ID   A0A364K2G5_9BACL        Unreviewed;       859 AA.
AC   A0A364K2G5;
DT   07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT   07-NOV-2018, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE            EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN   ORFNames=DL897_13145 {ECO:0000313|EMBL:RAL22612.1};
OS   Thermoflavimicrobium daqui.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Thermoactinomycetaceae;
OC   Thermoflavimicrobium.
OX   NCBI_TaxID=2137476 {ECO:0000313|EMBL:RAL22612.1, ECO:0000313|Proteomes:UP000251213};
RN   [1] {ECO:0000313|EMBL:RAL22612.1, ECO:0000313|Proteomes:UP000251213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FBKL4.011 {ECO:0000313|EMBL:RAL22612.1,
RC   ECO:0000313|Proteomes:UP000251213};
RA   Wang X., Zhou H.;
RT   "Thermoflavimicrobium daqus sp. nov., a thermophilic microbe isolated from
RT   Moutai-flavour Daqu.";
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:RAL22612.1, ECO:0000313|Proteomes:UP000251213}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FBKL4.011 {ECO:0000313|EMBL:RAL22612.1,
RC   ECO:0000313|Proteomes:UP000251213};
RA   Zhirakovskaya E.;
RL   Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00034618};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RAL22612.1}.
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DR   EMBL; QJKK01000008; RAL22612.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A364K2G5; -.
DR   OrthoDB; 7066673at2; -.
DR   Proteomes; UP000251213; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR   CDD; cd18807; SF1_C_UvrD; 1.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR007560; Restrct_endonuc_IV_Mrr.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR   PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR   Pfam; PF04471; Mrr_cat; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000251213}.
FT   DOMAIN          232..510
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51198"
FT   BINDING         253..260
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ   SEQUENCE   859 AA;  99815 MW;  E35ADF736998EF57 CRC64;
     MSLVAIQDTF LEAYAELPKS VQQLVQKNIR LLREQKENEL KMERYKDAID SKVFLIRTGP
     HHSMVVVRPE EEKATLLAWV DRDESAYLWA KRKRFVINQY TGSLQMWTDV EVKGEISEQP
     NLFADYTDDQ LLRLGIPDNQ LPLLRSITTE QEFRKYKRDL PLEAFENLAR LLNGTPFEIV
     VESVQEYKKP EEHLSYEAAM KSAYASRRIV VITNDKDLNP ILDKPLEWWR IFLHPDQREL
     VEKDYKGPVR VLGGAGTGKT VVVMHRAHHL VQNKLKSTER ILVTTYTKIL ANNIREVLAT
     MCSEEEMEHI DIRPIDDLAK EIVEKAYKNQ ARIVDKDDVK VNAIWKEAIK EFGWEPEDLY
     FLKNEYHWVI QQGGAESWQE YLNTARAGRK KRITRELRKK IWDTAMWFRQ KMEEKDLFEY
     IDILRKARNW LHENPDRYTY TYRFALIDEA QDFHPEAFKM IRALVPEGDN DLFIVGDAHQ
     RIYSRKLVLS KCGIHVRGRS KRLTVNYRTT EEIREKAIDI INYTVDDLEK GIDTGIDTSL
     LSGTKPEIRN FVDAQAEREF VIQKVKELLS DEIKPNEIAI LARFDHTVDR YLKEFEEQRI
     QAQKLSASMK SDQPKIYCGT MHNSKGLEFK IVFLVEANEE MIPPEWELES LEDEDDKTEL
     MKLERSLLYV AMTRARDKVY ITSFGALTKF LQENPKDDKE TDQPKKVQTK ESIILSESEI
     EELIHQVLSC SPDFFEKLVI QLLSKMGYGG SVPDSAFVVG GTGDEGIDGI ILEDPLGLHK
     IYVQAKRWKN SVGRPEIQKF AGALIGKNAE KGVFITTSRF TIPAKKYAEQ IPQNMVLIDG
     TELAKLLNKY NMKIAEIHQ
//