ID A0A2Z4JPZ7_9BURK Unreviewed; 220 AA.
AC A0A2Z4JPZ7;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE SubName: Full=Site-2 protease family protein {ECO:0000313|EMBL:AWW50634.1};
GN ORFNames=DP176_03385 {ECO:0000313|EMBL:RAZ43062.1}, G6650_07780
GN {ECO:0000313|EMBL:MBT8619011.1}, G6684_06645
GN {ECO:0000313|EMBL:QWD27948.1}, Pas1_02215
GN {ECO:0000313|EMBL:AWW50634.1};
OS Polynucleobacter paneuropaeus.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Polynucleobacter.
OX NCBI_TaxID=2527775 {ECO:0000313|EMBL:AWW50634.1, ECO:0000313|Proteomes:UP000248592};
RN [1] {ECO:0000313|Proteomes:UP000248592}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MG-25-Pas1-D2 {ECO:0000313|Proteomes:UP000248592};
RA Hahn M.W.;
RT "Description of a new Polynucleobacter species.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:RAZ43062.1, ECO:0000313|Proteomes:UP000251072}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FUKU-NW-11 {ECO:0000313|EMBL:RAZ43062.1,
RC ECO:0000313|Proteomes:UP000251072};
RA Hahn M.W.;
RT "Genome of strain Polynucleobacter sp. FUKU-NW-11.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AWW50634.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=MG-25-Pas1-D2 {ECO:0000313|EMBL:AWW50634.1};
RX PubMed=30465643; DOI=10.1099/ijsem.0.003130;
RA Hoetzinger M., Schmidt J., Pitt A., Koll U., Lang E., Hahn M.W.;
RT "Polynucleobacter paneuropaeus sp. nov., characterized by six strains
RT isolated from freshwater lakes located along a 3000 km north-south cross-
RT section across Europe.";
RL Int. J. Syst. Evol. Microbiol. 69:203-213(2019).
RN [4] {ECO:0000313|EMBL:QWD27948.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AP-SiebGG-20-E3 {ECO:0000313|EMBL:QWD27948.1};
RA Hahn M.W., Hoetzinger M.;
RT "Genomes of Polynucleobacter paneuropaeus strains.";
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:MBT8619011.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AP-ClouGG-20-C5 {ECO:0000313|EMBL:MBT8619011.1};
RX PubMed=33674852; DOI=10.1093/gbe/evab019;
RA Hoetzinger M., Pitt A., Huemer A., Hahn M.W.;
RT "Continental-Scale Gene Flow Prevents Allopatric Divergence of Pelagic
RT Freshwater Bacteria. .";
RL Genome Biol. Evol. 13:evab019-evab019(2021).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the peptidase M50B family.
CC {ECO:0000256|ARBA:ARBA00007931}.
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DR EMBL; CP030085; AWW50634.1; -; Genomic_DNA.
DR EMBL; JAANGZ010000004; MBT8619011.1; -; Genomic_DNA.
DR EMBL; CP049634; QWD27948.1; -; Genomic_DNA.
DR EMBL; QMCH01000002; RAZ43062.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2Z4JPZ7; -.
DR KEGG; poh:DPM16_06220; -.
DR Proteomes; UP000248592; Chromosome.
DR Proteomes; UP000251072; Unassembled WGS sequence.
DR Proteomes; UP000676948; Chromosome.
DR Proteomes; UP000744877; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd06158; S2P-M50_like_1; 1.
DR InterPro; IPR008915; Peptidase_M50.
DR InterPro; IPR044537; S2P-M50-like.
DR PANTHER; PTHR35864; ZINC METALLOPROTEASE MJ0611-RELATED; 1.
DR PANTHER; PTHR35864:SF1; ZINC METALLOPROTEASE MJ0611-RELATED; 1.
DR Pfam; PF02163; Peptidase_M50; 2.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000313|EMBL:AWW50634.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000248592};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT TRANSMEM 57..76
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 96..120
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 179..198
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 14..134
FT /note="Peptidase M50"
FT /evidence="ECO:0000259|Pfam:PF02163"
FT DOMAIN 135..189
FT /note="Peptidase M50"
FT /evidence="ECO:0000259|Pfam:PF02163"
SQ SEQUENCE 220 AA; 24334 MW; 78492C2B1879C0C4 CRC64;
MITDYSIQAI AINAIPLIFA ITIHEAAHGY AARYFGDNTA YVLGRVSLNP AKHIDPIGTV
LVPLMLILAG SPFLVGYAKP VPVRFDRLHH PKTDSIWVAL AGPGANFIQA ILWAMAWIVI
QGLSIQEPFL TGMAQAGVMW NIGLLVFNLF PIPPLDGGRI LSGLLPTRQS LAFDRLERWG
FFIVLALVFT GIIGELWMQP LSNLFMRLID LLMTPLRMIF
//
