ID   A0A363UQS9_9GAMM        Unreviewed;       378 AA.
AC   A0A363UQS9;
DT   07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT   07-NOV-2018, sequence version 1.
DT   24-JAN-2024, entry version 19.
DE   SubName: Full=Osmoprotectant NAGGN system M42 family peptidase {ECO:0000313|EMBL:PWN57867.1};
GN   ORFNames=DEH80_01655 {ECO:0000313|EMBL:PWN57867.1};
OS   Abyssibacter profundi.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Salinisphaerales;
OC   Salinisphaeraceae; Abyssibacter.
OX   NCBI_TaxID=2182787 {ECO:0000313|EMBL:PWN57867.1, ECO:0000313|Proteomes:UP000251800};
RN   [1] {ECO:0000313|EMBL:PWN57867.1, ECO:0000313|Proteomes:UP000251800}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OUC007 {ECO:0000313|EMBL:PWN57867.1,
RC   ECO:0000313|Proteomes:UP000251800};
RA   Zhou S.;
RT   "Abyssibacter profundi OUC007T gen. nov., sp. nov, a marine bacterium
RT   isolated from seawater of the Mariana Trench.";
RL   Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001123-2};
CC       Note=Binds 2 divalent metal cations per subunit.
CC       {ECO:0000256|PIRSR:PIRSR001123-2};
CC   -!- SIMILARITY: Belongs to the peptidase M42 family.
CC       {ECO:0000256|ARBA:ARBA00006272, ECO:0000256|PIRNR:PIRNR001123}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:PWN57867.1}.
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DR   EMBL; QEQK01000001; PWN57867.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A363UQS9; -.
DR   OrthoDB; 361940at2; -.
DR   Proteomes; UP000251800; Unassembled WGS sequence.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd05657; M42_glucanase_like; 1.
DR   Gene3D; 2.40.30.40; Peptidase M42, domain 2; 1.
DR   Gene3D; 3.40.630.10; Zn peptidases; 1.
DR   InterPro; IPR008007; Peptidase_M42.
DR   InterPro; IPR023367; Peptidase_M42_dom2.
DR   InterPro; IPR017537; Peptidase_M42_hydrolase.
DR   NCBIfam; TIGR03106; trio_M42_hydro; 1.
DR   PANTHER; PTHR32481; AMINOPEPTIDASE; 1.
DR   PANTHER; PTHR32481:SF7; AMINOPEPTIDASE YHFE-RELATED; 1.
DR   Pfam; PF05343; Peptidase_M42; 1.
DR   PIRSF; PIRSF001123; PepA_GA; 1.
DR   SUPFAM; SSF101821; Aminopeptidase/glucanase lid domain; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000256|ARBA:ARBA00022438};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR001123-2}; Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000251800}.
FT   ACT_SITE        224
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-1"
FT   BINDING         72
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         190
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         190
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
FT   BINDING         225
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001123-2"
SQ   SEQUENCE   378 AA;  41829 MW;  D6FDF369E5D638D0 CRC64;
     MKRLPIDNEY LRDHLLTLLN IPSPTGFTDE ITTYVGKELE RLGVPLELTR RGAIRATISG
     DVSSPDRAVV VHLDTIGAMV RAFKDNGRLE VTPIGTWSAR FAEGARCSIL LDDGRQLRGT
     ILPLKASGHT FNEEVDTQAT SWDNLELRVD EFAYSEADLR KLGIQIGNFL SIDPNPEISE
     SGFINSRHLD DKAGVAALLA ATKAIVESGI RPPVDCHLLF TISEEVGYGA SAVLHQDVAE
     MVTIDNATPA PGQNSKETGV TIAIKDQVGP FDYHLTRKLT EICEEHEVRY QRDVFRYYRS
     DSASAIEAGN DIRTALLCFG VDASHGYERT HLHSLRSLAE LLALYIQSRP TFLRDREELA
     PLEGFPEQTL EQDEGEAN
//