ID   A0A345XSK4_9ACTN        Unreviewed;       438 AA.
AC   A0A345XSK4;
DT   07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT   07-NOV-2018, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   SubName: Full=Alpha-glucosidase/alpha-galactosidase {ECO:0000313|EMBL:AXK34620.1};
GN   ORFNames=DVA86_20185 {ECO:0000313|EMBL:AXK34620.1};
OS   Streptomyces armeniacus.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=83291 {ECO:0000313|EMBL:AXK34620.1, ECO:0000313|Proteomes:UP000254425};
RN   [1] {ECO:0000313|EMBL:AXK34620.1, ECO:0000313|Proteomes:UP000254425}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15676 {ECO:0000313|EMBL:AXK34620.1,
RC   ECO:0000313|Proteomes:UP000254425};
RA   Labana P., Gosse J.T., Boddy C.N.;
RT   "Draft genome of the type strain Streptomyces armeniacus ATCC 15676.";
RL   Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|ARBA:ARBA00001936};
CC   -!- COFACTOR:
CC       Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC         Evidence={ECO:0000256|RuleBase:RU361152};
CC       Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU361152};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC       {ECO:0000256|ARBA:ARBA00010141, ECO:0000256|RuleBase:RU361152}.
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DR   EMBL; CP031320; AXK34620.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A345XSK4; -.
DR   Proteomes; UP000254425; Chromosome.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd05297; GH4_alpha_glucosidase_galactosidase; 1.
DR   Gene3D; 3.90.1820.10; AglA-like glucosidase; 1.
DR   InterPro; IPR001088; Glyco_hydro_4.
DR   InterPro; IPR022616; Glyco_hydro_4_C.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR32092; 6-PHOSPHO-BETA-GLUCOSIDASE-RELATED; 1.
DR   PANTHER; PTHR32092:SF6; ALPHA-GALACTOSIDASE; 1.
DR   Pfam; PF02056; Glyco_hydro_4; 1.
DR   Pfam; PF11975; Glyco_hydro_4C; 1.
DR   PRINTS; PR00732; GLHYDRLASE4.
DR   SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   3: Inferred from homology;
KW   Cobalt {ECO:0000256|PIRSR:PIRSR601088-3};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361152};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361152};
KW   Iron {ECO:0000256|PIRSR:PIRSR601088-3};
KW   Manganese {ECO:0000256|PIRSR:PIRSR601088-3};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR601088-3};
KW   NAD {ECO:0000256|RuleBase:RU361152};
KW   Nickel {ECO:0000256|PIRSR:PIRSR601088-3};
KW   Reference proteome {ECO:0000313|Proteomes:UP000254425}.
FT   DOMAIN          197..404
FT                   /note="Glycosyl hydrolase family 4 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF11975"
FT   BINDING         149
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT   BINDING         171
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT   BINDING         201
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT   SITE            111
FT                   /note="Increases basicity of active site Tyr"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR601088-4"
SQ   SEQUENCE   438 AA;  47900 MW;  29D0C3A025466E24 CRC64;
     MTGTKIVFVG AGSVVFTQGL LADLFAFSEL RDARIALHDI DPERLATAEG AARHIAAERG
     ATPVITAHPD RREALEGADF VINMIQVGMA AATRTDFDVP AGFGLRQTIG DTLGIGGIFR
     ALRTFPVLRA LAADMQAVCP DALLLNYTNP MAMNVMYLRE VAPRLRVVGL CHSVHWTMHD
     LSKLVGVPYE DVSYLAAGVN HQAWVLRFEH REDGRNLYPL LDEAIERDPQ LLRRVRVDMY
     RRLGHYPTET SEHSSEYVPW YLHHDSEVSR LRLPVGAYLG ITEENTASYE KTRAALAAGE
     PLPVEGTMEY APQIVHSVVT GTPRTVYGNV LNRGLIPELP AGAVVEVPCL VDSQGVQPTR
     IDALPPQCAA LNRSYVNVNE LTVRAATDGD PRHIRHAAMT DPATAAALPV DRIWSLCDTL
     VAAHGDLLQP NLRTPLGP
//