ID A0A317P9H4_9GAMM Unreviewed; 801 AA.
AC A0A317P9H4;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN ORFNames=DER72_1016 {ECO:0000313|EMBL:PWV82828.1};
OS Halomonas sp. A11-A.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Halomonas.
OX NCBI_TaxID=2183985 {ECO:0000313|EMBL:PWV82828.1, ECO:0000313|Proteomes:UP000246506};
RN [1] {ECO:0000313|EMBL:PWV82828.1, ECO:0000313|Proteomes:UP000246506}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A11-A {ECO:0000313|EMBL:PWV82828.1,
RC ECO:0000313|Proteomes:UP000246506};
RA Wrighton K.;
RT "Subsurface microbial communities from deep shales in Ohio and West
RT Virginia, USA.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU000442};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755, ECO:0000256|RuleBase:RU000442}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWV82828.1}.
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DR EMBL; QGTM01000001; PWV82828.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A317P9H4; -.
DR Proteomes; UP000246506; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd05784; DNA_polB_II_exo; 1.
DR CDD; cd05537; POLBc_Pol_II; 1.
DR Gene3D; 2.40.50.590; -; 2.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 2.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10322; DNA POLYMERASE CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR10322:SF23; DNA POLYMERASE DELTA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 2.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF21474; DNApolII_N; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|RuleBase:RU000442};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU000442};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU000442};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU000442};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000442}.
FT DOMAIN 88..308
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 390..444
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT DOMAIN 502..779
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
SQ SEQUENCE 801 AA; 90143 MW; 2C60C115F09523F0 CRC64;
MTHHGSTRGE GITYQGFILT RHWEETPRGT EVTLWLATDQ GPLKVRLPVQ PSVAFLPVEQ
RAAAEALLAG EAGVTLRELE LRDFHHRPVL GLYCEQYRQL LRLERRLGEA GLALYEADIR
PPERYLMERF ITAPVEVTGK VDGQGGLVEA RLRPAPAYRP RLRTVSLDIE TSARGELYSI
ALEGCGQRQV YMLGPANGGE GERDFALEYC ASRAELLRRL NAWFAEHDPD AIIGWNLVQF
DLRVLRDHAE RLGVPLRLGR GGSELEWRAH GHQPNHFFAA TAGRLIIDGI EALRSATWHF
PSFSLESVAR ELLGEGKAID NPYQRLDEIL RLFAEDKPAL ARYNLQDCEL VTRIFARAEL
LDFLLERAAV TGLPADRSGG SVAAFTHLYL PRLHRLGLVA PSLGEGEDAE SPGGFVMDSR
PGLYDSVLVL DFKSLYPSII RSFLIDPAGL VEGLADPSDA ASVPGFRGAR FSRTRHALPA
IVEGVWVGRE AAKRAGNAPL SQALKIIMNS FYGVLGSKGC RFFDARLASS ITMRGHAIMH
RTRELIEGEG YTVIYGDTDS TFVWLGRPCE EAEAAAIGHR LVEQVNAWWR EHLAEAYGLA
SALELQFETH YRRFLMPTIR GAEAGSKKRY AGVVRDAEGQ KRLVFKGLET VRTDWTELAK
AFQQELYRRV FTGEPWREYV RDYVQAFLAR ARSGEFDERL VYRKRLRRRL DDYRRNVPPH
VRAARLADAE NDRLGRPRQY QHGGWIRYVM TLAGPEPLEA HRSPIDTDHY LARQLQPVAD
AILPFVGDDF ASLIDRQLGL F
//
