ID A0A340YEF7_LIPVE Unreviewed; 554 AA.
AC A0A340YEF7;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Hyaluronidase {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
DE EC=3.2.1.35 {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
GN Name=SPAM1 {ECO:0000313|RefSeq:XP_007471343.1};
OS Lipotes vexillifer (Yangtze river dolphin).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Whippomorpha; Cetacea; Odontoceti;
OC Lipotidae; Lipotes.
OX NCBI_TaxID=118797 {ECO:0000313|Proteomes:UP000265300, ECO:0000313|RefSeq:XP_007471343.1};
RN [1] {ECO:0000313|RefSeq:XP_007471343.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-
CC glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00000251,
CC ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004609};
CC Lipid-anchor, GPI-anchor {ECO:0000256|ARBA:ARBA00004609}. Membrane
CC {ECO:0000256|ARBA:ARBA00004589}; Lipid-anchor, GPI-anchor
CC {ECO:0000256|ARBA:ARBA00004589}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 56 family.
CC {ECO:0000256|ARBA:ARBA00008871, ECO:0000256|PIRNR:PIRNR038193,
CC ECO:0000256|RuleBase:RU610713}.
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DR RefSeq; XP_007471343.1; XM_007471281.1.
DR AlphaFoldDB; A0A340YEF7; -.
DR STRING; 118797.A0A340YEF7; -.
DR GlyCosmos; A0A340YEF7; 1 site, No reported glycans.
DR GeneID; 103082114; -.
DR KEGG; lve:103082114; -.
DR CTD; 6677; -.
DR InParanoid; A0A340YEF7; -.
DR OrthoDB; 5344684at2759; -.
DR Proteomes; UP000265300; Unplaced.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004415; F:hyalurononglucosaminidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IEA:InterPro.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR018155; Hyaluronidase.
DR InterPro; IPR001439; Hyaluronidase_PH20/Hyal5.
DR PANTHER; PTHR11769; HYALURONIDASE; 1.
DR PANTHER; PTHR11769:SF20; HYALURONIDASE PH-20; 1.
DR Pfam; PF01630; Glyco_hydro_56; 1.
DR PIRSF; PIRSF038193; Hyaluronidase; 1.
DR PIRSF; PIRSF500773; Hyaluronidase_PH20_Hyal5; 1.
DR PRINTS; PR00846; GLHYDRLASE56.
DR PRINTS; PR00848; SPERMPH20.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR038193-3};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00022622};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR038193};
KW GPI-anchor {ECO:0000256|ARBA:ARBA00022622};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|ARBA:ARBA00022622};
KW Reference proteome {ECO:0000313|Proteomes:UP000265300}.
FT ACT_SITE 148
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-1"
FT CARBOHYD 369
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-2"
FT DISULFID 60..352
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 224..238
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 377..388
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 382..436
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 438..444
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
SQ SEQUENCE 554 AA; 62496 MW; 470B7B847EA6612F CRC64;
MGVLRLQHIS FGSFVGSSGV PQAVFTFLLV PCCLTLDFRA SPLIANMSFL WTWNAPTDRC
ARRFDMPPDL SLFSLVGNPQ KGATGNFITL FYADRLGYYP HIDERTGRSV YGGIPQMGSL
KKHLDKAKKD ISYYMPIDNV GLAVIDWENW RPTWARNWKP KDAYRDQSIE LVLQQNIQLT
FPEAVKIAKV EFEKAAKSFM QETLKLGKLL RPNHLWGYYL FPDCYNHNYN QPSYNGSCWD
IEKRRNDALD WLWKESTALF PSIYLNSRLK SSPQAALFVR NRVQEAVRMS KVASAKSPLP
VFVYARPVFT DVSLQFLSQG DLVNTIGESV ALGASGIIMW GSLNLSLTRQ SCMNLGNYMK
TTLNPYVINV TLAAKMCSQA LCQERGVCTR KYWNSSNYLH LNPKNFAIQT GKGGKYTVHG
KPTLKDLQQF SKKFYCSCYA NIRCKKRVDI KNIHTINVCI AEDVCVDGFL NLEPGNHSSS
WKEISATTLS NISFSTPTVT VSPCVPGKGL RGCLKVTCSV EPLSNNTQGG CHTVKWKNTF
SQLHIQNKKN ETTY
//
