ID A0A344UAT4_9ACTN Unreviewed; 3100 AA.
AC A0A344UAT4;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Beta-ketoacyl synthase {ECO:0008006|Google:ProtNLM};
GN ORFNames=C0216_31345 {ECO:0000313|EMBL:AXE28005.1};
OS Streptomyces globosus.
OG Plasmid unnamed2 {ECO:0000313|EMBL:AXE28005.1,
OG ECO:0000313|Proteomes:UP000252004}.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=68209 {ECO:0000313|EMBL:AXE28005.1, ECO:0000313|Proteomes:UP000252004};
RN [1] {ECO:0000313|EMBL:AXE28005.1, ECO:0000313|Proteomes:UP000252004}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LZH-48 {ECO:0000313|EMBL:AXE28005.1,
RC ECO:0000313|Proteomes:UP000252004};
RC PLASMID=unnamed2 {ECO:0000313|EMBL:AXE28005.1,
RC ECO:0000313|Proteomes:UP000252004};
RA Ran K., Li Z., Wei S., Dong R.;
RT "Draft genome Sequence of streptomyces globosus LZH-48.";
RL Submitted (JAN-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; CP030864; AXE28005.1; -; Genomic_DNA.
DR KEGG; sgz:C0216_31345; -.
DR OrthoDB; 9778690at2; -.
DR Proteomes; UP000252004; Plasmid unnamed2.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt.
DR CDD; cd08956; KR_3_FAS_SDR_x; 1.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 3.30.70.3290; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 2.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR015083; Polyketide_synth_docking.
DR InterPro; IPR036299; Polyketide_synth_docking_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 2.
DR Pfam; PF08990; Docking; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 2.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 2.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 2.
DR SMART; SM01294; PKS_PP_betabranch; 1.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF101173; Docking domain B of the erythromycin polyketide synthase (DEBS); 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 2.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 2.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 2.
PE 4: Predicted;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Plasmid {ECO:0000313|EMBL:AXE28005.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000252004};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 33..443
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1650..1728
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1747..2169
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3020..3095
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 441..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1253..1272
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2606..2631
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2943..3003
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2973..2997
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3100 AA; 320564 MW; 3AEB32CEBD1A0EBB CRC64;
MTNEDKLRDY LRRVTAELQS TRQRLRDAEE RSAGPVAIVG MACHYPGGVE SPDDLWSLVA
EGRDGITPFP GDRGWDLDTL YHPDPDHPGT TTVREGGFLH GAARFDAGFF GISPKEALHT
DPQQRLILQT SWEAVERAGL DPTALRDSRT GVFTGVMHHD YPGAQGAGSV VSGRVAYHLG
LRGPAITVDT ACSSSLVALH LAVRSLRRGE CDMALVGGAT VMATPAAFVE FSRQRGLAPD
GRCKPFAAGA DGTAWSEGVG VIVVERLATA LAAGRRILAV VRGTAVNSDG ASNGLTAPNG
PAQQRVIRDA LVDAGLTPQQ VDAVEAHGTG TPLGDPVEAQ AVLAAYGRDR DRALRLGSVK
SNLGHTQAAA GIAGIVKMAM AMRHGVLPRT LHLDAPSPYV DWSAGAVELL AEEAPWPAGT
EPRRAAVSSF GVSGTNAHVI LEEPPLPADG RTAGQPDARR PAAAADAGDA RDAADSGNAG
RTEPPLTPQA PPGPLAWTLS ARSDAALRAQ AAALAAFLAD RPQDPRPEDV ALSLATTRAS
HEHRAAVVGG DRAGLLAALD DLAAGRPSPA VVEGVTGPAG GVAFLFPGQG SQWRDMAVGL
LDTNEVFRER IAQCERALAP HVDFRLTAVL RGEQDLDRVD VVQPALFAVM VSLAEVWRSL
GIHPAAVAGH SQGEIAAACV AGALTLEDAA RVVALRSRAL TALTGRGGMM SLALGADEAR
DRLRRWDGRL SLAAVNGAAS VVVSGDTDAL DELLAACEED GVRARRVDVD YASHSAHVEA
VRTQILQALA PLRPLAPAIP FFSSVEGRWI EDADAFDADY WYRNLRQTVR FDEAVTALVR
RDCTTLLEVS PHPVVTTGAQ ETVDALGTDT AVAHTLRRDE GGPEQLLHAV ARLYVKGVRP
DWSALWPGAR PTDLPTTAFL TEPYWIVPGP GAGGAGALGL DEVDHPVLRA ALPAADGLVL
TGRLGLASQP WLADHTVEGQ VVLPGAALAE LASRAADEAG CAAVDQLTLL TPVVIPGTGD
IRLRVDVAAP AEDGSRALTL HTLAGAGHWT THATGVLRPD AAEPAAAATE WPPADAEPQD
VEAVYATLAD AGLDYGPAFR GLRALWRGRD GDLHAEVALP DAAEAGAFTL HPALLDACLH
PVALGGFPTT GDGRRPALPF EWQGLRLHAA GAEAVRVTLS AAGPDAVRLT LTDTTGAPVA
SVDTLVLREL PAGALGRPDR GDVLRPDRVT LPLPAGTHED YAVRDPELAR KLGKEPTGEP
ARHVLVPAPT GEPGEAVRGA LTLVQDWLSD DTRADSTLVL VTRNADTDVA EAAVAGLVRS
ARLEHPGSFR ILDLADLDAA TLAAIPAALA ADEPQLTLAS GTATATRLVR GLPAPAATGE
LGPTLVTGAS GTLGQLVARH LVHAHGVRDL VLAGRRGGGA PGMAALVEEL AGAGAKVRAV
ACDISDRAGL AALTAGEHFR TVVHAAGVLD DGLVTGLTPE RVDRVLAPKL DAALHLHELL
PQARLVLFSS AVGILGGLGQ ANYAAANAGL DALSRRRHAA GLPGTSLAWG LWDATSGMTD
GVDRDRLGRS GFRPLPTADA LAALDAALAS DEPVLAPIRL DPGALRAQGE DVAAPLRGLA
GKPARRSAAP GGAAAVSELA ARLAGKSGPE RLRTLLDLVV GHTAAVLGHA SPEAVEPGRA
FKDAGFGSLA AVELRNRLAA ATGLRLPATL VFSHPSPAAV ARHLDGLLAG TAPAPAHAVR
AAAADEDDPV VLVGLGCRLP GGVRTPDDLW EMLVRERDGI GPFPDDRGWP LERLYDPDPA
TPGTASTRHG GFMADATVFD AAFFGIAPRE AVAMDPQQRI LLETVWETCE DAGIDPDSLR
GSRTGVFVGA MYQDYGRLLE NAAAEGFLAP GVGGGVLSGR VAYTYGLEGP TVTVDTACSS
SLTALHLAAQ AVRAGECDLA FAGGVTVLST PVPFVEFSRQ RGLAADGRCK PFAAAADGTA
LAEGAAVVLI ERLSRARAQG HEVLAVLRGS ALNSDGASNG LTAPNGTAQE NVMRAALAAA
GLRPADVAAV EAHGTGTALG DPIEAQALLA VYGQDRPAPL LLGSLKSNVG HVQAAAGAAG
VIKAVLSIRN GLLPRSLHID EPSPHVDWAS GDIALLDRAR PWPEGTAPRR MGVSSFGISG
TNVHVLIEQA PAPEDTPAPL PPLDGVPWLL SARSEQALRE QARRLLDSDT ADVPAVARAL
STTRSAHRHR AMIGGAGPEH KRAALAALAS GGPADGLHLA RATGRRLTLL FTGQGSQLPA
MGRGLYEAFP VYAQAFDEVS AQFRLGTPLR EAVFERDDLL DRTEYTQAAL FAVQVAQFQL
LSSWGVTPDL LIGHSVGELS AACVSGLMTL ADAAALVTAR GRVLGRLPAG GAMVAVRATE
EETLAVLEGR TDRVGIAAVN GPRSVVLSGD EEEVLSAARE LAERGHRTRR LRVDVAFHSP
RVDTALAEFR AAAAEVRFGR PAVPVVSTVR TAHAMDTPDY WADQLRGCVR FLDAVREAHG
SGATAFLELG PDAVLAPAAE SCLPGLPVRP VSLLRRDRDD VRTAAEALGR LHTHGVEVDW
QAVHAGRPAT PVRLPKYAFQ GRRHWPEQPA AAQAAPAPAP VPEAQAQPAE GAPALAPLWS
AVESADLGTA LDLLDLRGDE TPAQVLHALG RLRSGVTAPV LEAARWTRVP DGPAPGLAHD
IVLLAPSTGG EELADAVAGT LVRHGARVRS GGAGRPDLVL ALPGTEPAAE PGVPRWALVD
GDGAVPACDH TVILPKDLAA PARRRLCAAV AGGYREARID PDGLFVRAYE PLVRTGVWRP
EGDVLVAGPA DALTAAIVDA VLASGARCLV AGGADRPTGA LAFGDVAEAA TLTAAIVRAG
SGFEDVRAPL VAVVSLGETV QEAAGSLAAA VHPGLDPRAA VAGLWRAMST GETAVRLLDT
APASPAAGPA AATAERAGAV PEPAETATAT AEPAPPAPVP EPEPEPKSVP TPEPEPDGSQ
PRDSALRATL AELPRTEWAE AVLGGVRGLA AAVLGYESAS EIDAEGEFLD MGLTSVTALE
LRDHLTGLTG LEWPADLLYE YPTPRELADA VADRLTAPAA
//
