ID A0A327YWR2_9ACTN Unreviewed; 659 AA.
AC A0A327YWR2;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=cellulase {ECO:0000256|ARBA:ARBA00012601};
DE EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601};
GN ORFNames=B0I29_13122 {ECO:0000313|EMBL:RAK25671.1};
OS Actinoplanes lutulentus.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Actinoplanes.
OX NCBI_TaxID=1287878 {ECO:0000313|EMBL:RAK25671.1, ECO:0000313|Proteomes:UP000249341};
RN [1] {ECO:0000313|EMBL:RAK25671.1, ECO:0000313|Proteomes:UP000249341}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 4.7090 {ECO:0000313|EMBL:RAK25671.1,
RC ECO:0000313|Proteomes:UP000249341};
RA Whitman W.;
RT "Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the genomes of
RT soil and plant-associated and newly described type strains.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAK25671.1}.
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DR EMBL; QLMJ01000031; RAK25671.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A327YWR2; -.
DR OrthoDB; 4902692at2; -.
DR Proteomes; UP000249341; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030247; F:polysaccharide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.290; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR001919; CBD2.
DR InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR InterPro; IPR012291; CBM2_carb-bd_dom_sf.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR018087; Glyco_hydro_5_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR35923:SF2; CELLULASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR35923; MAJOR EXTRACELLULAR ENDOGLUCANASE; 1.
DR Pfam; PF00553; CBM_2; 1.
DR Pfam; PF00150; Cellulase; 1.
DR Pfam; PF00041; fn3; 1.
DR SMART; SM00637; CBD_II; 1.
DR SMART; SM00060; FN3; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49384; Carbohydrate-binding domain; 1.
DR SUPFAM; SSF49265; Fibronectin type III; 1.
DR PROSITE; PS51173; CBM2; 1.
DR PROSITE; PS50853; FN3; 1.
DR PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE 4: Predicted;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326};
KW Reference proteome {ECO:0000313|Proteomes:UP000249341};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 27..659
FT /note="cellulase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039574668"
FT DOMAIN 471..556
FT /note="Fibronectin type-III"
FT /evidence="ECO:0000259|PROSITE:PS50853"
FT DOMAIN 558..659
FT /note="CBM2"
FT /evidence="ECO:0000259|PROSITE:PS51173"
SQ SEQUENCE 659 AA; 70423 MW; CCB34CFCE72E079F CRC64;
MFQRRGRKRL AAAVAAVALA LTGGQAFPAA APAAPAAASA ALQATAFEAA AAPGSDWLHV
DGNQIVDAAG NPVWLTGVNW FGFNASERVF HGLWSANITE ITRSMAQRGI NIVRVPISTQ
LLLEWKAGTF GTVNVNTYAN PELTGKNSLQ IFDYWLALCE QYGIKVLLDV HSAEADNSGH
IHPVWYKGTI TPELYYQAWE WVTARYKAND TIVAMDLKNE PHGTPGTTPR AKWDNSTDVD
NWKYTAETAA KRILAINPEV LVLVEGQEVY PREGETWSSP NTDPDLSPNY YYNWWGGNLR
GVRDYPINLG ANQDQLVYSP HDYGPLVFNQ PWFDKPFDKT SLTNDVWRPN WLYIHENDTA
PLLIGEWGGR LGQDERQDRW MTALRDLIVE HRLHQTFWVL NPNSGDTGGL LLDDWKTWDE
QKYALLKPAL WQQSGKFVSL DHEVALGGAG STTGISLSAA YGSADTVAPS VPGQPASSSI
TSTGVTLTWA ASTDNVGVTS YDVLRGGSVV ATVSGTTYNA TGLTANTAYS FTVRARDAAG
NVSAASTARS VTTAASGGGG GDGGCTATYR TTSSWSGGFQ AEVTVRNTGT AAGTSWVVNW
TYPSGTTVAS LWNGVQGTPA VTVRNADHNG ALAGGASAAF GLVGTGTAAT PATVSCTLS
//
