ID A0A314KR49_NICAT Unreviewed; 524 AA.
AC A0A314KR49;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU363044};
DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU363044};
GN ORFNames=A4A49_13449 {ECO:0000313|EMBL:OIT31796.1};
OS Nicotiana attenuata (Coyote tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=49451 {ECO:0000313|EMBL:OIT31796.1, ECO:0000313|Proteomes:UP000187609};
RN [1] {ECO:0000313|EMBL:OIT31796.1, ECO:0000313|Proteomes:UP000187609}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. UT {ECO:0000313|Proteomes:UP000187609};
RC TISSUE=Leaves {ECO:0000313|EMBL:OIT31796.1};
RA Xu S., Brockmoeller T., Gaquerel E., Navarro A., Kuhl H., Gase K., Ling Z.,
RA Zhou W., Kreitzer C., Stanke M., Tang H., Lyons E., Pandey P., Pandey S.P.,
RA Timmermann B., Baldwin I.T.;
RT "The genome of Nicotiana attenuata.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU363044};
CC -!- SIMILARITY: Belongs to the helicase family.
CC {ECO:0000256|RuleBase:RU363044}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OIT31796.1}.
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DR EMBL; MJEQ01001194; OIT31796.1; -; Genomic_DNA.
DR RefSeq; XP_019226835.1; XM_019371290.1.
DR RefSeq; XP_019226836.1; XM_019371291.1.
DR RefSeq; XP_019226837.1; XM_019371292.1.
DR AlphaFoldDB; A0A314KR49; -.
DR STRING; 49451.A0A314KR49; -.
DR EnsemblPlants; OIT31796; OIT31796; A4A49_13449.
DR GeneID; 109208225; -.
DR Gramene; OIT31796; OIT31796; A4A49_13449.
DR KEGG; nau:109208225; -.
DR OrthoDB; 1220275at2759; -.
DR Proteomes; UP000187609; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd18809; SF1_C_RecD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR010285; DNA_helicase_pif1-like.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049163; Pif1-like_2B_dom.
DR PANTHER; PTHR10492:SF57; ATP-DEPENDENT DNA HELICASE; 1.
DR PANTHER; PTHR10492; UNCHARACTERIZED; 1.
DR Pfam; PF05970; PIF1; 1.
DR Pfam; PF21530; Pif1_2B_dom; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|RuleBase:RU363044};
KW DNA damage {ECO:0000256|RuleBase:RU363044};
KW DNA recombination {ECO:0000256|RuleBase:RU363044};
KW DNA repair {ECO:0000256|RuleBase:RU363044};
KW Helicase {ECO:0000256|RuleBase:RU363044};
KW Hydrolase {ECO:0000256|RuleBase:RU363044};
KW Nucleotide-binding {ECO:0000256|RuleBase:RU363044};
KW Reference proteome {ECO:0000313|Proteomes:UP000187609}.
FT DOMAIN 358..404
FT /note="DNA helicase Pif1-like 2B"
FT /evidence="ECO:0000259|Pfam:PF21530"
SQ SEQUENCE 524 AA; 58850 MW; 952140C653050006 CRC64;
MPTPVFNEEE FDNTNRLICE ELRYNRCSLS KEHEELFAKL TIEQKLVYDR IIKSVHEEKG
EFFFLYGHGG TGKTFIWRTL SSAIRCKGDI VLTVASSGIA SLLLPGGRTA HSRFAIPLNA
NEDSTCNITQ GTPLSKLIVK TKLIIWDEAP MMHRYCFEAL DRTLRDILRF KDASNLDRPF
GGKTVVLGGD FRQILPVIPK GTRQDIVNAT LNSSYLWNHC QVLKLTKNMR LEGNLEDSHL
NDLRQFSDWI LAIGNGMIGN SVDGIDKVQI PYDLIINNCG DPISAIVEST YPDFLSHCSD
ITYLQQRGIL APTLDMVESI NEYMVSVNKS QPKIFFSSDT ICMSDDAFTG LEHIHTPEFL
NTIKCSGIPN HVITLKVGVP VMLLRNIDPS SGLCNGTRLI ITRLGNRVIE AKILSGNMAG
QKVFIPRMSL TPSDAKIPFK FQRRQFPIVV SFAMTINKSQ GQSLSHVGLY LKKPVFTHGQ
LYVALSRVTK RKGLKILVYD DDGEISNEVI NVVYKEVFRN LLGQ
//
