ID A0A329N5N5_9FLAO Unreviewed; 258 AA.
AC A0A329N5N5;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 24-JAN-2024, entry version 12.
DE SubName: Full=HAD family hydrolase {ECO:0000313|EMBL:RAV30091.1};
GN ORFNames=DN748_04650 {ECO:0000313|EMBL:RAV30091.1};
OS Sinomicrobium sp. N-1-3-6.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Sinomicrobium.
OX NCBI_TaxID=2219864 {ECO:0000313|EMBL:RAV30091.1, ECO:0000313|Proteomes:UP000250485};
RN [1] {ECO:0000313|EMBL:RAV30091.1, ECO:0000313|Proteomes:UP000250485}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N-1-3-6 {ECO:0000313|EMBL:RAV30091.1,
RC ECO:0000313|Proteomes:UP000250485};
RA Liu X., Lai Q., Shao Z.;
RT "Sinomicrobium soli sp. nov., isolated from arctic soil.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR000915-3};
CC Note=Divalent metal ions. Mg(2+) is the most effective.
CC {ECO:0000256|PIRSR:PIRSR000915-3};
CC -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily.
CC {ECO:0000256|PIRNR:PIRNR000915}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAV30091.1}.
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DR EMBL; QLNV01000003; RAV30091.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A329N5N5; -.
DR OrthoDB; 9810449at2; -.
DR Proteomes; UP000250485; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07530; HAD_Pase_UmpH-like; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 2.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR006357; HAD-SF_hydro_IIA.
DR InterPro; IPR023214; HAD_sf.
DR NCBIfam; TIGR01460; HAD-SF-IIA; 1.
DR PANTHER; PTHR19288; 4-NITROPHENYLPHOSPHATASE-RELATED; 1.
DR PANTHER; PTHR19288:SF46; HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2; 1.
DR Pfam; PF13344; Hydrolase_6; 1.
DR Pfam; PF13242; Hydrolase_like; 1.
DR PIRSF; PIRSF000915; PGP-type_phosphatase; 2.
DR SUPFAM; SSF56784; HAD-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000313|EMBL:RAV30091.1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR000915-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000915-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000250485}.
FT ACT_SITE 8
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-1"
FT ACT_SITE 10
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-1"
FT BINDING 8
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-3"
FT BINDING 10
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-3"
FT BINDING 179
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-2"
FT BINDING 204
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR000915-3"
SQ SEQUENCE 258 AA; 28226 MW; A6DFCFB7E6B16E6A CRC64;
MKKGFLIDMD GVIYSGDTMI EGADTFIAAL QEQKIPFLFM TNNSQRSPLD TVNKVAKMGI
RIKEENVYTS AMATAWFLSF MKPNGTVYVL GEGGLITSLH QQGYVMVNQN PDYVVVGEGR
NFTLEMVNNA VDMILSGAKF IATNLDPSPK KEGWNNLGIK AVVAMIEEAT GKKAFSVGKP
SPVMMRAARK TLGLTAQETI IIGDTMDTDI LGGVQLGYKT ILTLSGVSKK TDLKHYAFQP
NKIINSVKDL SLEELKEL
//
