ID A0A2Z4LTV0_9FLAO Unreviewed; 485 AA.
AC A0A2Z4LTV0;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:AWX45335.1};
DE EC=3.2.1.1 {ECO:0000313|EMBL:AWX45335.1};
GN ORFNames=HME9304_02348 {ECO:0000313|EMBL:AWX45335.1};
OS Allomuricauda aurantiaca.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Allomuricauda.
OX NCBI_TaxID=1383885 {ECO:0000313|EMBL:AWX45335.1, ECO:0000313|Proteomes:UP000248536};
RN [1] {ECO:0000313|EMBL:AWX45335.1, ECO:0000313|Proteomes:UP000248536}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HME9304 {ECO:0000313|EMBL:AWX45335.1,
RC ECO:0000313|Proteomes:UP000248536};
RA Kang H., Kim H., Joh K.;
RT "Spongiibacterium sp. HME9304 Genome sequencing and assembly.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR EMBL; CP030104; AWX45335.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2Z4LTV0; -.
DR KEGG; spon:HME9304_02348; -.
DR OrthoDB; 9806009at2; -.
DR Proteomes; UP000248536; Chromosome.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 2.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Glycosidase {ECO:0000313|EMBL:AWX45335.1};
KW Hydrolase {ECO:0000313|EMBL:AWX45335.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000248536}.
FT DOMAIN 67..418
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 260
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 284
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 412
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 485 AA; 54993 MW; D83D2B493A065261 CRC64;
MKKSINSFFE SQANFFHKVI LISGCVLLAS CSDAFNQPSG LVEEALTNGD GQVSEGQDLT
NFLNGNGVMM QAFYWDVEPR FDWWDLIADK TDEWASVGID RIWLPPVSKG QSGGFSMGYD
PSDYYDLGEY FQQGTTETRF GSREELDNLI AKAHENDIEV IADIVMGHNS GGGLEYNPYR
DKDTFTLFNE MNGNASGMFN RNFEDYHPNS IHNNDEEALF FEEQDLCHLQ DNVQNWLWKF
DNSVAKYYKN TVGFDGWRFD YVKSFSPEYV DAWVDEVGGW SVGEFFDGNA NLVRDWVAAA
GVNAFDFPCL FQMRDAFQQN NLTILQNGDM LWKTNPADAV TFVANHDTDR EPVIEEEYKL
FAYSYILTHP GYPTIFYLDY ENEEFKEKLN QLILINRTIA SGELEVLFAD EDEYIAARKG
DGDNPGLVLY INTNGLELNR QVTTHWTENN LHDYTGSIAS SVATDSSGAV TLKAPANGYA
VWSIK
//
