ID A0A345HN41_9ACTN Unreviewed; 470 AA.
AC A0A345HN41;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=NAD-dependent malic enzyme {ECO:0000313|EMBL:AXG78115.1};
GN ORFNames=DVK44_10810 {ECO:0000313|EMBL:AXG78115.1};
OS Streptomyces paludis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=2282738 {ECO:0000313|EMBL:AXG78115.1, ECO:0000313|Proteomes:UP000253868};
RN [1] {ECO:0000313|Proteomes:UP000253868}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GSSD-12 {ECO:0000313|Proteomes:UP000253868};
RA Zhao J.;
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR000106-3};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|PIRSR:PIRSR000106-3};
CC Note=Divalent metal cations. Prefers magnesium or manganese.
CC {ECO:0000256|PIRSR:PIRSR000106-3};
CC -!- SIMILARITY: Belongs to the malic enzymes family.
CC {ECO:0000256|ARBA:ARBA00008785, ECO:0000256|RuleBase:RU003427}.
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DR EMBL; CP031194; AXG78115.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A345HN41; -.
DR KEGG; spad:DVK44_10810; -.
DR OrthoDB; 9805787at2; -.
DR Proteomes; UP000253868; Chromosome.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR001891; Malic_OxRdtase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR PIRSF; PIRSF000106; ME; 1.
DR PRINTS; PR00072; MALOXRDTASE.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS51671; ACT; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000106-3,
KW ECO:0000256|RuleBase:RU003427};
KW Reference proteome {ECO:0000313|Proteomes:UP000253868}.
FT DOMAIN 12..86
FT /note="ACT"
FT /evidence="ECO:0000259|PROSITE:PS51671"
FT ACT_SITE 114
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000106-1"
FT ACT_SITE 169
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000106-1"
FT BINDING 211
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR000106-3"
FT BINDING 212
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR000106-3"
FT BINDING 237
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR000106-3"
FT BINDING 397
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000106-2"
SQ SEQUENCE 470 AA; 48546 MW; 1415D47B264B4AFE CRC64;
MATAPSVSYS MTVRLEVPAS GTAVSQLTTA VESSGGSVTG LDVTASGHEK LRIDVTIAAS
STGHAEEIVE QLRGIEGVTL GKVSDRTFLM HLGGKIEMQS KHPIRNRDDL SMVYTPGVAR
VCMAIAENPE DARRLTIKRN SVAVVTDGSA VLGLGNIGPM AALPVMEGKA ALFKRFAGID
AWPICLDTQD SDEIVAIVKA IAPGFAGINL EDISAPRCFE IEARLREALD IPVFHDDQHG
TAIVVLAALT NALRVVGKGI GDVRVVMSGA GAAGTAILKL LLAAGVKHAV VADIHGVVHA
DRADLVDAAP ESPLRWIADN TNPEGVTGTL KEAVVDADVF IGVSAPNVLD GADVAAMADG
AIVFALANPD PEVDPAIARE TAAVVATGRS DFPNQINNVL VFPGVFRGLL DAQSRTVNTD
MMLAAAAALA NVVGEDELNA NYIVPSVFND RVAGAVAGAV REAAKANGEG
//
