ID A0A348AN89_9FIRM Unreviewed; 3747 AA.
AC A0A348AN89;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=Polyketide synthase PksL {ECO:0000313|EMBL:BBB92537.1};
GN Name=pksL_1 {ECO:0000313|EMBL:BBB92537.1};
GN ORFNames=MAMMFC1_03232 {ECO:0000313|EMBL:BBB92537.1};
OS Methylomusa anaerophila.
OC Bacteria; Bacillota; Negativicutes; Selenomonadales; Sporomusaceae;
OC Methylomusa.
OX NCBI_TaxID=1930071 {ECO:0000313|EMBL:BBB92537.1, ECO:0000313|Proteomes:UP000276437};
RN [1] {ECO:0000313|EMBL:BBB92537.1, ECO:0000313|Proteomes:UP000276437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MMFC1 {ECO:0000313|EMBL:BBB92537.1,
RC ECO:0000313|Proteomes:UP000276437};
RX PubMed=29458677; DOI=10.1099/ijsem.0.002635;
RA Amano N., Yamamuro A., Miyahara M., Kouzuma A., Abe T., Watanabe K.;
RT "Methylomusa anaerophila gen. nov., sp. nov., an anaerobic methanol-
RT utilizing bacterium isolated from a microbial fuel cell.";
RL Int. J. Syst. Evol. Microbiol. 68:1118-1122(2018).
CC -!- FUNCTION: Involved in some intermediate steps for the synthesis of the
CC antibiotic polyketide bacillaene which is involved in secondary
CC metabolism. {ECO:0000256|ARBA:ARBA00003299}.
CC -!- PATHWAY: Antibiotic biosynthesis; bacillaene biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004789}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP018449; BBB92537.1; -; Genomic_DNA.
DR KEGG; mana:MAMMFC1_03232; -.
DR OrthoDB; 2460252at2; -.
DR UniPathway; UPA01003; -.
DR Proteomes; UP000276437; Chromosome.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 2.
DR CDD; cd08953; KR_2_SDR_x; 1.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 1.10.1240.100; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF08242; Methyltransf_12; 2.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000276437};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 407..484
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 557..972
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1901..1977
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2031..2470
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3588..3662
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 3747 AA; 419197 MW; 53B2BBC2095C34BB CRC64;
MRRMDELLCR LLWEQLKSIG LFSEKKATMA DLKMKISQHS LYDKWLEESL AILLRNSYLE
YDGEFYTTAG NMSVNMDGWL DWNLIKEEYS QNLNIKAHMV LVEAMLKALP DILTGKAEAR
NIIFPNSSME RLTQLEKYNK AAGYFNEVVA NTVSGYIQER IRQDSAAKLR IIEIGAGTGA
ANAIVFSKLS TYKQHIREYC YTDASRACLA HVQQEYGSDY LVCKLLDIDR PIAGQGVNSD
EYDILIAVNS LHAVKDIRQA LRNIKTLLRK NGLIVLNEIS ENSLFIHLTF GLLKEWCLYE
DSMLRIPGCA GLYPEKWQQV LESEGFQFIS FPATDAHNLG RQIIVAESDG VIRKKHDFTL
MPDSVGYDEN SSPINSDYCS KIPQDRIAIQ KNQPETNKAE MDVTEQTVNN FTRMILRESI
AGALKMETGK IKDELSFSEY GVDSIIAVNL VNAINKKLNI TMQITALFDY NNVNQLVRHI
VEEYESILIS MLQEQENMPG LAETGTEPAE AVAVNDSKWQ LSRFDGASFD FYPEMQTKYT
EQTMSSSVFK TSSPEQQDDI AIIGMSGRFA KSSTVKELWN NLKNGTNLIQ KVSRWDLSQY
FDQNRKYCNY GSFLEDIDKF DPLFFNISGL EATYMDPRQR IFLEEAWKAL EDAGYAGTAI
EGLKCGVYVG CGRTDYDQLF NDNPPPQAFW GNDNAVIPAR IAYYLNLKGP AVVVDTACSS
SLVAIHLASQ SLRAGETDLM LAGGIYLTCS PHTYLVGNRA EMLSYSGRCY TFDERADGFV
PGEGVGVVVL KRLKEAIADG DNIYGVIRGS GINQDGRTNG ITAPSALSQE RLERYVYETF
HIEPERIQMV EAHGTGTKLG DPIEFNALTR AFRAYTNRKQ YCAIGSIKTN LGHAVYAAGV
AGVIKVLLAM RHKKIPPTLN FQSGNPNINF EDSPFYVNTS LRYWDIDSNS KRCALVSSFG
FSGTNAHIAI EEPPAPARHY QEEPGYLIAV SARTPEQLRL QIKQLVAYCE ENDENIHCGD
ISYTLLLGRK HLHNRLACIV HDLQELVVLL KKWLSESSAP QVYYSVLHEN DLSEKESLKH
NGNQCIEKVK KANCEREFLE HLAAIAKLYI QGYKIEFDKL FSDNRYCRTS LPTYPFAKES
YWVPDNNTGT LTTAGLANKV INHPLLHQNT SDFFVQRFSS VFTGQEFFLA DHIVKGQRIL
PGVAYLEIAR AAIERAAGLL KTDENVISLK NVVWTRPLSV NAQAVQMSIE LILNENGEIS
YEVYSKPEAN VAELQIHSQG SAVLNPQAEI QALDIKAIKA KCQQGILTAG QCYDILGKMG
LNYGAGHQGI EQIYLGTGEV LAKLSLPSNV SNTLEQFVLH PSLLDSALQA SIGLRVSSTK
LLLPFALSEL EIRDNCTANM WAFICYSDGS TARDKVQKLD IDLCDEQGKI CVRMKGFSTR
ELDNEIDSKE ADSGTILLHP CWQERAISQL NKHPDYDQHI VVCCEAGEVS QKSIEAGIKG
VLCFILDSEQ ESIERRFKNY AVQLFEEIQS IFKRKPKEKI LLQVVISAKN EKQLFRGFSG
LLKTAQLENP QLKTQLIEAD NTEELAEKLL KNRRHSLDNH IRYQDGKRWV AGWSEVEEAE
QARTKIPWKD QGVYLISGGT GGLGLIFAKE IAEHGKGVTL ILTGSSPLNE GKQRKLEELQ
ALGAKIKYQQ TDVTKNEEVN SLIKAIINDY GKLNGIIHCA GVIDDNFIIK KTSAEFLQVQ
APKVTGLVNL DHASKDLPLD IFMLFSSVAG VIGNPGQADY SLANAFMDAY AQYRNELVAA
KRRYGQTLSI NWPLWQEGGM HLDEYTEKIF RKNTGIIPLE TAIGIKALYQ GLASAHEQVM
VVTGNAAKIR ELFVEQPSVA VHEENIKYAV NKTVSAVGAD LLREKVIHFF KKLLSTVIKL
PVQRIEADAP MEKYGIDSIM VMQMTNQLEN TFGSLPKTLF FEYQSIQEIV GYFLDTYREK
LVELLAAKNE TETFSNNPPI SPPAERPVSQ GLRNRIAPAI AKSRGKTAQG PFDIAIIGVS
GRYPGAKNIK EYWKNLQEGK DCISEIPKDR WDYSRYFDED KNKSGTIYSK WGGFIEGVDQ
FDPLFFNISP REAEFIDPQE RLFLQCVYET LEDAGYTRET VGMNREGGVD GNVGVYVGVM
YEEYQLYGAQ EQMMGRPIAL TGSPASIANR VSYFCNFNGP SIAIDTMCSS SLTAIHLACQ
SLQSGGCQLA IAGGVNISVH PNKYLMLSQG KFLSSKGRCE SFGQGGDGYV PGEGVGAVLL
KPLDRAVADG DHIYGVIKGI AVNHGGKTNG YTVPNPNAQA TVIGRALKQA RINPRIISYI
EAHGTGTFLG DPIEIAALSK TFQEYTADKQ FCAIGSAKSN IGHCESAAGI AGVTKVLLQL
KYHQLVPSLH SNVRNPNIDF GKTPFIVQQE LGEWKRPKAA LDGVMREYPR TAGISSFGAG
GSNAHVVIEE YIPNSREVQI PSSPAIIVLS AKNEEGLKEQ AKELLCAIEE QCFANTDLAS
MAYTLQVGRE AMEERLAMTV GSVQELEEKL RDFVQGKDNI DDLYYGQVKR NKETLAVLAT
DEDMTKAIDA WFSKRKYAKL LELWVKGLNI DWNRLYPDTK PERISLPTYP FAKKRCWVPG
DGNYQIDQIS LANIPNSWDG LMYVPIWEEQ PSTDIVKQRE PHAVLIVYHE SSSKLAEIIY
DHYSKNKLIE SILRVRLGNQ TKQVSQQEWI CDINDFESLK ICLKASGALD CLYFISECRD
DHSVPDFNAI IQSQQYNELQ LLRLIKQLKR QNGTDKQMDC YIISQDNYSV GAAGVNPRGG
GITGLAYSIA QGDHRFLVRN IDISQGDLST KQQQEELIRL ISNEAPTNRG ELVKIKAGLR
YKQEFRQLDT SAYTSQEPKF QRGGVYVILG GSGTVGGVIT RCLLQKYNSR VVWIGRKPAT
SDTIRKKLES LQAVGEPPLY VQADVTNLAE MKNALTTIKQ QYPVINGAIF AGIVFNSENS
VTKTDEAEFL KILNTKTKGS VNFYATFKDE PLDFMCYFSS AQAFSFSGAA NFSAYATGIT
FSDAFVQFIR RQSVFPIGII NWGFWKSSIV EAGEKLINQN TGTLQDKEGF ECFERFVNLL
QQGKLNQVIC LKATKSVLEL MKCKDNEVVS LNESSINSLI GCLWGNEKLV EQKLALLYKN
TSNTSEEINS WLAKMLFVQL RRMGLFRLGG IARDITLLRN STGIVNKYDR WLDECLNVLQ
MRGYIQCIED MIYPQEDWNF SDEELVTQQW EVVKEAYLKD ADWKAQVRLA AICLQRLPEI
LRGEILATDV MFPNSSMEMV EGIYKDNILS DFYNSIMADA VEEYVRQWVE AKPKTIVRII
EVGAGTGGTS KTVLARLKPY VDHVEYCYTD ISKSFLLFAE KQYGPDNPYL RYKLWNVEKP
VAAQGIEIGS YDIVIAANVL HATKNIRQTL RNIKAVLKRN GILLINEVTQ KSIFTTLTFG
LLNGWWLYED ENARITGSPL IRLEMWKKIL EEEGYRQVQL PTGSEENTGQ DIIAAESDGI
VRKELETRIV KEDIKENPVK GIAGVSDQSV HNDAIMSSIP TANGNADNYI ENAIRGLLAE
LLKVSLDDID GHVAFSDYGV DSIIGVSLVN RLNELLGTNI NSAVLFDYTT ADRLKDYLVK
TYKNQIKIQL PSHSSEGIPL QNDIKISDTL CQSTSDCTPG ISELGLSEQT EISMIKDLQN
RFFSNEISTD LLVETLSNKY NRGVNNG
//
