KQHSILPARV
KAIPRRRVNS GDTEVGSSLL RHPSPELSRL ISAHSSLSKG ERNFQWPVLA FVIQHHDLEG
LEIAMKQALR KSACRVFAME AFNWLLCNVI QTTSLHDILW HFVASLTPAP VEPEEEEDEE
NKTNKENTEQ EKDTRVCEHP LSDIVIAGEA AHPLPHTFHR LLQTISDLMM SLPGGSSLQQ
MALRCWSLKF KQSDHQFLHQ SNVFHHINNI LSKSDDGDSE ESFSISIQSG FEAMSQELCI
VMCLKDLTSI VDIKTSSRPA MIGSLTDGST ETFWESGDED KNKTKNITIN CVKGINARYV
SVHVDNSRDL GNKVTSMTFL TGKAVEDLCR IKQVDLDSRH IGWVTSELPG GDNHIIKIEL
KGPENTLRVR QVKVLGWKDG ESTKIAGQIS ASVAQQRNCE AETLRVFRLI TSQVFGKLIS
GDAEPTPEQE EKALLSSPEG EEKVYNATSD ADLKEHMVGI IFSRSKLTNL QKQVCAHIVQ
AIRMEATRVR EEWEHAISSK ENANSQPNDE DASSDAYCFE LLSMVLALSG SNVGRQYLAQ
QLTLLQDLFS LLHTASPRVQ RQVTSLLRRV LPEVTPSRLA SIIGVKSLPP ADISDIIHST
EKGDWNKLGI LDMFLGCIAK ALTVQLKAKG TTITGTAGTT AGKGVTTVTL PMIFNSSYIR
RGESHWWLKG STPTQISEII IKLIKDMAAG HLSEAWSRVT KNAIAETIIA LTKMEEEFRS
PVRCIATTRL WLALASLCVL DQDHVDRLSS GRWMGKDGQQ KQMPMCDNHD DGETAAIILC
NICGNLCTDC DRFLHLHRRT KTHHRQVFKE EEEAIKVDLH EGCGRTKLFW LMALADSKTM
KAMVEFREHT GKPTTSSSEA CRFCGSRSGT ELSAVGSVCS DADCQEYAKI ACSKTHPCGH
PCGGVKNEEH CLPCLHGCDK HATTLKQDAD DMCMICFTEA LSAAPAIQLD CSHIFHLQCC
RRVLENRWLG PRITFGFISC PICKNKINHI VLKDLLDPIK ELYEDVRRKA LMRLEYEGLH
KSEAITTPGV RFYNDPAGYA MNRYAYYVCY KCRKAYFGGE ARCDAEAGQG DDYDPRELIC
GACSDVSRAQ MCPKHGTDFL EYKCRYCCSV AVFFCFGTTH FCNACHDDFQ RMTSIPKEEL
PHCPAGPKGK QLEGTECPLH VVHPPTGEEF ALGCGVCRNA HTF
//
