ID A0A328S0H8_9EURY Unreviewed; 748 AA.
AC A0A328S0H8;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=Hef nuclease {ECO:0000313|EMBL:RAP45713.1};
GN ORFNames=BZ135_04970 {ECO:0000313|EMBL:RAP45713.1};
OS Methanosphaera sp. rholeuAM6.
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX NCBI_TaxID=1945580 {ECO:0000313|EMBL:RAP45713.1, ECO:0000313|Proteomes:UP000248984};
RN [1] {ECO:0000313|EMBL:RAP45713.1, ECO:0000313|Proteomes:UP000248984}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RholeuAM6 {ECO:0000313|EMBL:RAP45713.1};
RA Hoedt E.C., Parks D.H., Rosewarne C.P., Denman S.E., Mcsweeney C.S.,
RA O Cuiv P., Hugenholtz P., Tyson G.W., Morrison M.;
RT "Culture- and metagenomics-based expansion of the genus Methanosphaera
RT reveals their evolutionary depth and metabolic versatility.";
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAP45713.1}.
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DR EMBL; MVAA01000076; RAP45713.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A328S0H8; -.
DR Proteomes; UP000248984; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR CDD; cd09897; H3TH_FEN1-XPG-like; 1.
DR CDD; cd12089; Hef_ID; 1.
DR CDD; cd20075; XPF_nuclease_XPF_arch; 1.
DR Gene3D; 3.40.50.10130; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 1.20.1320.20; hef helicase domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR006166; ERCC4_domain.
DR InterPro; IPR041755; Hef_ID.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR010994; RuvA_2-like.
DR PANTHER; PTHR14025; FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER; 1.
DR PANTHER; PTHR14025:SF20; FANCONI ANEMIA GROUP M PROTEIN; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF02732; ERCC4; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF14520; HHH_5; 1.
DR Pfam; PF21210; RNA_helicase_helical; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00891; ERCC4; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00278; HhH1; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR SUPFAM; SSF47781; RuvA domain 2-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000248984}.
FT DOMAIN 25..190
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 354..516
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 748 AA; 86033 MW; 2159A83B0E6A2783 CRC64;
MNNFIEHEYL RKDTIEGRLY QQNLAINVLK KGNTMIIAPT AMGKTVVAAL VSAERLKNFK
DSKILILAPS KPLTLQHEKS FKTFLNTSVV SLTGNDKPSD RKKLWEENQV ICATPQTVES
DIISREYNFK DVSLIIFDEC HHAVGSYSYV YLAQKYVQQG KNQLILGLTA SPGWEKSKIK
EVSKNIYVNE IIIKSEDDPD VAPYFNQVQT KWIKVKLTPE LQEIKDLINE TLKIRLKTLK
KLGIIDSISK PSKKEVLVEQ SRLQNKIASS SVPKKEYFTG ISILTEVINI MHSQELLETQ
SISTLNNYFK KLEKKKTKAA KSLKNDYKFN KAVMLARKYV DSGVDHPKMI RLVELIQQLI
KEDSENKIIV FSQFRDTTKT IYENCQKNKI KALRFYGQAS RENDKGLTQK KQIETIDSFK
HDDYNVLIST SVAEEGIDIP SVDYVILYEP VPSEIRMIQR KGRTGRKHEG EMFILMTKGT
LDESYYWSSQ RKERAMRNNV YNSYRDESIT LDNHVADNDD VTSYNVNSLT NDDNADVEIY
VDYREKNSNM MRELDKINCK INVKTMSVGD YQITDDIIIE RKTVEDFSKS ITDKRLYQQA
KELVNNCRKP LMIIEGENIY HTFLHPNAIR GALSAIAIDF RIPIIQTHNE TDTAFMIKRI
AMREQDKDTQ KPVSVRTQTK PVTLPEQQLF ITESLPGIGP VSAKKLLQHF KTIRKLITAS
KKELKEVEGI GEKTATNIID VTNREFKE
//
