ID A0A318KEW1_9NOCA Unreviewed; 5139 AA.
AC A0A318KEW1;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=Pimaricinolide synthase PimS1 {ECO:0000313|EMBL:PXX71475.1};
DE Flags: Fragment;
GN ORFNames=DFR70_101901 {ECO:0000313|EMBL:PXX71475.1};
OS Nocardia tenerifensis.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae;
OC Nocardia.
OX NCBI_TaxID=228006 {ECO:0000313|EMBL:PXX71475.1, ECO:0000313|Proteomes:UP000247569};
RN [1] {ECO:0000313|EMBL:PXX71475.1, ECO:0000313|Proteomes:UP000247569}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44704 {ECO:0000313|EMBL:PXX71475.1,
RC ECO:0000313|Proteomes:UP000247569};
RA Goeker M.;
RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT most valuable type-strain genomes for metagenomic binning, comparative
RT biology and taxonomic classification.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PXX71475.1}.
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DR EMBL; QJKF01000001; PXX71475.1; -; Genomic_DNA.
DR Proteomes; UP000247569; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd08956; KR_3_FAS_SDR_x; 3.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 3.30.70.3290; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 3.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 3.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR020802; PKS_thioesterase.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR001031; Thioesterase.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 3.
DR Pfam; PF21089; PKS_DH_N; 3.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF14765; PS-DH; 3.
DR Pfam; PF00975; Thioesterase; 1.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00826; PKS_DH; 3.
DR SMART; SM00822; PKS_KR; 3.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SMART; SM00824; PKS_TE; 1.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 6.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 3.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..176
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1368..1443
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1461..1878
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3047..3122
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3140..3552
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4766..4841
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 4446..4481
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4450..4469
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:PXX71475.1"
SQ SEQUENCE 5139 AA; 537944 MW; E84AB362B947BB47 CRC64;
ARRNGHPVLA VVAGSAVNSD GASNGLTAPS GPSQQRVIRQ ALANAGVSPA EVDVVEAHGT
GTRLGDPIEA QALLATYGQG RPQDRPLWLG SIKSNMGHTQ AAAGAAGIIK MVMAMRHATL
PKTLHAETPT PQVDWGSGAV ELLTETRPWP PAEHPRRAAV SSFGISGTNA HVIIEQAPPT
ETPEPLPDSM RAAPMLLSGK SAGALRDQAR ALLAHLDDDP EPTVADFAVS LMNTRATFEH
RAVVAAADRD GFRSALRALA EDSHHPYLTQ GRSDVTGKTV FVFPGQGSQW HGMAVELLAS
APVFAEHIRL CAEALEPFLD WSLLDVLTGT ASAPPLERVD VVQPVLFSMM VSLAALWRSC
GVEPRAVVGH SQGEIAAAVV AGGLSLHDGA RIVALRSKAL RALTGKGAMM FVAQEAGLLR
ERLNARGGSL SVAAVNGPAS VVVSGDPDEL DALGVELSEE GVFRWMVPGV DFAAHSVHVE
EIRDELAAVL AAVEPRAGTV PFFSTMLGDW VGTVDLDAEY WYRNLREPVE FERAARGLAE
AGYDTFVEVS PHPVITTWVQ QTVDAIRPDN VIAGSLHRED GGLDRFLSSL GALHVRGVRV
DWSAVSPVGR RVDLPTYPFQ RERFWAAEPS SGGDPRSLGL RAAAHPLLGA VVALADSDGV
VFTGSLSTST QPWLAEHDVF GRVLLPGTGF VELAIRAGDE VGCGTVEELT LRAPLLLPDG
DPVVVQIVVG APDETGRRPL SIHSRADDPA APWTVHADGV LGNCTSTADF DLAQWPPEGA
EPVPVADAYA VLSGEGYGYG PVFQGLHAAW RRGDEVFAEV ALPEQARPDA ERFGLHPALL
DAAMHIAILE DGGHRDGETV LPFAWNGVAL HAVGAAALRV RIAPAASDAI AVQVADSAGR
PVLSVASLVS RPVSADQMSA AGQDDSVFRI EWQPAPSPTV RPSIGAWDAL ADEEPVPDVV
VFECPEADGE TPGAARTVAY RTLEVVQNWL ADPRFAEARL LVVTRNAVAT QPEAAVALNQ
APVWGLVRAA QAENPDRFVL ADIDEPAAVS IAAAVSDESE LAVRAGRVLV PRLVRVASTA
DRAEPEWNST GTVLITGGTG GIGALIARHL VVRHGVRRLV LTSRSGPAAA GAEELRAELR
TLGAEVTIAA CDVADRAALA ALIAAVPADH PLTGVVHAAG VAYNGLVGAL TPDLIDGVLA
AKADGAWHLH ELTRDLDLSA FVLFSSAGGM ILAAGQGGYA AANVFLDALA VHRRAEGLPA
TTLAWGLWGI EAGLSQWLTD ADRKRLRRQG IPAFTADEGL ALFDAALSLD EPLLVPMRVD
TTALRTRTDT IPALLRGFVR TSIRQVAGAD GTGLAQRLAG LTEERRAEAV LNLVRTHVAA
VLGHASPDAV APDRAFQELG FDSLGAVELR NQLNAATALR LPATLIFDYP TSAAVAEHLV
ERLAGSVTVA GAVAARTSSD DEPIVIVGMS CRYPNGVSSP EDLWRLVADG VDAVTEFPGD
RGWHLDDVYD PEPGVPGKTY AREGGFLYGA GGFDAAFFGI SPNEALYMDP QQRLLLECSW
EALERAGIEA NSLRGSATGV FAGLMYHDYG LGVEAASASG GSLVSGRLSY VFGLEGPSVT
VDTACSSSLV ALHLAGQALR SGECSLALAG GVAVMGTPGM FVEFSRQRGL SADGRCKSFS
ANADGTGWAE GVGVLVLERL SDARRNGHPV LAVVAGSAVN SDGASNGLTA PSGPSQQRVI
RQALANAGVA PADVDAVEAH GTGTTLGDPI EAQALLATYG QDRPADRPLW LGSIKSNIGH
AQAAAGVGGI IKMVMAMRHG VLPKTLHVTE PTRQVDWTDG AVELLTEARA WPEVDRPRRA
GVSSFGLSGT NAHVIIEQAP EAVPEPERVA LPVIPWVLSG KTKRSLGEQA ERLRAYVADR
PELDPADVAY TLATERVGFE HRAVVIGTSS ESLISELAGV AGGAGVTDSV RVGKTAAVFS
GQGAQRLRMG RALYAAYPVF ATAFDAVADE LDAQLERPLR EVMWGDDAEQ LAQTMWAQAG
LFAFEVALFR LFESWGVRAD FVAGHSIGEL TAAHVAGALS LPDAARLVAA RGRLMQALPD
GGVMIAVQAS EEEIVADLVE GVSIAAVNAP GSVVLSGIAE QVRAVAERWE SRGRKTTRLR
VSHAFHSALM EPMLAEFRAV AESIAYATPE LAVVSNLTGQ PVERFDADHW VRHLRATVRF
ADGVRYLATA GVTRYLELGP DAVLAAMIQH TLDDTEITAA ARVVPTLRAE HEETIAVVTA
LARLHAAGAR VDWARFFAGS GARRVDLPTY PFEHHNYWLA PKTVSSDATA IGLEAIEHPL
LGAIVPAPET DTVVLTGRLS VSSQPWLADH SVLGTVLLPG TAFVDLAIRA GDQVGCNTLE
ELTIQAPLVL PARGGVQFQV SVGPLADTGD RTVTIHARDE HHLTAPWTLC AEGVLSERIT
EPASTAADWP PPGATPLPVD DLYPVLADRG FAYGPTFHCV TAAWTSGDEF YAEVALPEQA
RADGERFGVH PALLDAAAHV VLGAGDGEAA LPFAWQGIAL HAAGATEVRV RIKPVGANSI
SLDLTDLTGA PVLSVRSLAA RPVSTAQLGS SHTAAPNSLF ALTWTQVAVA DAAPIHVAEW
GSAFDDAPDV VVLTTERGND AEAVRAQTWR VLDRARTWLA DERFAAATLA IVTDGAVALP
GEDVTDLAGA AVWGLVRAAQ AEHPGRIVLV DTDGSLDVSV ALASAEPQLV LRNGAVHVPR
LAAVPLPDAT GHERFGTGPV LVSGASGMLG RLVSRHLAEQ GVRGLVLVSR RGAAADGMAE
LCADLAAVGV EVEVVACDLA DRAAATELLD GRALTGVVHV AGVLDDALLG ALTPERVDAV
LRPKVDAAMN LHELTRDLSA FVLFSSAAGI LGNAGQANYA AANAFLDALA AHRRANGLPA
QSLAWGMWAD RAGMSGRLTE AGLQRLARSG MGALSHEQGL ALFDAAAAVD APVLLPIHLD
IAALRTAADE LPSLFAGLVR GRSRRRAATA AAATDRLAAL PATERLDAVL ELVRGRAAAV
LGHHGADAVA ADRAFTELGF DSLSAVEFRN ALAEAVGLRL PATLVFDYPN AQSVAEYVAG
LLSGAAASTR AVAATSGNPD EPIAIVGMAC RYPGGVDSPE GLWRLVAEGV DAVGEFPADR
GWDVGRIYDP EPGVAGKSYV REGGFLYQAG GFDPAFFGIS PNEAFGMDPQ QRLLLECSWE
AFERAGIDPN ALRGSDTGVF AGMMYHDYAA NSATGAIASG RLSYVYGLEG PSVTVDTACS
SSLVALHLAS QALRSGECSL ALAGGVAVMA TPDPFIEFSR QRGLSADGRC RSFAADADGT
GWSEGVGVLV VERLSDARRL GHPVLALVRG VAVNQDGASN GLTAPNGPSQ QRVIRQALAN
AGLSFADVDA VEAHGTGTKL GDPIEAQALL ATYGQERSQD RPLWLGSIKS NIGHAQAAAG
VGGIIKMIMA MRHGVLPKTL HVNEPTPQVD WSEGAVELLT EARPWPRLDR PRRAGISSFG
ISGTNAHVVI EQGPDTDPAE TPGSATVVPW LLSAKTSRAL TDQAQRLRSF LADHPDAKPV
DIAFTLATDR VAFQHRAAVI GSSRADFATA LADLDVVPLS AAAGKTAMVF SGQGAQRLRM
GQRLHAEYPV FAEAFDAVAA ELEPWLDRPL REVVWGDDPD LLDQTRWTQP GLFAVEVALF
RLLESWGVRH DFVAGHSIGE LTAAYVAGVM SLPDAARLVA ARGRLMQSLP DGGVMIAVQA
SEAEIVPDLV EGVSIAAINA PGSVVLSGAA ERVIAVASGW EAKGRKTTRL RVSHAFHSAL
MEPMLAEFAA VAETIAYRAP ELPVVSNLTG RLVERFDADY WVRHIRETVR FADGVRCLAD
AGVSRFLEIG PDAVLATMVR QTLDEIASTG GGFGDTIDHG PSVLPTLRAH RDETSAVVTA
LARLHGSGAP VDWARFFAGS GARRVDLPTY PFQHETYWLT GDSPQDDPAS IGLDAVDHPL
LGARVVLPDG ATMLTGRLSV DTQPWLAEHR VGGSILFPGT GFVELAVRAG DAVGCAVLQE
LVLHAPLVLP EQGGVQLQVM VGEPDESATR TVTIHSRHGE QPWTLHARGV LAEHAPRPSF
DLMHWPPANA TPVDLADAYE TLADNGYHYG PVFRGLRAAW TAGDDLYAEV ALPDTAGDEA
ARYGIHPALL DACLHALRFG GAADDAELVL PFSWTDVALH AGGASVLRVR ITDLGADSAA
FAIADGTGRP VLSVGALMSR PVPAAQLNQA TSAFHDDLFE VAWSPVPLAP AEVSMADWST
VAAGGPVPEL VVLTVDPGND AAAVHATTHH VLAVLQNWLA DARYDQSALA VVTHGAVARE
NEDVTDLAGA AVWGLVRSAQ SEDPGRIMLA DTDDSLELRA ALARGETQVM VRDGAVFTAR
LGRIPVAEPG EGEKPGEDGR LDESGKLDHN GRPGAIGKLG EGERHSGGID FGAGQVLVSG
ASGRLGKLLA RHLVDRGARQ LMLVSRRGPA ADGMAELSAE LTDLGAEVEL IACDLADRAA
AARLLDGRAL SAVVHVAGVL DDAVIGSLTP ERMDTVLRPK VDAALHLHEL TADLSAFVLF
SSISGVFGNP GQGNYAAANA FLDALATHRR AHGLPAQSLA WGMWGGATGM AGGLDDADRK
RLARNGIEAL TEERGLALFD TATALDAPVL AAVRLDRTAL AAAGSDLPPL FDGLVTTVRR
RTAAGTVDAG SLRRALAGLD EDAQVAHLVT VIQRGAAALL GHADPADIDP ARDFLETGFD
SLAAMELRNT LGKALGLRLP PMVVFDNKSP AELARFLRAE LDADPAATQP PTNRSGGADT
ISALFRAAVY ADRLPQGFEL LTATAALRPS FASAADLDTG TQPLTLAEGP ADPRLICIST
PMATGGVFQN ARLAAGFRDL RSVLAVPLSG FAAGEPLPAT AAAAIEVIAD SVLAAADGKP
FAVLGYSAGG TLAYAVAEHL EERGVVPAGV VLLDTYRSEG GGDIGIAKEL VFGMLDMESA
FGGFDSARLS SMGRYADLLR GIAPGRIAAP VLFVQCLESF FGEPADAADI GWQAQPWDDT
QTLRTVAANH FTMLESQAGA TAEVIEHWLD ALPRTAPTP
//
