ID   A0A2Z4V898_9ACTN        Unreviewed;       493 AA.
AC   A0A2Z4V898;
DT   10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT   10-OCT-2018, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   SubName: Full=rRNA cytosine-C5-methyltransferase {ECO:0000313|EMBL:AWZ16959.1};
GN   ORFNames=DRB96_37740 {ECO:0000313|EMBL:AWZ16959.1};
OS   Streptomyces sp. ICC1.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=2099583 {ECO:0000313|EMBL:AWZ16959.1, ECO:0000313|Proteomes:UP000250268};
RN   [1] {ECO:0000313|EMBL:AWZ16959.1, ECO:0000313|Proteomes:UP000250268}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ICC1 {ECO:0000313|EMBL:AWZ16959.1,
RC   ECO:0000313|Proteomes:UP000250268};
RA   Gosse J.;
RT   "Whole genome sequencing and metabolomic study of cave Streptomyces
RT   isolates ICC1 and ICC4.";
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RsmB/NOP family. {ECO:0000256|ARBA:ARBA00007494,
CC       ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR   EMBL; CP030287; AWZ16959.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A2Z4V898; -.
DR   Proteomes; UP000250268; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR   GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR   Gene3D; 1.10.940.10; NusB-like; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR   InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR   InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR   InterPro; IPR035926; NusB-like_sf.
DR   InterPro; IPR006027; NusB_RsmB_TIM44.
DR   InterPro; IPR023267; RCMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR22807:SF61; NOL1_NOP2_SUN FAMILY PROTEIN _ ANTITERMINATION NUSB DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR   Pfam; PF01189; Methyltr_RsmB-F; 1.
DR   Pfam; PF01029; NusB; 1.
DR   PRINTS; PR02008; RCMTFAMILY.
DR   SUPFAM; SSF48013; NusB-like; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS01153; NOL1_NOP2_SUN; 1.
DR   PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000250268};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW   ProRule:PRU01023};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01023};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}.
FT   DOMAIN          197..492
FT                   /note="SAM-dependent MTase RsmB/NOP-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51686"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        424
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         303..309
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         328
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         354
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         371
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ   SEQUENCE   493 AA;  52891 MW;  43455A69D9F0CAB5 CRC64;
     MSEQPRSRPA ATSGKGGGKP AKSGKPYRRP QKDPVRMLAF EVLRAVDERD AYANLVLPPL
     LKKARKDETF QARDAALATE LVYGTLRRQG TYDAVIKACI DRPLREVDPP VLDVLSLGAH
     QLLGTRIPTH AAVSASVELA RVVLGDGRAK FVNAVLRKIS AHDLEGWLEK VAPPYEDDAE
     EHLAVFHSHP RWVVSALWDA LGGGRAGIED LLEADNERPE VTLVARPGRS TTEELLEAVG
     EDSALPGRWS PYAVRMAEGG EPGALEAVRE GRAGVQDEGS QLVAMALAAA PVEGRDTRWL
     DGCAGPGGKA ALLAALAAER GAFLLASEKQ PHRARLVEKA LAGNPGPYQV ITADGTRPPW
     LPGSFDRVLV DVPCSGLGAL RRRPEARWRR RPEDLESFAP LQRGLLREAL AAVRVGGVVG
     YATCSPHLAE TRVVVEDVLK GRGAGAAPIA AELIDARPYM QGVPALGDGP DVQLWPHLHG
     TDAMYLALLR RTA
//