ID A0A2Z3I2D1_9GAMM Unreviewed; 924 AA.
AC A0A2Z3I2D1;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=DKL61_15250 {ECO:0000313|EMBL:AWM81582.1};
OS Gammaproteobacteria bacterium ESL0073.
OC Bacteria; Pseudomonadota; Gammaproteobacteria.
OX NCBI_TaxID=2070539 {ECO:0000313|EMBL:AWM81582.1, ECO:0000313|Proteomes:UP000246096};
RN [1] {ECO:0000313|EMBL:AWM81582.1, ECO:0000313|Proteomes:UP000246096}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ESL0073 {ECO:0000313|EMBL:AWM81582.1,
RC ECO:0000313|Proteomes:UP000246096};
RA Ellegaard K.M.;
RT "Reference genomes for bee gut microbiota database.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; CP029478; AWM81582.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2Z3I2D1; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000246096; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022839};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000246096};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 4..266
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 325..512
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 682..888
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 924 AA; 102286 MW; 4A643C6E78C12EC0 CRC64;
MSQAPLVLVD GSSYLYRAYH ALPPLATANG QPTGAIKGVI NMLKSLVKQY PDSPIAVVFD
AKGGSFRDEL FADYKAHRPP MPDDLRSQIE PLHQCVKAMG FPLLCVDRVE ADDVIGTLAI
ATAKTERSVV ISTGDKDMAQ LVNHKITLVN TMSGTTLDPQ GVVDKFGVEP KFIIDFLALM
GDKSDNIPGV PSVGEKTAVG LIQGLQGGLD TIYSHLDQIP NLPIRGAKSL PAKLEEHKEM
AYLSYQLATI KTDVELDITI DELNRSEEDA TTLIELYRTL EFKQWLDALL RKAPKQEQAK
MQQGDIFSNE EITPIETETS NTRDYQMITD TATLDAWVEK LNKAELIAFD TETTSVDAQQ
AELVGLSFSI KPHQAIYIPV AHDYMGAPKQ LSRDDVVAKL KPILENPNKA KVGQHAKYDI
NVLSHYNIDV QGVKYDTMME SYILDATGSR HDMDTLANNY LNEQTVKFED IAGKGAKQLT
FNQISLEQAA PYAAEDADIT LRLHETLWAK LQQEPNQAEV LTHIEIPLIP ALARIERNGA
LVDAKLLAAQ SQELGEKMIA LENKTFELAG QTFNMGSPKQ LGEILYTKLG LPVISKTATG
QPSTAEAVLA ELASQGYELP EVLMEYRSLS KLKSTYTDRL PEQINPRTGR IHTSYHQAVT
ATGRLSSSDP NLQNIPIRTP EGRRIRQAFI APKGYKLLAV DYSQIELRIM AHLAQDKGLL
DAFKHGLDVH KATAAEVFGV SVNQVTADQR RKAKAINFGL IYGMSAFGLA KQIGVDRKEA
QEYIDKYFTQ YPGVLSYMER TRELAAEKGY VETLFKRRLY LPDIKASNAN TRRGAERTAI
NAPMQGTAAD IIKRAMISVD AWLQHEKTDA KVILQVHDEL VLEVREDLTQ LVSDKIKILM
AEAAELSVPL VVDVGIGNNW DEAH
//
