ID A0A365N5Y3_GIBIN Unreviewed; 400 AA.
AC A0A365N5Y3;
DT 07-NOV-2018, integrated into UniProtKB/TrEMBL.
DT 07-NOV-2018, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=Protein phosphatase methylesterase 1 {ECO:0000256|ARBA:ARBA00020672, ECO:0000256|PIRNR:PIRNR022950};
DE Short=PME-1 {ECO:0000256|PIRNR:PIRNR022950};
DE EC=3.1.1.- {ECO:0000256|PIRNR:PIRNR022950};
GN ORFNames=BFJ72_g7636 {ECO:0000313|EMBL:RKL37551.1}, FPRO05_11565
GN {ECO:0000313|EMBL:RBA16230.1};
OS Gibberella intermedia (Bulb rot disease fungus) (Fusarium proliferatum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=948311 {ECO:0000313|EMBL:RBA16230.1, ECO:0000313|Proteomes:UP000251714};
RN [1] {ECO:0000313|EMBL:RBA16230.1, ECO:0000313|Proteomes:UP000251714}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ITEM 2341 {ECO:0000313|EMBL:RBA16230.1,
RC ECO:0000313|Proteomes:UP000251714};
RA Almiman B.F., Shittu T.A., Muthumeenakshi S., Baroncelli R.,
RA Sreenivasaprasada S.;
RT "Genome sequence of the mycotoxigenic crop pathogen Fusarium proliferatum,
RT strain ITEM 2341 from Date Palm.";
RL Submitted (DEC-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:RKL37551.1, ECO:0000313|Proteomes:UP000283569}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Fp_A8 {ECO:0000313|EMBL:RKL37551.1,
RC ECO:0000313|Proteomes:UP000283569};
RX PubMed=30202022; DOI=10.1038/s41598-018-30335-7;
RA Armitage A.D., Taylor A., Sobczyk M.K., Baxter L., Greenfield B.P.,
RA Bates H.J., Wilson F., Jackson A.C., Ott S., Harrison R.J., Clarkson J.P.;
RT "Characterisation of pathogen-specific regions and novel effector
RT candidates in Fusarium oxysporum f. sp. cepae.";
RL Sci. Rep. 8:13530-13530(2018).
CC -!- FUNCTION: Demethylates proteins that have been reversibly
CC carboxymethylated. Demethylates the phosphatase PP2A catalytic subunit.
CC {ECO:0000256|ARBA:ARBA00024741}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[phosphatase 2A protein]-C-terminal L-leucine methyl ester +
CC H2O = [phosphatase 2A protein]-C-terminal L-leucine + H(+) +
CC methanol; Xref=Rhea:RHEA:48548, Rhea:RHEA-COMP:12134, Rhea:RHEA-
CC COMP:12135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790,
CC ChEBI:CHEBI:90516, ChEBI:CHEBI:90517; EC=3.1.1.89;
CC Evidence={ECO:0000256|ARBA:ARBA00000906};
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily.
CC {ECO:0000256|ARBA:ARBA00008645, ECO:0000256|PIRNR:PIRNR022950}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RBA16230.1}.
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DR EMBL; PKMI01000018; RBA16230.1; -; Genomic_DNA.
DR EMBL; MRDB01000025; RKL37551.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A365N5Y3; -.
DR VEuPathDB; FungiDB:FPRN_00984; -.
DR Proteomes; UP000251714; Unassembled WGS sequence.
DR Proteomes; UP000283569; Unassembled WGS sequence.
DR GO; GO:0051723; F:protein methylesterase activity; IEA:InterPro.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR016812; PPase_methylesterase_euk.
DR PANTHER; PTHR14189:SF0; PROTEIN PHOSPHATASE METHYLESTERASE 1; 1.
DR PANTHER; PTHR14189; PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED; 1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR PIRSF; PIRSF022950; PPase_methylesterase_euk; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|PIRNR:PIRNR022950};
KW Serine esterase {ECO:0000256|PIRNR:PIRNR022950}.
FT DOMAIN 119..369
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000259|Pfam:PF12697"
FT REGION 18..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..37
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 202
FT /evidence="ECO:0000256|PIRSR:PIRSR022950-1"
FT ACT_SITE 228
FT /evidence="ECO:0000256|PIRSR:PIRSR022950-1"
FT ACT_SITE 358
FT /evidence="ECO:0000256|PIRSR:PIRSR022950-1"
SQ SEQUENCE 400 AA; 43717 MW; 32AF00BDB8E4EFAE CRC64;
MSDLQRAWAK AKYSISNDTF DEVDEEHEND VLPELPEPVD DDSSSASSAS SVSSTGTIIP
SPNQKLFARP QGVARGRTLE QIPWTTYFER ELSLKSEQEP EVVYHAYLTS PVGKGPLFVM
HHGAGSSGLS FAVVASEIKK RLSTAGILAI DCRGHGSTSA PGDKALDMRL DTLSSDLFSM
VQLTKNEMAW PEMPPIVLVG HSLGGAVVTD LAKSGRLGTS VLGYAVLDVV EGSAIDALQS
MHTYLSTRPL GFATLQAGIE WHIRSRTIRN SISARTSVPA LLVFNENDDP TRPWRWRTNL
GATQPYWEGW FIGLSKKFLG AKGGKLLLLA GTDRLDTELT IGQMQGKYAL QVFPEAGHFI
HEDLPEKTAV SLVDFFRRND RSALVLPPKV SDLLKQGKRV
//
