ID A0A327YG84_9BACI Unreviewed; 485 AA.
AC A0A327YG84;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=B0I26_10753 {ECO:0000313|EMBL:RAK19136.1};
OS Anoxybacillus vitaminiphilus.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Anoxybacillus.
OX NCBI_TaxID=581036 {ECO:0000313|EMBL:RAK19136.1, ECO:0000313|Proteomes:UP000248555};
RN [1] {ECO:0000313|EMBL:RAK19136.1, ECO:0000313|Proteomes:UP000248555}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.8979 {ECO:0000313|EMBL:RAK19136.1,
RC ECO:0000313|Proteomes:UP000248555};
RA Whitman W.;
RT "Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the genomes of
RT soil and plant-associated and newly described type strains.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAK19136.1}.
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DR EMBL; QLMH01000007; RAK19136.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A327YG84; -.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000248555; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..129
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 137..451
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 485 AA; 55686 MW; B06C5DF524FDDC4B CRC64;
MLTGELRNKV DKIWETFWTG GITNPLTVIE QFTYLLFIKG LDEVETKNEQ EAALLGIDFE
RIFPEDKQHL RWSKFKNLEA TQMYEIVSNE VFPFIKNLHD NKDSAYAKYM GDAIFMIPTP
QMLTKIVDGI DNIPMKDRDT KGDLYEYLLS KIATAGTNGQ FRTPRHIIKM MVELVKPTPE
DIIVDPAAGS AGFLVAAGEY LRKYRSDLFL VQSLKEHFNN DMFYGFDMDR TMLRIGAMNM
MLHGIENPNI EYRDSLSEQN KDKDKYTLVL ANPPFKGSLD YEAVSSDLLK ITKTKKTELL
FLALFLRILK TGGRCACIVP DGVLFGSSKA HKDIRKEIVE NHKLEAIISM PSGVFKPYAG
VSTAIMIFTK TGVGGTDQVW FYDMKADGYS LDDKRTPIEE NDIPDIIARF HNREAEKDRK
RTEQSFFVPV EEIRENDYDL SINKYKEIEY EEVQYEAPSV ILERVEALEK EIMNGLEELK
KIIKG
//
