ID   A0A314LE32_NICAT        Unreviewed;       622 AA.
AC   A0A314LE32;
DT   10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT   10-OCT-2018, sequence version 1.
DT   22-FEB-2023, entry version 16.
DE   RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE            EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN   ORFNames=A4A49_18847 {ECO:0000313|EMBL:OIT39812.1};
OS   Nicotiana attenuata (Coyote tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=49451 {ECO:0000313|EMBL:OIT39812.1, ECO:0000313|Proteomes:UP000187609};
RN   [1] {ECO:0000313|EMBL:OIT39812.1, ECO:0000313|Proteomes:UP000187609}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. UT {ECO:0000313|Proteomes:UP000187609};
RC   TISSUE=Leaves {ECO:0000313|EMBL:OIT39812.1};
RA   Xu S., Brockmoeller T., Gaquerel E., Navarro A., Kuhl H., Gase K., Ling Z.,
RA   Zhou W., Kreitzer C., Stanke M., Tang H., Lyons E., Pandey P., Pandey S.P.,
RA   Timmermann B., Baldwin I.T.;
RT   "The genome of Nicotiana attenuata.";
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00000966,
CC         ECO:0000256|RuleBase:RU361166};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC       ECO:0000256|RuleBase:RU361166}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OIT39812.1}.
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DR   EMBL; MJEQ01000078; OIT39812.1; -; Genomic_DNA.
DR   RefSeq; XP_019259484.1; XM_019403939.1.
DR   AlphaFoldDB; A0A314LE32; -.
DR   STRING; 49451.A0A314LE32; -.
DR   EnsemblPlants; OIT39812; OIT39812; A4A49_18847.
DR   GeneID; 109237619; -.
DR   Gramene; OIT39812; OIT39812; A4A49_18847.
DR   KEGG; nau:109237619; -.
DR   OrthoDB; 1347382at2759; -.
DR   Proteomes; UP000187609; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR019028; CBM_49.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF19; ENDOGLUCANASE 19-RELATED; 1.
DR   Pfam; PF09478; CBM49; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SMART; SM01063; CBM49; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW   ECO:0000256|PROSITE-ProRule:PRU10059};
KW   Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW   ECO:0000256|RuleBase:RU361166};
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW   ProRule:PRU10059};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU10059};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW   ECO:0000256|PROSITE-ProRule:PRU10059};
KW   Reference proteome {ECO:0000313|Proteomes:UP000187609};
KW   Secreted {ECO:0000256|ARBA:ARBA00022525};
KW   Signal {ECO:0000256|RuleBase:RU361166}.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000256|RuleBase:RU361166"
FT   CHAIN           27..622
FT                   /note="Endoglucanase"
FT                   /evidence="ECO:0000256|RuleBase:RU361166"
FT                   /id="PRO_5016189450"
FT   DOMAIN          530..610
FT                   /note="Carbohydrate binding"
FT                   /evidence="ECO:0000259|SMART:SM01063"
FT   ACT_SITE        416
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        468
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        477
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ   SEQUENCE   622 AA;  68643 MW;  814983B1E34634CB CRC64;
     MNFVMMFCAM TPQLLIIWLL PNVAFASSHN YGEALSKSFL FYEAQRSGYL PHDQRVQWRG
     NSGLLDGKAS GVDLVGGYYD AGDNVKFGLP MAFTVTMMSW SIIEYGKQMG ESGELSNAMD
     AVKWGTDYLL KAHPEPNVLY GEVGDGTTDH YCWQRPEDMT TSRAAYRIDP SRPGSDLAGE
     TAAAMAAASI VFRNSNPAYA KELLTHAFQL FEFADKYRGK YDSSITVAQK YYRSVSGYAD
     ELLWAAAWLY KASNKPYYLN YLGENGDALG GTGWSMTEFG WDVKYAGVQT LAAKFLMQGN
     AGKHEPVFDK YQEKAENFMC ACLGKGNQNI HKSPGGLIFR QRWNNMQFVT SASFLATVYS
     DYLASARKSL KCSSGTVSPS ELLSFAKSQV DYILGDNPRA TSYMVGYGNN YPRQVHHRGS
     SIVSVKKDPS FVSCRGGYAT WFSRKASDPN LLAGAIVGGP DAYDNFADQR DNYEQTEPAT
     YNNAPLIGVL ARLHGGQSQY SQLLPVAIPQ PKPDPEQKVT RAPASSTADI TIEQKETASW
     VHKGKTYYRY SVIVTNNSAK TLKNLKLSIS QLYGSLWGLS KYGDSYVFPA WLNSLPAGKS
     LEFVYVHSAS PAVVSISSYT LV
//