ID A0A340WZM8_LIPVE Unreviewed; 455 AA.
AC A0A340WZM8;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Bleomycin hydrolase {ECO:0000256|ARBA:ARBA00022227, ECO:0000256|PIRNR:PIRNR005700};
DE EC=3.4.22.40 {ECO:0000256|ARBA:ARBA00012465, ECO:0000256|PIRNR:PIRNR005700};
GN Name=BLMH {ECO:0000313|RefSeq:XP_007452519.1};
OS Lipotes vexillifer (Yangtze river dolphin).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Whippomorpha; Cetacea; Odontoceti;
OC Lipotidae; Lipotes.
OX NCBI_TaxID=118797 {ECO:0000313|Proteomes:UP000265300, ECO:0000313|RefSeq:XP_007452519.1};
RN [1] {ECO:0000313|RefSeq:XP_007452519.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by
CC hydrolysis of a carboxyamide bond of beta-aminoalanine, but also
CC shows general aminopeptidase activity. The specificity varies
CC somewhat with source, but amino acid arylamides of Met, Leu and Ala
CC are preferred.; EC=3.4.22.40;
CC Evidence={ECO:0000256|ARBA:ARBA00000423,
CC ECO:0000256|PIRNR:PIRNR005700};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR005700}.
CC -!- SIMILARITY: Belongs to the peptidase C1 family.
CC {ECO:0000256|PIRNR:PIRNR005700}.
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DR RefSeq; XP_007452519.1; XM_007452457.1.
DR AlphaFoldDB; A0A340WZM8; -.
DR GeneID; 103089598; -.
DR CTD; 642; -.
DR OrthoDB; 45184at2759; -.
DR Proteomes; UP000265300; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0070005; F:cysteine-type aminopeptidase activity; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00585; Peptidase_C1B; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR004134; Peptidase_C1B.
DR PANTHER; PTHR10363; BLEOMYCIN HYDROLASE; 1.
DR PANTHER; PTHR10363:SF2; BLEOMYCIN HYDROLASE; 1.
DR Pfam; PF03051; Peptidase_C1_2; 1.
DR PIRSF; PIRSF005700; PepC; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR005700};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005700};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PIRNR:PIRNR005700};
KW Reference proteome {ECO:0000313|Proteomes:UP000265300};
KW Thiol protease {ECO:0000256|ARBA:ARBA00022807,
KW ECO:0000256|PIRNR:PIRNR005700}.
FT ACT_SITE 73
FT /evidence="ECO:0000256|PIRSR:PIRSR005700-1"
FT ACT_SITE 372
FT /evidence="ECO:0000256|PIRSR:PIRSR005700-1"
FT ACT_SITE 396
FT /evidence="ECO:0000256|PIRSR:PIRSR005700-1"
SQ SEQUENCE 455 AA; 52320 MW; E6FBE9D090FA7047 CRC64;
MSNSGLNSEK VAALLQKLNS DPQFVLAQNV GTTHDLLDIC LKRASVQGVQ HVFQHAVHPE
GKPVTNQKSS GRCWIFSCLN VMRLPFMRKL NIEEFEFSQS YLFFWDKVER CYFFLNAFVD
TAQKKEPEDG RLVQYLLTNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATRRMNDI
LNHKMREFCI RLRNLVHSGA TKGEISATQD AMMEEIFRVV CICLGNPPET FTWEYRDKDK
NYQKIGPITP LKFYKEHVKP LFNMEDKICL VNDPRPHHKY SKLYTVDYLS NMVGGRKTLY
NNQPIDLLKK MVAASIKDGE AVWFGCDVGK HFSGKLGLSD MNVYDHELVF GVSMKNMNKA
ERLTFGDSLM THAMTFTAVS EKDDQEGAFV KWRVENSWGE DHGHKGYLCM TDEWFSEYVY
EVVVDRKHVP EEVLAVLEQE PIVLPAWDPM GALAE
//
