ID A0A328S590_9EURY Unreviewed; 612 AA.
AC A0A328S590;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Probable translation initiation factor IF-2 {ECO:0000256|HAMAP-Rule:MF_00100};
GN Name=infB {ECO:0000256|HAMAP-Rule:MF_00100};
GN ORFNames=BZ135_02370 {ECO:0000313|EMBL:RAP47343.1};
OS Methanosphaera sp. rholeuAM6.
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX NCBI_TaxID=1945580 {ECO:0000313|EMBL:RAP47343.1, ECO:0000313|Proteomes:UP000248984};
RN [1] {ECO:0000313|EMBL:RAP47343.1, ECO:0000313|Proteomes:UP000248984}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RholeuAM6 {ECO:0000313|EMBL:RAP47343.1};
RA Hoedt E.C., Parks D.H., Rosewarne C.P., Denman S.E., Mcsweeney C.S.,
RA O Cuiv P., Hugenholtz P., Tyson G.W., Morrison M.;
RT "Culture- and metagenomics-based expansion of the genus Methanosphaera
RT reveals their evolutionary depth and metabolic versatility.";
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Function in general translation initiation by promoting the
CC binding of the formylmethionine-tRNA to ribosomes. Seems to function
CC along with eIF-2. {ECO:0000256|ARBA:ARBA00024852, ECO:0000256|HAMAP-
CC Rule:MF_00100, ECO:0000256|RuleBase:RU000644}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000256|ARBA:ARBA00007733, ECO:0000256|HAMAP-Rule:MF_00100,
CC ECO:0000256|RuleBase:RU000644}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAP47343.1}.
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DR EMBL; MVAA01000038; RAP47343.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A328S590; -.
DR Proteomes; UP000248984; Unassembled WGS sequence.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd03703; aeIF5B_II; 1.
DR CDD; cd16266; IF2_aeIF5B_IV; 1.
DR CDD; cd01887; IF2_eIF5B; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR Gene3D; 3.40.50.10050; Translation initiation factor IF- 2, domain 3; 1.
DR HAMAP; MF_00100_A; IF_2_A; 1.
DR InterPro; IPR029459; EFTU-type.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR004544; TF_aIF-2_arc.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR NCBIfam; TIGR00491; aIF-2; 1.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43381:SF4; EUKARYOTIC TRANSLATION INITIATION FACTOR 5B; 1.
DR PANTHER; PTHR43381; TRANSLATION INITIATION FACTOR IF-2-RELATED; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF14578; GTP_EFTU_D4; 1.
DR Pfam; PF11987; IF-2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF52156; Initiation factor IF2/eIF5b, domain 3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW GTP-binding {ECO:0000256|HAMAP-Rule:MF_00100};
KW Initiation factor {ECO:0000256|ARBA:ARBA00022540, ECO:0000256|HAMAP-
KW Rule:MF_00100}; Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00100};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00100}; Reference proteome {ECO:0000313|Proteomes:UP000248984}.
FT DOMAIN 3..220
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
FT BINDING 12..19
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00100"
FT BINDING 76..80
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00100"
FT BINDING 130..133
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00100"
SQ SEQUENCE 612 AA; 68068 MW; 532F6E0C117EF058 CRC64;
MKTRSPIVSV LGHVDHGKTT LLDHIRGSTV ASREAGGITQ HIGATEIPMD DISSICGDFL
EKMKIEDRLP GLFFIDTPGH EAFTTLRKRG GSLADLAILI MDVSEGFKPQ TYEALNILKS
SKTPFVVAAN KIDRIPGWNS TDNASFSEVV QNQHSKVAFD LDQKLYEIVG TLHEEGFESE
RFDRVSNFAS QITIVPISAQ TGEGLPELLT MLLGLAYQYL REQLQIEEDA PASGTVLEVK
EEKGLGLTID TILYDGVLNK NDHIMMLTKD NKVISTKIRS LLKPKALEEI RESKTMFEDA
EQIVAAAGVK IVAPHVDDVI SGSPLKVAND DNLRIEEELL SEVDNIRIQT NDVGIIVKAD
TLGSLEALVN ILDSKDIPIK SAEIGDISRR DIINASIMYE EDPKHGVIVA FNVNILPSAE
EELATQDIQV FQDKVIYQLT EDYMEWVTTA KERQKKERLQ ALTRPSTIRI MPKLVFRNSK
PAIAGVEIMS GIITRGAVLI NDKGNYVGRV ESMEDNGESL AKASRGSKVA MAIADATFEK
DFEEGDVLYV DMDERNFMAV LNELADKLLD DELLTLDELK EIKQQHEDPN WGVINTDWSE
LDTFDDDEVE FY
//
