ID A0A327W7U3_9ACTN Unreviewed; 165 AA.
AC A0A327W7U3;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=Transcription elongation factor GreA {ECO:0000256|ARBA:ARBA00013729, ECO:0000256|HAMAP-Rule:MF_00105};
DE AltName: Full=Transcript cleavage factor GreA {ECO:0000256|HAMAP-Rule:MF_00105};
GN Name=greA {ECO:0000256|HAMAP-Rule:MF_00105};
GN ORFNames=K377_03303 {ECO:0000313|EMBL:RAJ86455.1};
OS Streptomyces sp. PsTaAH-137.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1305830 {ECO:0000313|EMBL:RAJ86455.1, ECO:0000313|Proteomes:UP000249376};
RN [1] {ECO:0000313|EMBL:RAJ86455.1, ECO:0000313|Proteomes:UP000249376}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PsTaAH-137 {ECO:0000313|EMBL:RAJ86455.1,
RC ECO:0000313|Proteomes:UP000249376};
RA Currie C.;
RT "Biomass-degrading enzyme discovery via genome sequencing of insect-
RT associated Streptomyces.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreA releases sequences of 2 to 3 nucleotides.
CC {ECO:0000256|ARBA:ARBA00024916, ECO:0000256|HAMAP-Rule:MF_00105,
CC ECO:0000256|RuleBase:RU000556}.
CC -!- SIMILARITY: Belongs to the GreA/GreB family.
CC {ECO:0000256|ARBA:ARBA00008213, ECO:0000256|HAMAP-Rule:MF_00105,
CC ECO:0000256|RuleBase:RU000556}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAJ86455.1}.
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DR EMBL; QLLY01000006; RAJ86455.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A327W7U3; -.
DR OrthoDB; 9797227at2; -.
DR Proteomes; UP000249376; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0032784; P:regulation of DNA-templated transcription elongation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.50.30; Transcription elongation factor, GreA/GreB, C-terminal domain; 1.
DR Gene3D; 1.10.287.180; Transcription elongation factor, GreA/GreB, N-terminal domain; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR NCBIfam; TIGR01462; greA; 1.
DR PANTHER; PTHR30437; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR PANTHER; PTHR30437:SF4; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR PIRSF; PIRSF006092; GreA_GreB; 1.
DR SUPFAM; SSF54534; FKBP-like; 1.
DR SUPFAM; SSF46557; GreA transcript cleavage protein, N-terminal domain; 1.
DR PROSITE; PS00829; GREAB_1; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00105}; Elongation factor {ECO:0000313|EMBL:RAJ86455.1};
KW Protein biosynthesis {ECO:0000313|EMBL:RAJ86455.1};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00105};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00105}.
FT DOMAIN 10..79
FT /note="Transcription elongation factor GreA/GreB N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF03449"
FT DOMAIN 86..161
FT /note="Transcription elongation factor GreA/GreB C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01272"
FT REGION 43..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 165 AA; 17841 MW; 05FB3B258D0E7009 CRC64;
MTQTSEDVTW LTQEAHTKLK DELAYLSGPA REEVTQKIAA AREEGDLREN GGYHAAKEEQ
GKQELRIRQL TQLLEKAKVG EAPAASGVVA PGMVVTIAFD GDEDDTLEFL LASREYASSD
IETYSPQSPL GTGVNGKKVG EDAEYELPNG KLASVKILKA EPYSG
//