ID   A0A327LNU0_9RHOB        Unreviewed;       969 AA.
AC   A0A327LNU0;
DT   10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT   10-OCT-2018, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE            EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE   AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN   ORFNames=DOO74_19445 {ECO:0000313|EMBL:RAI52086.1};
OS   Rhodobacteraceae bacterium AsT-22.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC   Paracoccaceae.
OX   NCBI_TaxID=2230886 {ECO:0000313|EMBL:RAI52086.1, ECO:0000313|Proteomes:UP000249713};
RN   [1] {ECO:0000313|EMBL:RAI52086.1, ECO:0000313|Proteomes:UP000249713}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AsT-22 {ECO:0000313|EMBL:RAI52086.1,
RC   ECO:0000313|Proteomes:UP000249713};
RA   Zhirakovskaya E.;
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00034618};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RAI52086.1}.
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DR   EMBL; QLIW01000015; RAI52086.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A327LNU0; -.
DR   OrthoDB; 5298826at2; -.
DR   Proteomes; UP000249713; Unassembled WGS sequence.
DR   GO; GO:0005694; C:chromosome; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   GO; GO:0003916; F:DNA topoisomerase activity; IEA:InterPro.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   Gene3D; 3.40.91.30; -; 1.
DR   Gene3D; 3.30.65.10; Bacterial Topoisomerase I, domain 1; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR013498; Topo_IA_Znf.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR   PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR   Pfam; PF00580; UvrD-helicase; 2.
DR   Pfam; PF13361; UvrD_C; 1.
DR   Pfam; PF01396; zf-C4_Topoisom; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   SUPFAM; SSF57783; Zinc beta-ribbon; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000249713}.
FT   DOMAIN          199..672
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51198"
FT   BINDING         220..227
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ   SEQUENCE   969 AA;  108339 MW;  094867982D604E50 CRC64;
     MNRLQQKPLV GFFLNPFGKH AKYIEYDGNE IVIQAKPLVS GTLRALASAP TVESGFWGAS
     LTLRLDDQPV HLLKGARAKD AINFSNTIRS DWEATNRADF DQSRAAIDAL VLEIGELANP
     VIYPAACVVA LILERARGLD QRLLSKLPQE ALEDAHRDQI KKIQSFIQGA RQIRQQAVSA
     FEERQLVEWA EFFETFESNP LTLEQRQSIV SDEDATLVLA GAGSGKTSVI TAKAGYLLKS
     GSRKPEEVLL LAFAKDAAKE MSERIEAKCG EPLEARTFHS LAYDIIGTVE GSKPALAAHA
     TDDKAYLALI RDILRALVRT ASEVSKSIVG WFSYTRLEEK SEWDFKKKHS YYTYIEKLDL
     RTLQGEQVKS FEELMIANWL YENGIEYEYE PDYEHKVSEG GFRDYRPDFR LTKSGIYIEH
     FGVRRTKSAD GTYRLSTAPF VDREEYLAGM DWKRGVHAEH ETTLIETFSY ERKDGLLLEA
     LAEKVSPYEA INPRSPETLF DRVVELNQAD GFVQLLGTFL KHYKGGGYQL SECAEKGRAL
     KLGKRAKAFL SIFEPVYLEY QKQLNGRIDF EDMILRATKY AETGEYVSPF KHILVDEFQD
     ISRSRGCLVK ALKAQHKDAR VFAVGDDWQS IYRFAGSDIN LMRNFGDEFG GVFDGQSGVH
     RTVDLGRTFR SVDQIAHAAK RFVLQNPAQL NKTVIPAGKA DVPALKVVST IRADEKSKLE
     QVLHSLQRTA QPNQPASVLL LGRYRHLAPS NLSQLRRKFS DLDISFRTIH ASKGLEADHV
     VILNMFRGRT GFPSEIVDDP LLSLVSPEVE PFENAEERRV MYVALTRARK SVTLMGSASK
     QSAFVTELLE DPEYGTTGAE GQVEFNHTCG ECGGHLVPVP TKDGRTWYRC KHAILCGHSL
     NACASCGVGL PVRKKSTGLS KCSCGAEYPS CPSCEDGWLV ERRGRYGPFL GCVSYPHCKG
     KAKIAKSKG
//