ID A0A329S6G2_9STRA Unreviewed; 716 AA.
AC A0A329S6G2;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=JG687_00007697 {ECO:0000313|EMBL:KAG6961470.1}, PC110_g12375
GN {ECO:0000313|EMBL:RAW31268.1};
OS Phytophthora cactorum.
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=29920 {ECO:0000313|EMBL:RAW31268.1, ECO:0000313|Proteomes:UP000251314};
RN [1] {ECO:0000313|EMBL:RAW31268.1, ECO:0000313|Proteomes:UP000251314}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=10300 {ECO:0000313|EMBL:RAW31268.1,
RC ECO:0000313|Proteomes:UP000251314};
RA Armitage A.D., Lysoe E., Nellist C.F., Harrison R.J., Brurberg M.B.;
RT "Draft genome of the strawberry crown rot pathogen Phytophthora cactorum.";
RL Submitted (JAN-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAG6961470.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NZFS 3830 {ECO:0000313|EMBL:KAG6961470.1};
RA Mcdougal R., Panda P., Williams N., Studholme D.J.;
RT "Phytophthora aleatoria, a newly-described species from Pinus radiata is
RT distinct from Phytophthora cactorum isolates based on comparative
RT genomics.";
RL Submitted (JAN-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAW31268.1}.
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DR EMBL; JAENGZ010000348; KAG6961470.1; -; Genomic_DNA.
DR EMBL; MJFZ01000330; RAW31268.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A329S6G2; -.
DR STRING; 29920.A0A329S6G2; -.
DR Proteomes; UP000251314; Unassembled WGS sequence.
DR Proteomes; UP000688947; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000251314};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT TRANSMEM 6..25
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 379..716
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 204..260
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 683
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 716 AA; 81211 MW; 461D88736C4E95A7 CRC64;
MSVGGTIAVA CALALFLVMC FGWLARRSFK KFEREAELNA PLIDSEDKRL DAALRDAEEG
YNACAVCEFE NFKRFRFCAI CGELMRKGDD EGDGEGDAEK KKPSLKFSLL SRFRRDSHVQ
LAAPASASTL TNQVTRRCTR AQRRKEWTRK MDVEGRMYWF RDSRFVKTDA LPAAFVAKFE
PASTAAIMVD KFFWSQLLPS IQDSGRLPRS TTESQEQPKE DMITSGTHSS NHADNASSTP
STPEPEPELD NQTPQRSTFG SLERRMASLK KSVQKMELVL VPASDAALDA SKLPLTISVG
PKLTTSYAAQ CDAPERIEMP KSVTEAMAFA NQDFPSKYAQ FVKTASSLLV PAEREHLRLN
VERAKVFKDS MEPLVVIPLR NLHSVMRINF VDESGIDAGG LQREWFVMLN EALVDPAHRL
FVCTNKKEQT YSLRPCWNVD DLPRQLLYLL AAGRLLGRAL VEGSMLCFHL APPLLKLILG
YPLSFSDLED FDPEVYTNLR WLLENENVDS LGLDFTMTVK DGDTYRTIEL VPGGKGIAIS
DSNKREYVER KWQYLLVESI APQLQVFLRG LYEIIPRELL LLFDPEEFDF LLCGSEEIDV
DDWERNTKYS EGLHHHRVLK WFWEFVREMP NEYRRRLLQF ATGSSRVPLG GFSALTSYDG
RLCPFTLKVV QLVGDGFIHS HACFNRLDLP LHVAREELKA VLYAMLETEA YGFSTD
//
