ID A0A328RVG2_9EURY Unreviewed; 316 AA.
AC A0A328RVG2;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 13.
DE RecName: Full=Tyrosine recombinase XerA {ECO:0000256|HAMAP-Rule:MF_02055};
GN Name=xerA {ECO:0000256|HAMAP-Rule:MF_02055};
GN ORFNames=BZ134_05735 {ECO:0000313|EMBL:RAP43795.1};
OS Methanosphaera sp. SHI1033.
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX NCBI_TaxID=1945632 {ECO:0000313|EMBL:RAP43795.1, ECO:0000313|Proteomes:UP000249059};
RN [1] {ECO:0000313|Proteomes:UP000249059}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Shi W., Moon C.D., Leahy S.C., Kang D., Froula J., Kittlemann S., Fan C.,
RA Deutsch S., Sang C., Soni P., Li D., Pinares-Patino C.S., Mcewan J.C.,
RA Janeesn P.H., Chen F., Visel A., Wang Z., Attwood G.T., Rubin E.M.;
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:RAP43795.1, ECO:0000313|Proteomes:UP000249059}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SHI1033 {ECO:0000313|EMBL:RAP43795.1};
RA Hoedt E.C., Parks D.H., Rosewarne C.P., Denman S.E., Mcsweeney C.S.,
RA O Cuiv P., Hugenholtz P., Tyson G.W., Morrison M.;
RT "Culture- and metagenomics-based expansion of the genus Methanosphaera
RT reveals their evolutionary depth and metabolic versatility.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules.
CC {ECO:0000256|HAMAP-Rule:MF_02055}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_02055}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerA subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_02055}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAP43795.1}.
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DR EMBL; MVJK01000044; RAP43795.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A328RVG2; -.
DR Proteomes; UP000249059; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006313; P:DNA transposition; IEA:UniProtKB-UniRule.
DR CDD; cd00798; INT_XerDC_C; 1.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; Intergrase catalytic core; 1.
DR HAMAP; MF_02055; Recomb_XerA; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR025269; SAM-like_dom.
DR InterPro; IPR033686; XerA.
DR PANTHER; PTHR30349:SF41; INTEGRASE_RECOMBINASE HI_1414-RELATED; 1.
DR PANTHER; PTHR30349; PHAGE INTEGRASE-RELATED; 1.
DR Pfam; PF13102; Phage_int_SAM_5; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_02055};
KW DNA integration {ECO:0000256|ARBA:ARBA00022908, ECO:0000256|HAMAP-
KW Rule:MF_02055};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP-
KW Rule:MF_02055};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_02055}.
FT DOMAIN 9..95
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000259|PROSITE:PS51900"
FT DOMAIN 110..296
FT /note="Tyr recombinase"
FT /evidence="ECO:0000259|PROSITE:PS51898"
FT ACT_SITE 159
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 183
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 248
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 251
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 274
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 283
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
SQ SEQUENCE 316 AA; 37314 MW; 0DAE4CCC6292F3B1 CRC64;
MTSEYLEELE LEDQIEDYIL ELDIQSYSTN TLKTYKSILN SFHRFLLSQK KLNSPKDMLR
SFKRYLQYLK REKEVSQNYL YLVTVVLKKF FEYAEIPIYD EIKAPKRTKS LPKSLNEQEV
YDLIHARDAD YDPKKSNPQN ITRLRNKVIL TLLYSTGLRV SELVNLKIRA IDEDERTIRV
RGKGEKDRIV IFDDTTLNLI NEYLDKRGVE NEYLFVNQKN NKLTSRYIEL MIKDHAKRAG
INKRVTPHIL RHSFATHLLK NGVDIRAIQQ LLGHSNLSTT QIYTSVDMHT LKNVYDEAWL
NRDHNVHKTP IDSTTE
//
