ID   A0A318E3Q3_9GAMM        Unreviewed;       531 AA.
AC   A0A318E3Q3;
DT   10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT   10-OCT-2018, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   SubName: Full=Hydrogenase large subunit {ECO:0000313|EMBL:PXV65674.1};
GN   ORFNames=C8D93_10953 {ECO:0000313|EMBL:PXV65674.1};
OS   Sinimarinibacterium flocculans.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales; Nevskiaceae;
OC   Sinimarinibacterium.
OX   NCBI_TaxID=985250 {ECO:0000313|EMBL:PXV65674.1, ECO:0000313|Proteomes:UP000248330};
RN   [1] {ECO:0000313|EMBL:PXV65674.1, ECO:0000313|Proteomes:UP000248330}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 104150 {ECO:0000313|EMBL:PXV65674.1,
RC   ECO:0000313|Proteomes:UP000248330};
RA   Goeker M.;
RT   "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT   most valuable type-strain genomes for metagenomic binning, comparative
RT   biology and taxonomic classification.";
RL   Submitted (APR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC         Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC         Evidence={ECO:0000256|ARBA:ARBA00001967,
CC         ECO:0000256|PIRSR:PIRSR601501-1};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC       Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC       family. {ECO:0000256|ARBA:ARBA00009292}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PXV65674.1}.
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DR   EMBL; QICN01000009; PXV65674.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A318E3Q3; -.
DR   OrthoDB; 9761717at2; -.
DR   Proteomes; UP000248330; Unassembled WGS sequence.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   PANTHER; PTHR42958:SF4; HYDROGENASE EXPRESSION_FORMATION PROTEIN HUPK; 1.
DR   PANTHER; PTHR42958; HYDROGENASE-2 LARGE CHAIN; 1.
DR   Pfam; PF00374; NiFeSe_Hases; 2.
DR   SUPFAM; SSF56762; HydB/Nqo4-like; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
PE   3: Inferred from homology;
KW   Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW   Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR601501-1};
KW   Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|PIRSR:PIRSR601501-1};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000248330}.
FT   BINDING         43
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT   BINDING         62
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT   BINDING         65
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT   BINDING         65
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT   BINDING         461
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT   BINDING         509
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT   BINDING         512
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT   BINDING         515
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ   SEQUENCE   531 AA;  60015 MW;  7686D96148EF54A6 CRC64;
     MAVQTLDISP VGRVEGDLDV RVDIDNGVVT NAWTQAELFR GFEIILRDKD PQAGLVVTPR
     ACGICGASHL TCAAWALDTA WGTEVPRNAI LARNLGQLTE SIQSQPRYFY GLYAIDMTNK
     KYRNSPFYEE AVKRFAPFTG TSYEQGVTIS GKPVEIYALL GGQWPHSSYM VPGGVMCAPT
     LTDITRAWSI LEYYKKNWLE SLWLGCTLER YEQIKTHDDF LAWLDERPEH ANSDLGFYWR
     MGLDIGLDKY GGGIGKYVSW GYLPHEDRYQ KPTIDGRNAA VIMKSGVYDG ESDTHMLMDQ
     KFLRENTTHA WYDEGGQDIH PFDRTTKPIQ KNDLDHAGKY SWATSVRHIE SGRLEAGPFA
     RQLIAGGRHG ESWQHYDGLI LDMYKKMGGP SLHLRQIARM HETIKLYREC ERILREFRLN
     DPWYIKPKEK DGRGWGATEA IRGALCHWIE VKDGKIKNYQ IIAPTTWNVG PRDGGEKLGP
     IEQALVGTPI ADPTDPVEVG HVARSFDSCL VCTVHAHDKK TGKELARFRT A
//