ID A0A328SSL4_9EURY Unreviewed; 317 AA.
AC A0A328SSL4;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
DE Flags: Fragment;
GN ORFNames=BZ137_07175 {ECO:0000313|EMBL:RAP53259.1};
OS Methanosphaera sp. rholeuAM130.
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX NCBI_TaxID=1945578 {ECO:0000313|EMBL:RAP53259.1, ECO:0000313|Proteomes:UP000248969};
RN [1] {ECO:0000313|EMBL:RAP53259.1, ECO:0000313|Proteomes:UP000248969}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RholeuAM130 {ECO:0000313|EMBL:RAP53259.1};
RA Hoedt E.C., Parks D.H., Rosewarne C.P., Denman S.E., Mcsweeney C.S.,
RA O Cuiv P., Hugenholtz P., Tyson G.W., Morrison M.;
RT "Culture- and metagenomics-based expansion of the genus Methanosphaera
RT reveals their evolutionary depth and metabolic versatility.";
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAP53259.1}.
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DR EMBL; MUZY01000194; RAP53259.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A328SSL4; -.
DR Proteomes; UP000248969; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 2.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279}.
FT DOMAIN 201..317
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT NON_TER 317
FT /evidence="ECO:0000313|EMBL:RAP53259.1"
SQ SEQUENCE 317 AA; 35789 MW; AB31210CE91E7049 CRC64;
MKGIIKKISE IDFGLMSPET IRKMSVTKIV TPDTYDEDGY PIEAGLMDPR LGVIDPGLKC
RTCGSKGGDC QGHFGHINFA RPVIHVGFAD TIHKILRSTC RSCGCVLLSD TEKEKYKDKL
EERIANGEDI SKILKQIHVA AKKEKCPHCD EEQIEIKIDK PISIVEGNYK LTASEVRERL
ENIPEDDYIY VGINKDVARP EWMVLTVLPV PPVTVRPSIT LETGERSEDD LTHKLVDILR
INQRLKENME AGAPQLIVED LWELLQYHVT TYFDNEASGV PPARHRSGRP LKTLTQRLKG
KEGRFRSNLS GKRVNFS
//
