ID   A0A345WKN8_9SPHN        Unreviewed;       361 AA.
AC   A0A345WKN8;
DT   07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT   07-NOV-2018, sequence version 1.
DT   27-MAR-2024, entry version 15.
DE   RecName: Full=protein-glutamate methylesterase {ECO:0000256|ARBA:ARBA00039140};
DE            EC=3.1.1.61 {ECO:0000256|ARBA:ARBA00039140};
GN   ORFNames=DM480_00745 {ECO:0000313|EMBL:AXJ94240.1};
OS   Sphingomonas sp. FARSPH.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingomonas.
OX   NCBI_TaxID=2219696 {ECO:0000313|EMBL:AXJ94240.1, ECO:0000313|Proteomes:UP000254856};
RN   [1] {ECO:0000313|EMBL:AXJ94240.1, ECO:0000313|Proteomes:UP000254856}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FARSPH {ECO:0000313|EMBL:AXJ94240.1,
RC   ECO:0000313|Proteomes:UP000254856};
RA   Bendezu Eguis J., Morales Ruiz S., Montesinos R., Quispe Conislla J.,
RA   Tataje-Lavanda L., Fernandez-Sanchez M., Fernandez-Diaz M.;
RT   "Cell Culture Contaminant.";
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-
CC         glutamyl-[protein] + methanol; Xref=Rhea:RHEA:23236, Rhea:RHEA-
CC         COMP:10208, Rhea:RHEA-COMP:10311, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:29973,
CC         ChEBI:CHEBI:82795; EC=3.1.1.61;
CC         Evidence={ECO:0000256|ARBA:ARBA00000941};
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DR   EMBL; CP029985; AXJ94240.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A345WKN8; -.
DR   KEGG; sphf:DM480_00745; -.
DR   OrthoDB; 9793421at2; -.
DR   Proteomes; UP000254856; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR   GO; GO:0008984; F:protein-glutamate methylesterase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-UniRule.
DR   CDD; cd16432; CheB_Rec; 1.
DR   CDD; cd17541; REC_CheB-like; 1.
DR   Gene3D; 3.40.50.2300; -; 1.
DR   Gene3D; 3.40.50.180; Methylesterase CheB, C-terminal domain; 1.
DR   InterPro; IPR008248; CheB-like.
DR   InterPro; IPR035909; CheB_C.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR000673; Sig_transdc_resp-reg_Me-estase.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   PANTHER; PTHR42872; PROTEIN-GLUTAMATE METHYLESTERASE/PROTEIN-GLUTAMINE GLUTAMINASE; 1.
DR   PANTHER; PTHR42872:SF6; PROTEIN-GLUTAMATE METHYLESTERASE_PROTEIN-GLUTAMINE GLUTAMINASE; 1.
DR   Pfam; PF01339; CheB_methylest; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PIRSF; PIRSF000876; RR_chemtxs_CheB; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF52172; CheY-like; 1.
DR   SUPFAM; SSF52738; Methylesterase CheB, C-terminal domain; 1.
DR   PROSITE; PS50122; CHEB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   4: Predicted;
KW   Chemotaxis {ECO:0000256|ARBA:ARBA00022500, ECO:0000256|PROSITE-
KW   ProRule:PRU00050}; Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00050}; Phosphoprotein {ECO:0000256|PROSITE-ProRule:PRU00169};
KW   Reference proteome {ECO:0000313|Proteomes:UP000254856}.
FT   DOMAIN          18..136
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000259|PROSITE:PS50110"
FT   DOMAIN          168..361
FT                   /note="CheB-type methylesterase"
FT                   /evidence="ECO:0000259|PROSITE:PS50122"
FT   ACT_SITE        180
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT   ACT_SITE        207
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT   ACT_SITE        304
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT   MOD_RES         69
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   361 AA;  36317 MW;  7CCE51D12938C2CF CRC64;
     MAVATPRLEP QDVDPPLRVL IVDDSAVARA VMTRIVEADA RFAVKGSVAT ASAALALLAQ
     ERVDLVLLDL QMPGIDGLTA LPDLIAAGRG AKILVVSAQA GEGAESTLQA LALGAADTLV
     KPAAGERMAA FGDGLIGKLV RLGHSGLDVP PLPAALPPIA PVSAAGRGRS DYDLVAIGAS
     TGGIHALSQL LRALPATFRV PILITQHLPA SFMPYFAAQI ATLSGRPCDV ATDLMRVQPG
     RIVVAPGDAH LRCVALPDGA AAIRLSHAPA ASGCLPSVDP MFASAAAVFG ARMLAIVLSG
     MGRDGAEGAK AVREAGGCVV VQDQTTSVVW GMPGAVAAIG AADAILTPDA IGRLAISGAR
     P
//