ID   A0A2Z4JTE3_9BURK        Unreviewed;      1202 AA.
AC   A0A2Z4JTE3;
DT   10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT   10-OCT-2018, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE            EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN   ORFNames=Pas1_06920 {ECO:0000313|EMBL:AWW50134.1};
OS   Polynucleobacter paneuropaeus.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Polynucleobacter.
OX   NCBI_TaxID=2527775 {ECO:0000313|EMBL:AWW50134.1, ECO:0000313|Proteomes:UP000248592};
RN   [1] {ECO:0000313|Proteomes:UP000248592}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MG-25-Pas1-D2 {ECO:0000313|Proteomes:UP000248592};
RA   Hahn M.W.;
RT   "Description of a new Polynucleobacter species.";
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00024632};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR   EMBL; CP030085; AWW50134.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2Z4JTE3; -.
DR   Proteomes; UP000248592; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd04485; DnaE_OBF; 1.
DR   CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR   Gene3D; 1.10.150.870; -; 1.
DR   Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR   Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR004805; DnaE2/DnaE/PolC.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR   NCBIfam; TIGR00594; polc; 1.
DR   PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; PHP domain-like; 1.
PE   4: Predicted;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW   DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Reference proteome {ECO:0000313|Proteomes:UP000248592};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          7..74
FT                   /note="Polymerase/histidinol phosphatase N-terminal"
FT                   /evidence="ECO:0000259|SMART:SM00481"
FT   COILED          490..520
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   1202 AA;  133384 MW;  EF794E5E50350B62 CRC64;
     MASPRFVHLR LHSEFSITDG IVRIDDAIAA AAKDEMGALA ITDLGNLFGL VRFYSGARAA
     GLKPIAGADV WISNPQDPDQ PHRLLLLVQN QSGYLNLCQL LSRASLDNQS RGRAEVNPQW
     FTEPAAKEED RKAKKTLAHG LIALSGARTG DVGAALLGGQ SDQAKVLAQR WSKAFPDSYF
     IEVQRGGHPQ DEQHLQFACH LASRLDLPVV ATHPVQFMQR SDFTAHEARV CIAEGELLGN
     PRRQKKFNEE QYFLSQVEME QRFADLPVAL ANSVEIAKRC NLSLTLGQPR LPNFPTPPGI
     TLDDYLLQQS EIGLKRHMER NFPDPAERTK EEGRYHDRLV FEVKTISQMG FPGYFLIVAD
     FINWAKNNGV PVGPGRGSGA GSLVAYSLGI TDLDPLRYNL LFERFLNPER VSMPDFDIDF
     CQQGRDRVIQ YVKDKYGKDA VSQIVTFGTM AARAAIRDVG RVLEQGYNFV DGIAKLVPNK
     PGQYMTIDMA KKEEKQLAER EKNEDEVRQL LSLAQQLEGM TRNVGMHAGG VLIAPGKLTD
     FCPLYTQETK DQDSSSVISQ FDKDDVEAIG LVKFDFLGLT TLTILAAAER WIKTLHADRK
     DWSIGDMALD DPAAFEVLKK ANTVAVFQLE SRGMQGMLRE AKPDRFEDII ALVALYRPGP
     MDLIPDFIER KHGRQKVEYP DPRIEPVLRE TYGIMVYQEQ VMQMAQMIGG YSLGGADLLR
     RAMGKKKPEE MAQHRKIFSD GAKAGGISEG KANEIYDLME RFAGYGFNKS HAAAYALLAY
     QTAWLKAHYP AEFMAANLSL AMDDTDKIKI LYDDCLANQI RVFSPDINTG VYDFTPLRSP
     DATPDAPLSH IRYGLGAVRG TGEAAIEAIV KAREAGGSFK DIFDFCMRVD RRQVNRRAIE
     ALIRAGAFDT LYKDQLQTGG NLFDIRSTLL ASLARAIEAA EQAEASVNQV SLFEVAGEAD
     RHLPELVREP VWSEKKRLSE EKTALGLCLT GHMFDAYREE TSHFIRQPLA KVTEGKDQLI
     AGIITSARML TGQRGRMMIA TIDDGSAAIE VTLYSEVYEP NRSWLKEDEL LVAKVNVTPD
     KFSGGMRVVA EAVMDITGAR MRFARNVHVC IDSAIDVKML RSQIGPYLMA NRTREPKFGA
     PPTASNDGVK GLILTAAVTT AGGACLIQFP EDLRVYPDDA CLHSLHQVLA SKQANPVQVQ
     YH
//