ID A0A317L3A9_9BACI Unreviewed; 369 AA.
AC A0A317L3A9;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 24-JAN-2024, entry version 13.
DE RecName: Full=Pectate lyase {ECO:0000256|ARBA:ARBA00012272, ECO:0000256|RuleBase:RU367009};
DE EC=4.2.2.2 {ECO:0000256|ARBA:ARBA00012272, ECO:0000256|RuleBase:RU367009};
GN ORFNames=DLJ74_00235 {ECO:0000313|EMBL:PWU70307.1};
OS Gracilibacillus dipsosauri.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Gracilibacillus.
OX NCBI_TaxID=178340 {ECO:0000313|EMBL:PWU70307.1, ECO:0000313|Proteomes:UP000245624};
RN [1] {ECO:0000313|EMBL:PWU70307.1, ECO:0000313|Proteomes:UP000245624}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DD1 {ECO:0000313|EMBL:PWU70307.1,
RC ECO:0000313|Proteomes:UP000245624};
RA Deutch C.E., Yang S.;
RT "Genomic analysis of Gracilibacillus dipsosauri DD1 reveals novel features
RT of a salt-tolerant amylase.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the depolymerization of both polygalacturonate and
CC pectins of methyl esterification degree from 22 to 89%, with an endo
CC mode of action. In contrast to the majority of pectate lyases, displays
CC high activity on highly methylated pectins.
CC {ECO:0000256|RuleBase:RU367009}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC their non-reducing ends.; EC=4.2.2.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000695,
CC ECO:0000256|RuleBase:RU367009};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913,
CC ECO:0000256|RuleBase:RU367009};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|RuleBase:RU367009}.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 3 family.
CC {ECO:0000256|ARBA:ARBA00006463, ECO:0000256|RuleBase:RU367009}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWU70307.1}.
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DR EMBL; QGTD01000001; PWU70307.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A317L3A9; -.
DR OrthoDB; 148600at2; -.
DR Proteomes; UP000245624; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030570; F:pectate lyase activity; IEA:UniProtKB-UniRule.
DR CDD; cd00161; RICIN; 1.
DR Gene3D; 2.80.10.50; -; 3.
DR Gene3D; 2.160.20.10; Single-stranded right-handed beta-helix, Pectin lyase-like; 1.
DR InterPro; IPR004898; Pectate_lyase_PlyH/PlyE-like.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR PANTHER; PTHR33407; PECTATE LYASE F-RELATED; 1.
DR PANTHER; PTHR33407:SF9; PECTATE LYASE F-RELATED; 1.
DR Pfam; PF03211; Pectate_lyase; 1.
DR Pfam; PF14200; RicinB_lectin_2; 2.
DR SMART; SM00458; RICIN; 1.
DR SUPFAM; SSF51126; Pectin lyase-like; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|RuleBase:RU367009};
KW Lyase {ECO:0000256|RuleBase:RU367009, ECO:0000313|EMBL:PWU70307.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000245624};
KW Secreted {ECO:0000256|RuleBase:RU367009};
KW Signal {ECO:0000256|RuleBase:RU367009}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|RuleBase:RU367009"
FT CHAIN 27..369
FT /note="Pectate lyase"
FT /evidence="ECO:0000256|RuleBase:RU367009"
FT /id="PRO_5025086368"
FT DOMAIN 32..169
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
SQ SEQUENCE 369 AA; 40798 MW; 4AB45478912870FE CRC64;
MRAKLLTMLA FALLLVGAIS VESLEAASFD SSKSYKIINR FSGKALEVYE WSTSDGGNIV
QYDDLGGLNQ QWRIINVGEG YHKIVNQNSI KAMEVYDWST EDGANVSQWY DWNAANQQWA
IEGIGNGYVK IVNRHSGKAL EVFDWSTENG ANVVQWDDWG GESQQWRIIE VPPSDTITVN
QTIVVESGEV FDGEGKRYVA NPDTVGDGSQ GEDQDPVFRL EDGATLKNVV IGHPAADGVH
TYGDVLVQNV VWEDIGEDAL TIKDDGHVTI RGGMAQKGSD KMFQVNAPST FEIINFTADD
AGKMIRQNGG TTFKTSIFID GSVITNMDEA IFRTDSDSSE VTMTNTRYSD VGRKWYNVEN
VSESGNYEY
//
