ID   A0A317MQS0_9GAMM        Unreviewed;       362 AA.
AC   A0A317MQS0;
DT   10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT   10-OCT-2018, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   SubName: Full=Pyruvate formate lyase activating enzyme {ECO:0000313|EMBL:PWV59035.1};
GN   ORFNames=C7443_11233 {ECO:0000313|EMBL:PWV59035.1};
OS   Plasticicumulans acidivorans.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria;
OC   Candidatus Competibacteraceae; Plasticicumulans.
OX   NCBI_TaxID=886464 {ECO:0000313|EMBL:PWV59035.1, ECO:0000313|Proteomes:UP000246569};
RN   [1] {ECO:0000313|EMBL:PWV59035.1, ECO:0000313|Proteomes:UP000246569}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 23606 {ECO:0000313|EMBL:PWV59035.1,
RC   ECO:0000313|Proteomes:UP000246569};
RA   Goeker M.;
RT   "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT   most valuable type-strain genomes for metagenomic binning, comparative
RT   biology and taxonomic classification.";
RL   Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|PIRSR:PIRSR004869-50};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000256|PIRSR:PIRSR004869-50};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PWV59035.1}.
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DR   EMBL; QGTJ01000012; PWV59035.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A317MQS0; -.
DR   OrthoDB; 9778883at2; -.
DR   Proteomes; UP000246569; Unassembled WGS sequence.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR027596; AmmeMemoSam_rS.
DR   InterPro; IPR034457; Organic_radical-activating.
DR   InterPro; IPR016431; Pyrv-formate_lyase-activ_prd.
DR   InterPro; IPR007197; rSAM.
DR   NCBIfam; TIGR04337; AmmeMemoSam_rS; 1.
DR   PANTHER; PTHR30352; PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME; 1.
DR   PANTHER; PTHR30352:SF5; TRNA 4-DEMETHYLWYOSINE SYNTHASE (ADOMET-DEPENDENT); 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   PIRSF; PIRSF004869; PflX_prd; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01101; Uncharacterised_Radical_SAM_Su; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR004869-50};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRSR:PIRSR004869-
KW   50}; Lyase {ECO:0000313|EMBL:PWV59035.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR004869-50}; Pyruvate {ECO:0000313|EMBL:PWV59035.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000246569};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PIRSR:PIRSR004869-50}.
FT   DOMAIN          75..292
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
FT   BINDING         90
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004869-50"
FT   BINDING         94
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004869-50"
FT   BINDING         97
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004869-50"
SQ   SEQUENCE   362 AA;  40009 MW;  051269C1BB93873E CRC64;
     MSAQAFTLSH PARYWHALAD GRLQCDLCPR YCHLHEGSRG LCFVRMREGD AIVLDTYGRS
     SGFCVDPIEK KPLNHFLPGT PVLSFGTAGC NLACKFCQNW DISRSRDTDR LAAHAGPEDI
     AATAQRLGCR SVAYTYNDPV IFLEYAVDVA AACHARGLRN VAVTAGYICA EPRVEFFAAM
     DAANVDLKAF TEDFYHRVCG GHLQAVLETL EYLHHETSVW LEITTLLIPG YNDSDAEVEQ
     LSGWIAGHLG ADVPLHFTAF HPDWKFDQVP ATPAATLRRA RGIARRQGLH HVYTGNVHDV
     EGGSTYCAGC GECLIERDVY RLGRWRLDEQ GCCLNCGRRL PGVFEAHPGD WGSRRLPVAC
     GQ
//